First Report of <i>Dactylonectria alcacerensis</i> and <i>Dactylonectria torresensis</i> Associated with Black Foot Disease of Grapevine in Turkey

Gungor-Savas, N.; Akgül, Davut Soner; Özarslandan, Mümine; Yildiz, Murat

doi:10.1094/pdis-02-20-0385-pdn

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…alicantinum, this species was originally described from loquat (Eriobotrya japonica) roots in Spain (Agustí-Brisach et al, 2016), and recently it was already reported on grapevines in Turkiye in the year 2020 (Özben, 2020). Savaş et al, (2020) reported D. alcacerensis and D. torresensis for the first time in Turkiye, but we could not find D. alcacerensis in our analysis from the asymptomatic plants. Özben (2020) found six BF pathogens species in symptomatic grapevines sampled from 12 grapevine nurseries in Turkiye, being I. liriodendri and D. macrodidyma (identified with species-specific primers) the most frequent.…”

Section: Discussioncontrasting

confidence: 80%

“…parva from rootstocks of symptomatic grapevines in Ankara and these authors proved its pathogenicity on Sultana Seedless plants in greenhouse conditions. More recently, D. alcacerensis and D. torresensis species were isolated from the roots of 2-3-year-old young vines (SO4 / Chardonnay and 1103 Paulsen / Victoria) in Tekirdağ and Mersin provinces, showing disease incidence rates of 2.5% and 3.0% in the surveyed vineyards, respectively (Savaş et al, 2020). Cylindrodendrum (Cm.)…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and diversity of black-foot pathogens on asymptomatic nursery-produced grapevines in Turkiye

et al. 2022

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Black-foot (BF) disease of grapevines in nurseries and young vineyards is caused by soilborne Cylindrocarpon-like asexual morphs. They can be found both in symptomatic and asymptomatic vines, being spread to new grape growing areas during vineyard establishment. In this study, 42 grapevine nurseries located in different geographical regions in Turkiye were surveyed in 2021 to determine the presence of BF pathogens on asymptomatic marketable plants.Black-foot fungi were isolated from the roots or basal ends of asymptomatic dormant vines in 39 of them (92.9%). The percentage of isolation of BF pathogens ranged from 1.4 to 51.4% (average 18.4%). Seven species: Cylindrodendrum alicantinum, Cylindrocladiella peruviana, Dactylonectria macrodidyma, D. novozelandica, D. torresensis, Ilyonectria liriodendri, and I.2 robusta were identified based on DNA sequencing of histone H3 gene and phylogenetic analyses, D. torresensis being the most frequent. From these species Ca. peruviana, D. novozelandica and I. robusta were detected for the first time on grapevines in Turkiye. Pathogenicity tests on 1103P rootstock cuttings revealed that all species significantly decreased root biomass and increased root disease severity index, when compared with the non-inoculated control, D. novozelandica being the most virulent. Pathogenicity of Cm. alicantinum to grapevine was confirmed for the first time, thus this species should be included as causal agent of BF of grapevines. These findings point out that BF pathogens are highly prevalent in asymptomatic nursery produced grapevines and could represent a serious threat for Turkish viticulture.

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Section: Discussioncontrasting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Occurrence and diversity of black-foot pathogens on asymptomatic nursery-produced grapevines in Turkiye

et al. 2022

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“…Among these, Da. torresensis is the most frequently isolated species [48][49][50][51][52][53], already reported from young vineyards in Spain, Australia, Canada, the Czech Republic, New Zealand, Portugal, South Africa, Spain, and Turkey [49,[51][52][53], and from symptomless grapevine nursery stock in Spain [54]. The growth of the other four tested fungi (D. bohemiae, D. ampelina, Dip.…”

Section: Discussionmentioning

confidence: 99%

Lignans Extract from Knotwood of Norway Spruce—A Possible New Weapon against GTDs

Špetík

Balík

Híc

et al. 2022

JoF

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Grapevine trunk diseases (GTDs) pose a major threat to the wine industry worldwide. Currently, efficient biological methods or chemical compounds are not available for the treatment of infected grapevines. In the present study, we used an extract from the knotwood of spruce trees as a biological control against GTDs. Our in vitro trial was focused on the antifungal effects of the extract against the most common GTD pathogens—Cadophora luteo-olivacea, Dactylonectria torresensis, Diaporthe ampelina, Diaporthe bohemiae, Diplodia seriata, Eutypa latai, and Phaeoacremonium minimum. Our in vitro trial revealed a high antifungal effect of the extract against all tested fungi. The inhibition rates varied among the different species from 30% to 100% using 1 mg·mL−1 extract. Subsequently, the efficiency of the extract was supported by an in planta experiment. Commercial grafts of Vitis vinifera were treated with the extract and planted. The total genomic DNA of grapevines was extracted 10 days and 180 days after the treatment. The fungal microbial diversities of the treated/untreated plants were compared using high-throughput amplicon sequencing (HTAS). Treated plants showed 76.9% lower relative abundance of the genus Diaporthe and 70% lower relative abundance of the genus Phaeoacremonium 10 days after treatment. A similar scenario was observed for the genus Cadophora 180 days after treatment, where treated plants showed 76% lower relative abundance of this genus compared with untreated grapevines.

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Molecular Characterization of Black Foot Disease Pathogens in Grapevine Nurseries and Evaluation of Some Fungicides for Control of the Most Virulent Isolates

Yildiz

TOSUN

2022

Trakya University Journal of Natural Sciences

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Black foot disease, which is mainly caused by Ilyonectria Chaverri & Salgado and Dactylonectria L. Lombard & Crous species, causes serious destruction in most of the wine and grape producing regions of the world, particularly in nurseries and young vineyards. The aim of this research was to evaluate the present situation of black foot disease in grapevine nurseries (Vitis Vinifera L.), to identify its potential agents at morphological and molecular levels and to develop a control strategy against isolates found as the major virulent ones in pathogenicity tests. This study also represents the first attempt to control black foot disease in grapevine saplings in Turkey. Preliminary surveys were carried out in 21 grapevine nurseries in the Aegean Region during 2019 growing season. Black foot species obtained from vine saplings were identified as Ilyonectria liriodendri (Halleen, Rego & Crous), Dactylonectria macrodidyma (Halleen, Schroers & Crous) and D. torresensis (A. Cabral, Rego & Crous), using morphological and molecular methods. The pathogenicity studies revealed that the most virulent isolates belonged to I. liriodendri. Twelve chemical and one biological fungicides were screened in vitro for mycelial inhibition of I. liriodendri isolates. The experiments were performed in three replicates for each of the chemical fungicides. Sterile distilled water, instead of fungicide solution, was used for control trials. Mixtures of cyprodinil + fludioxonil, thiabendazole + fludioxonil + metalaxyl-m + azoxystrobin and azoxystrobin + metalaxyl-m + fludioxonil were the most effective chemical fungicides tested in mycelial inhibition of I. liriodendri isolates. Chemical fungicides mixtures of boscalid + kresoxim-methyl, promocarb hydrochloride (HCL) + fosetyl-al, azoxystrobin + difenoconazole, fludioxonil + metalaxyl-m and individual fungicides; cyprodinil, fluoxapyroxad, hymexazole, fenhexamid and 8-hydroxyquinoline sulphate were not effective on mycelial inhibition. Biocontrol activity of Trichoderma harzianum Rifai KRL-AG2 strain against I. liriodendri was evaluated. The experiment was performed in four replicates for each isolate. Petri dishes with only I. liriodendri mycelial discs used as controls. Treatment with Trichoderma harzianum Rifai KRL-AG2 strain provided 60.1% to 80.6% inhibition against I. liriodendri isolates. The most effective fungicides tested in vitro were evaluated in 2020 and 2021 under controlled conditions against possible infections of I. liriodendri during the rooting process of 1103 Paulsen grapevine rootstocks. Mixtures of thiabendazol + fludioxonil + metalaxyl-m + azoxystrobin and cyprodinil + fludioxonil were the most effective fungicides in the experiments conducted in 2020 and 2021, followed by azoxystrobin + metalaxyl-m + fludioxonil and Trichoderma harzianum Rifai KRL-AG2 strain. Özet: Esas olarak Ilyonectria Chaverri & Salgado ve Dactylonectria L. Lombard & Crous türlerinin neden olduğu karabacak hastalığı, dünyanın şarap ve üzüm üreten bölgelerinin çoğunda, özellikle fidanlıklarda ve gen...

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First Report of Dactylonectria alcacerensis and Dactylonectria torresensis Associated with Black Foot Disease of Grapevine in Turkey

Cited by 3 publications

References 5 publications

Occurrence and diversity of black-foot pathogens on asymptomatic nursery-produced grapevines in Turkiye

Occurrence and diversity of black-foot pathogens on asymptomatic nursery-produced grapevines in Turkiye

Lignans Extract from Knotwood of Norway Spruce—A Possible New Weapon against GTDs

Molecular Characterization of Black Foot Disease Pathogens in Grapevine Nurseries and Evaluation of Some Fungicides for Control of the Most Virulent Isolates

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