First report of <i>Fusarium proliferatum</i> causing rot of garlic bulbs in North America

Dugan, Frank M.; Hellier, Barbara C.; Lupien, S. L.

doi:10.1046/j.1365-3059.2003.00852.x

Cited by 56 publications

(51 citation statements)

References 3 publications

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“…Valdez, personal communication). In garlic, this species was reported in Hungary (Simay 1990) and USA (Dugan et al 2003). In addition to these reports, our results support that F. proliferatum is another relevant species causing Fusarium basal rot.…”

Section: Discussionsupporting

confidence: 88%

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Genetic variation among Fusarium isolates from onion, and resistance to Fusarium basal rot in related Allium species

et al. 2008

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The aim of this research was to study levels of resistance to Fusarium basal rot in onion cultivars and related Allium species, by using genetically different Fusarium isolates. In order to select genetically different isolates for disease testing, a collection of 61 Fusarium isolates, 43 of them from onion (Allium cepa), was analysed using amplified fragment length polymorphism (AFLP) markers. Onion isolates were collected in The Netherlands (15 isolates) and Uruguay (9 isolates), and received from other countries and fungal collections (19 isolates).

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Section: Discussionsupporting

confidence: 88%

“…Recently, F. proliferatum was found affecting onion (du Toit et al 2003;Stankovic et al 2007) and garlic (Dugan et al 2003). Other Fusarium species were reported in the past as minor onion pathogens (Entwistle 1990), but F. oxysporum is the most frequently found species causing onion basal rot.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation among Fusarium isolates from onion, and resistance to Fusarium basal rot in related Allium species

et al. 2008

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“…Fusarium proliferatum, a species that is easily misidentified as F. verticillioides because of its closely related morphology (Nelson et al 1983;Leslie et al 1996), has been isolated from Allium spp., including bulb onion and garlic in different countries (du Toit et al 2003;Dugan et al 2003;Stankovic et al 2007). Therefore, in the present study we used species-specific primers to confirm the identity of F. verticillioides.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan

et al. 2008

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Thirty-two isolates of Fusarium species were obtained from wilted Welsh onion (Allium fistulosum) grown on nine farms from six regions in Japan and identified as F. oxysporum (18 isolates), F. verticillioides (7 isolates), and F. solani (7 isolates). The pathogenicity of 32 isolates was tested on five commercial cultivars of Welsh onion and two cultivars of bulb onion in a seedling assay in a greenhouse. The Fusarium isolates varied in the degree of disease severity on the cultivars. Five F. oxysporum isolates (08, 15, 17, 22, and 30) had a higher virulence on the cultivars than the other isolates. The host range of these five isolates was limited to Allium species. Molecular characterization of Fusarium isolates was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer (ITS) regions of ribosomal DNA. The 32 isolates were grouped into eight types (four types for F. oxysporum, one for F. verticillioides, and three for F. solani). Restriction patterns of the ITS region were not related to pathogenicity. However, the haplotypes obtained with five enzymes (RsaI, HinfI, HaeIII, ScrFI, and MspI) and the phylogenetic analysis permitted the discernment of the three Fusarium species. The PCR-RFLP analysis should provide a rapid, simple method for differentiating Fusaruim species isolated from wilted Welsh onion in Japan.

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“…Fusarium proliferatum is known to cause diseases like Fusarium crown and root rot of asparagus [5], leaf spot of cymbidium [6], and rot of garlic bulb [7].To our knowledge this is the first report of Fusarium proliferatum causing wilting of sorrel in Maharashtra State, India.…”

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confidence: 83%

First Report of Fusarium proliferatum Inciting Wilt of Rumex acetosa L. in Maharashtra, India

Bhale¹,

Chatage²,

Ambuse³

2012

J Plant Pathol Microbiol

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Sequencing.Rumex acetosa L. (sorrel) belongs to family Polygonaceae. It is an indigenous English plant, common, too in the greater part of Europe, in almost all soils and situations. The medicinal action of sorrel is refrigerant and diuretic, febrile disorders and in scurvy. In India it is cultivated as a medicinal and vegetable throughout the year. Both the root and the seed were formerly esteemed for their astringent properties, and were employed to stem haemorrhage [1]. During October 2009 to January 2011 an extensive surveys was conducted in sorrel growing areas of Marathwada region of Maharashtra, India where diseased plants were found with yellow discoloration of the vascular tissue in stems, wilting of the lower leaves & root rot (Figure1). Roots from wilted plants were collected and cut into small pieces (2mm) and then surface sterilized with mercuric chloride (1% HgCl 2 ) for 1 min. The pieces were then washed with sterilized distilled water three times, blotted dry and plated on potato dextrose agar (PDA) medium and incubated at 27±2 o C in BOD incubator. On PDA medium, Fusarium species with catenate microconidia and macroconidia borne on polyphialides was consistently isolated from the infected roots. Pathogenicity tests were conducted, using six healthy sorrel plants grown in the glasshouse. Two plants and a control were used for each of two replications. For each treated plants were sprayed with 5 ml conidia suspension (ca.1×10 5 conidia/ml) in sterilized water (SDW) and maintained in a humid growth chamber for 24 h in room temperature. Five days after inoculation, white mycelial roots were infected and in control remained symptomless. The fungus was reisolated & was identical as Fusarium species. The fungal culture is deposited at CODON Life Sciences, Goa and Research laboratory, Department of Botany Arts, Science & Commerce College Naldurg Dist Osmanbad, Maharashtra State, India with Accession No.HQ332533.Thirty isolates were identified as Fusarium proliferatum (Matsushima) Nirenberg [2,3].Cultural colonies were started from single conidium and grown on PDA at 27±2 o C under fluorescent lights providing 12 hrs light period. The abundant aerial mycelium initially was white and later became purple violet. Colonies were fast growing, hyphae were septate and hyaline. Conidiophores were short, simple and branched. Microconidia were abundant and produced on mono and polyphialids, single celled, oval to club shaped size 7.0-22.5×3.5µm. Macroconidia were slightly sickle shaped to straight, with 2-5 septa and measured 43-65×3.3-5.0µm. Chlamydospores were absent (Figure 1).

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First report of Fusarium proliferatum causing rot of garlic bulbs in North America

Cited by 56 publications

References 3 publications

Genetic variation among Fusarium isolates from onion, and resistance to Fusarium basal rot in related Allium species

Genetic variation among Fusarium isolates from onion, and resistance to Fusarium basal rot in related Allium species

Pathogenic variation and molecular characterization of Fusarium species isolated from wilted Welsh onion in Japan

First Report of Fusarium proliferatum Inciting Wilt of Rumex acetosa L. in Maharashtra, India

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