First Report of Maize yellow mosaic virus Infecting Sugarcane (Saccharum spp.) and Itch Grass (Rottboellia cochinchinensis) in Nigeria

Yahaya, Abubakar; Rwahnih, Maher Al; Dangora, D. B.; Gregg, Lori L.; Alegbejo, M. D.; Kumar, P. Lava; Alabi, Olufemi J.

doi:10.1094/pdis-03-17-0315-pdn

Cited by 28 publications

(10 citation statements)

References 3 publications

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“…The MinION sequence data for MYMV was compared with that generated using the MiSeq and the sequences from Genbank. The results (Figure 1 MYMV was first described and named in China in 2016 29 , followed by the sequencing of the whole genome 28 and has recently been reported in Nigeria 30 . The symptoms are described as "yellow mosaic" 28 or "yellowing and dwarfing" 29 .…”

Section: Resultsmentioning

confidence: 91%

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

Adams

Braidwood

Stomeo³

et al. 2017

Preprint

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Maize lethal necrosis disease (MLN) is an emerging disease in East Africa caused by the introduction of Maize chlorotic mottle virus (MCMV). Recent activity seeking to limit spread of the disease is reliant on effective diagnostics. Traditional diagnostics applied on samples with typical field symptoms of MLN have often given negative results using ELISA or PCR for MCMV and Sugarcane mosaic virus (SCMV). Samples collected in the field with typical MLN symptoms were examined using next generation sequencing (NGS). SCMV was found to be more prevalent than suggested by targeted diagnostics. Additionally, the panel of samples were found to be infected with a range of other viruses, seven of which are described here for the first time. Although not previously identified in the region, Maize yellow mosaic virus (MYMV) was the most prevalent virus after MCMV. The development of targeted diagnostics for emerging viruses is complicated when the extent of field variation is unknown, something that can be negated by using NGS methods. As a result we explored MinION technology which may be more readily deployable in resource poor settings. The results show that this sequencer can diagnose known viruses and future iterations have the potential to identify novel viruses.

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Section: Resultsmentioning

confidence: 91%

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

Adams

Braidwood

Stomeo³

et al. 2017

Preprint

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“…In 2013, the first species was named Maize yellow dwarf virus (MYDV-RMV) [ 50 ]. Two different isolates from China were named Maize yellow mosaic virus (MaYMV) [ 46 ] and Maize yellow dwarf virus -RMV2 (MYDV-RMV2) [ 51 ], while an isolate infecting sugarcane in Nigeria was named Maize yellow mosaic virus (MaYMV) [ 52 ], and an isolate infecting maize in Kenya was renamed as Maize yellow dwarf virus -RMV (MF974579.2). Three near genome length polerovirus contigs from maize and one from sorghum described here were most closely related (99% similarity) to Maize yellow dwarf virus -RMV (Figs.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya

Wamaitha

Nigam

Maina

et al. 2018

Virol J

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BackgroundMaize lethal necrosis is caused by a synergistic co-infection of Maize chlorotic mottle virus (MCMV) and a specific member of the Potyviridae, such as Sugarcane mosaic virus (SCMV), Wheat streak mosaic virus (WSMV) or Johnson grass mosaic virus (JGMV). Typical maize lethal necrosis symptoms include severe yellowing and leaf drying from the edges. In Kenya, we detected plants showing typical and atypical symptoms. Both groups of plants often tested negative for SCMV by ELISA.MethodsWe used next-generation sequencing to identify viruses associated to maize lethal necrosis in Kenya through a metagenomics analysis. Symptomatic and asymptomatic leaf samples were collected from maize and sorghum representing sixteen counties.ResultsComplete and partial genomes were assembled for MCMV, SCMV, Maize streak virus (MSV) and Maize yellow dwarf virus-RMV (MYDV-RMV). These four viruses (MCMV, SCMV, MSV and MYDV-RMV) were found together in 30 of 68 samples. A geographic analysis showed that these viruses are widely distributed in Kenya. Phylogenetic analyses of nucleotide sequences showed that MCMV, MYDV-RMV and MSV are similar to isolates from East Africa and other parts of the world. Single nucleotide polymorphism, nucleotide and polyprotein sequence alignments identified three genetically distinct groups of SCMV in Kenya. Variation mapped to sequences at the border of NIb and the coat protein. Partial genome sequences were obtained for other four potyviruses and one polerovirus.ConclusionOur results uncover the complexity of the maize lethal necrosis epidemic in Kenya. MCMV, SCMV, MSV and MYDV-RMV are widely distributed and infect both maize and sorghum. SCMV population in Kenya is diverse and consists of numerous strains that are genetically different to isolates from other parts of the world. Several potyviruses, and possibly poleroviruses, are also involved.Electronic supplementary materialThe online version of this article (10.1186/s12985-018-0999-2) contains supplementary material, which is available to authorized users.

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“…NGS was also used to identify Johnsongrass mosaic virus (JGMV) in samples from Kenya and Uganda, and further experiments demonstrated a role for this virus in causing MLN (Stewart et al 2017). A new polerovirus, tentatively named maize yellow mosaic virus, was identified in southwestern China using NGS (Chen et al 2016) and was subsequently found in maize from southeastern China, Ecuador, and sub-Saharan Africa (Bernreiter et al 2017;Palanga et al 2017;Wang et al 2016, Stewart et al unpublished results). Similarly, a genome sequence related to fungal totiviruses was identified in maize (Chen et al 2016).…”

Section: Genome Sequencing For Virus Diagnostics and Characterizationmentioning

confidence: 99%

The Genetics and Genomics of Virus Resistance in Maize

Redinbaugh

Lübberstedt

Leng

et al. 2018

Compendium of Plant Genomes

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Viruses cause significant diseases on maize worldwide. Intensive agronomic practices, changes in vector distribution, and the introduction of vectors and viruses into new areas can result in emerging disease problems. Because deployment of resistant hybrids and cultivars is considered to be both economically viable and environmentally sustainable, genes and quantitative trait loci for most economically important virus diseases have been identified. Examination of multiple studies indicates the importance of regions of maize chromosomes 2, 3, 6, and 10 in virus resistance. An understanding of the molecular basis of virus resistance in maize is beginning to emerge, and two genes conferring resistance to sugarcane mosaic virus, Scmv1 and Scmv2, have been cloned and characterized. Recent studies provide hints of other pathways and genes critical to virus resistance in maize, but further work is required to determine the roles of these in virus susceptibility and resistance. This research will be facilitated by rapidly advancing technologies for functional analysis of genes in maize.

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First Report of Maize yellow mosaic virus Infecting Sugarcane (Saccharum spp.) and Itch Grass (Rottboellia cochinchinensis) in Nigeria

Cited by 28 publications

References 3 publications

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

Characterising maize viruses associated with maize lethal necrosis symptoms in sub Saharan Africa

Metagenomic analysis of viruses associated with maize lethal necrosis in Kenya

The Genetics and Genomics of Virus Resistance in Maize

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