First report of <i>Dasheen mosaic virus</i> in elephant foot yam in India

Pandit, M. K.; Nath, Partha Sarathi; Mukhopadhyay, Sibabrata; Devonshire, B. Jean; Jones, Philip W.

doi:10.1046/j.1365-3059.2001.00625.x

Cited by 16 publications

(6 citation statements)

References 2 publications

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“…In the case of Elephant foot yam, occurrences of mosaic, puckering, leaf distortion, filiformy or shoestringing type symptoms are common. In India, Pandit et al (2001), first reported the association of a DsMV with the mosaic disease of Elephant foot yam based on serological studies from the leaf samples collected from West Bengal. The infection caused by DsMV on the plant appeared to be severe resulting in reduced tuber yields.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Babu¹,

Hegde²

2014

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Summary. -Dasheen mosaic virus (DsMV) infecting three major edible aroids namely Amorphophallus paeoniifolius, Colocasia esculenta, and Xanthosoma sagittifolium cultivated in India was characterized. Infected plants showing typical DsMV symptoms were subjected to reverse transcription-polymerase chain reaction, and an amplification of a 963 bp fragment which encoded the coat protein (CP) gene was obtained. BLAST analysis of the cloned DNA amplicon revealed the identity of the virus to be that of DsMV. Sequence identity matrix of the nucleotide sequences among the three isolates showed that the DsMV isolate infecting A. paeoniifolius and C. esculenta shared an identity as high as 93%, while the DsMV isolate from X. sagittifolium shared an identity of only 73% and 76% with the DsMV isolates from A. paeoniifolius and C. esculenta, respectively. Comparative analysis of the coat protein of the three DsMV isolates showed the presence of DVG motif (A. paeoniifolius and C. esculenta) and DTG motif in X. sagittifolium and several varying potential threonine and asparagine rich N-glycosylation motifs. Single amino acid substitution of the several conserved motifs occurs in all the three DsMV isolates. This is the first characterization of DsMV isolates infecting A. paeoniifolius, C. esculenta, and X. sagittifolium plants in India.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Babu¹,

Hegde²

2014

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“…Among the various diseases affecting this crop, mosaic disease caused by Dasheen mosaic virus (DsMV) is the most devastating. DsMV belonging to Potyviridae family was first reported in A. paeoniifolius by Pandit et al (2001). The symptoms range from mild mosaic, leaf puckering, yellowing of leaves, shoe string and severe leaf deformation.…”

Section: Introductionmentioning

confidence: 99%

Whole transcriptome sequencing of diseased elephant foot yam reveals complete genome sequence of Dasheen mosaic virus

et al. 2015

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Dasheen mosaic virus (DsMV) the main causative agent of mosaic disease in elephant foot yam (Amorphophallus paeoniifolius) belongs to Potyviridae. The complete genome sequence of DsMV infecting A. paeoniifolius in Kerala state, India was assembled from the whole transcriptome sequencing reads of diseased host samples. The sequence of the virus which showed 83% identity with DsMV infecting Zantedeschia aethiopica (China) was confirmed through amplification and sequencing with primers designed based on the assembled sequence. Unambiguous recombination events mainly confined to the 5′ terminal 4000 nucleotides of the genome with phylogenetically related potyviruses were recognized. The length and genome composition towards the 3′ end comprising NIb, CP and 3′ UTR were not dependent on the host or the source region.

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“…The high incidence of disease and non-availability of quality seed material are the major production constraints (Venkatram et al 2007). Among the various diseases affecting this crop, the production is mainly limited by mosaic disease caused by Dasheen mosaic virus (DsMV), a potyvirus, first reported by Pandit et al (2001). Although many other viruses are supposed to be involved in the mixed infection of the plant, DsMV remains the most substantiated and characterised one (Awasthi et al 2007;Babu et al 2011;Padmavathi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of in vitro lines propagated from corm bud tip of elephant foot yam (Amorphophallus paeoniifolius) approves complete potyviruses elimination

Kamala

Makeshkumar

2015

Eur J Plant Pathol

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The most characterised Dasheen mosaic virus and many other unreported putative viruses are involved in the mixed viral mosaic infection of elephant foot yam (Amorphophallus paeoniifolius). The in vitro propagation of corm bud tips for virus free plantlet production was carried out in three different culture phases consisting of callusing, shoot regeneration and rooting. A 100 % survival rate was recorded on hardening in sand: soil: coir pith (1:1:1) mixture. A total of 84 % of regenerated plantlets were found to be virus free on indexing of 21 in vitro lines with species specific/genus specific serological and molecular diagnostic techniques. Transcriptome sequencing was carried out for two randomly selected in vitro plants and a mosaic infected field sample. Not any of the known potyviruses were traced in the transcriptome profiles of supposed virus free plants thus confirming the complete potyviruses elimination. Disease symptoms or reoccurrence was not observed in the hardened virus-free lines of the plant.

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First report of Dasheen mosaic virus in elephant foot yam in India

Cited by 16 publications

References 2 publications

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Molecular characterization of dasheen mosaic virus isolates infecting edible aroids in India

Whole transcriptome sequencing of diseased elephant foot yam reveals complete genome sequence of Dasheen mosaic virus

Transcriptome profiling of in vitro lines propagated from corm bud tip of elephant foot yam (Amorphophallus paeoniifolius) approves complete potyviruses elimination

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