First Report of <i>Neopestalotiopsis clavispora</i> Causing Leaf Spot on Macadamia (<i>Macadamia integrifolia</i>) in China

Qiu, F.; Xu, Gang; Zheng, F. Q.; Zhou, Jia; Zheng, Lianwen; Miao, Weiguo; Xie, Changping

doi:10.1094/pdis-07-19-1367-pdn

Cited by 8 publications

(5 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…rosae causes dieback, crown rot, fruit rot and root rot of many economically important plants including Eucalyptus , Fragaria , Paeonia and Vitis ( Maharachchikumbura et al 2014 , Rebollar-Alviter et al 2020 , Santos et al 2020 , Cosseboom & Hu 2021 ), and N . clavispora can infect more than 50 plant species belonging to 27 families in China and about 17 other countries, resulting in severe leaf diseases ( Qiu et al 2020 ). For this reason, the two new species isolated from Rosa may present dangerous pathogens for other hosts, and further work is needed to better understand the role and distribution of these two new Neopestalotiopsis species.…”

Section: Discussionmentioning

confidence: 99%

Diversity ofSporocadaceae(pestalotioid fungi) fromRosain China

Peng¹,

Crous²,

Jiang³

et al. 2022

persoonia

View full text Add to dashboard Cite

Rosa (Rosaceae) is an important ornamental and medicinal plant genus worldwide, with several species being cultivated in China. Members of Sporocadaceae (pestalotioid fungi) are globally distributed and include endophytes, saprobes but also plant pathogens, infecting a broad range of host plants on which they can cause important plant diseases. Although several Sporocadaceae species were recorded to inhabit Rosa spp., the taxa occurring on Rosa remain largely unresolved. In this study, a total of 295 diseased samples were collected from branches, fruits, leaves and spines of eight Rosa species (R. chinensis, R. helenae, R. laevigata, R. multiflora, R. omeiensis, R. rugosa, R. spinosissima and R. xanthina) in Gansu, Henan, Hunan, Qinghai, Shaanxi Provinces and the Ningxia Autonomous Region of China. Subsequently 126 strains were obtained and identified based on comparisons of DNA sequence data. Based on these results 15 species residing in six genera of Sporocadaceae were delineated, including four known species (Pestalotiopsis chamaeropis, Pes. rhodomyrtus, Sporocadus sorbi and Spo. trimorphus) and 11 new species described here as Monochaetia rosarum, Neopestalotiopsis concentrica, N. subepidermalis, Pestalotiopsis tumida, Seimatosporium centrale, Seim. gracile, Seim. nonappendiculatum, Seim. parvum, Seiridium rosae, Sporocadus brevis, and Spo. spiniger. This study also represents the first report of Pes. chamaeropis, Pes. rhodomyrtus and Spo. sorbi on Rosa. The overall data revealed that Pestalotiopsis was the most prevalent genus, followed by Seimatosporium, while Pes. chamaeropis and Pes. rhodomyrtus were the two most prevalent species. Analysis of Sporocadaceae abundance on Rosa species and plant organs revealed that spines of R. chinensis had the highest species diversity.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity ofSporocadaceae(pestalotioid fungi) fromRosain China

Peng¹,

Crous²,

Jiang³

et al. 2022

persoonia

View full text Add to dashboard Cite

show abstract

“…Symptoms are expressed as blight and necrosis of the flowers and dieback of the rachis (Akinsanmi et al, 2017). Other species of Pestalotiopsis and Neopestalotiopsis, such as P. versicolor and N. clavispora, cause leaf spots in macadamia (Prasannath et al, 2020;Qiu et al, 2019;Santos et al, 2019). Prasannath et al (2020) suggested that the leaf spot pathogens can also cause dry flower disease, making management challenging.…”

Section: Cladosporium Cladosporioides (Van Denmentioning

confidence: 99%

“…versicolor and N . clavispora , cause leaf spots in macadamia (Prasannath et al, 2020; Qiu et al, 2019; Santos et al, 2019). Prasannath et al (2020) suggested that the leaf spot pathogens can also cause dry flower disease, making management challenging.…”

Section: Introductionmentioning

confidence: 99%

Molecular methods for the detection and quantification of Pestalotiopsis and Neopestalotiopsis inoculum associated with macadamia

2021

View full text Add to dashboard Cite

Pestalotiopsis blight and leaf spot in macadamia are caused by species of Pestalotiopsis and Neopestalotiopsis. Conidia produced by these pathogens are mostly dispersed by wind and rain splash and are the primary infecting propagules. Rapid detection and quantification of airborne conidia are essential to determine the seasonal dynamics of inoculum and critical infection periods in macadamia orchards. A quantitative PCR (qPCR) using a new primer pair and a hydrolysis probe was developed to detect and quantify airborne conidia of Pestalotiopsis and Neopestalotiopsis species. The amplification efficiency of the qPCR was 96.4% and the assay had a limit of detection of 400 fg of Pestalotiopsis/Neopestalotiopsis gDNA, which corresponds to approximately 10 conidia. The qPCR assay coupled with spore trapping was used to monitor airborne conidia dispersal under field conditions. The results showed that the assay is specific and sensitive for detecting and quantifying Pestalotiopsis/Neopestalotiopsis conidia in macadamia orchards. The detection and quantification of the pathogen inoculum will improve our understanding of disease epidemiology and the ability to manage these diseases in macadamia.

show abstract

“…Several species of Neopestalotiopsis are phytopathogens in tropical and subtropical regions, causing leaf spot, dry flower, fruit rot, fruit scab, and trunk diseases on a range of crops [ 5 , 8 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Flower and leaf diseases on macadamia caused by Neopestalotiopsis spp.…”

Section: Introductionmentioning

confidence: 99%

“…Flower and leaf diseases on macadamia caused by Neopestalotiopsis spp. have been reported in Australia [5,8], Brazil [26], and China [25]. Many new pestalotioid species have been introduced in recent years [27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Neopestalotiopsis Species Associated with Flower Diseases of Macadamia integrifolia in Australia

Prasannath

Shivas

Galea

et al. 2021

JoF

View full text Add to dashboard Cite

Macadamia (Macadamia integrifolia) is native to eastern Australia and produces an edible nut that is extensively cultivated in commercial orchards in several countries. Little is known about the diversity of fungi associated with diseases of macadamia inflorescences. A survey of fungi associated with the dry flower disease of macadamia detected several isolates of Neopestalotiopsis (Pestalotiopsidaceae, Sordariomycetes). Five new species of Neopestalotiopsis were identified based on molecular phylogenetic analyses of concatenated gene sequences of the internal transcribed spacer (ITS), β-tubulin (TUB), and the translation elongation factor 1-alpha (TEF1α). The new species are named Neopestalotiopsis drenthii, N. maddoxii, N. olumideae, N. vheenae, and N. zakeelii, and are described by molecular, morphological, and cultural characteristics. The ecology of the isolates and their pathogenic, saprophytic, or commensal ability were not determined.

show abstract

First Report of Neopestalotiopsis clavispora Causing Leaf Spot on Macadamia (Macadamia integrifolia) in China

Cited by 8 publications

References 1 publication

Diversity ofSporocadaceae(pestalotioid fungi) fromRosain China

Diversity ofSporocadaceae(pestalotioid fungi) fromRosain China

Molecular methods for the detection and quantification of Pestalotiopsis and Neopestalotiopsis inoculum associated with macadamia

Neopestalotiopsis Species Associated with Flower Diseases of Macadamia integrifolia in Australia

Contact Info

Product

Resources

About