First Report of Cercospora cf. flagellaris on Industrial Hemp (Cannabis sativa) in Kentucky

Doyle, Vinson P.; Tonry, H. T.; Amsden, B.; Beale, Julie; Dixon, Ed; Li, H.; Szarka, Desiree; Gauthier, Nicole Ward

doi:10.1094/pdis-01-19-0135-pdn

Cited by 11 publications

(7 citation statements)

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“…An increasing number of new reports also identify Golovinomyces spadiceus powdery mildew and Cercospora cf. flagellaris leaf spot on industrial Cannabis in the US [103,104], suggesting the importance of employing more advanced high-throughput technologies in Cannabis research. Rare metagenomic and qPCR studies [102] in medical Cannabis literature reveal the existence of additional pathogenic or toxigenic bacterial and fungal species.…”

Section: Where Do the Scientific Solutions Lie?mentioning

confidence: 99%

Scientific Prospects for Cannabis-Microbiome Research to Ensure Quality and Safety of Products

et al. 2020

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Cannabis legalization has occurred in several countries worldwide. Along with steadily growing research in Cannabis healthcare science, there is an increasing interest for scientific-based knowledge in plant microbiology and food science, with work connecting the plant microbiome and plant health to product quality across the value chain of cannabis. This review paper provides an overview of the state of knowledge and challenges in Cannabis science, and thereby identifies critical risk management and safety issues in order to capitalize on innovations while ensuring product quality control. It highlights scientific gap areas to steer future research, with an emphasis on plant-microbiome sciences committed to using cutting-edge technologies for more efficient Cannabis production and high-quality products intended for recreational, pharmaceutical, and medicinal use.

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Section: Where Do the Scientific Solutions Lie?mentioning

confidence: 99%

Scientific Prospects for Cannabis-Microbiome Research to Ensure Quality and Safety of Products

et al. 2020

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“…Aside from the major fungal genera mentioned above that have been identified as causal agents of significant diseases in bast fiber crops, other fungal pathogens, such as Cercospora cf. flagellaris [48], Exserohilum rostratum [49], Macrophomina phaseolina [50], Sclerotinia minor [51], Micropeltopsis cannabis sp., Orbilia luteola, Curvularia cymbopogonis [52], Podosphaera xanthii [53], and Lasiodiplodia theobromae [54,55], can also cause a diversity of known or unknown diseases among bast fiber crops. The diseases include leaf spot, foliar blight, charcoal rot, sclerotinia crown rot in industrial hemp, powdery mildew on ramie, and black rot on kenaf, resulting in different degrees of damage to productivity and quality [48][49][50][51][52][53][54][55].…”

Section: Fungal Pathogens Of Bast Fiber Cropsmentioning

confidence: 99%

“…Thus, it is common nowadays to combine ITS sequence information with those from other gene fragments. Several genes have been commonly used, including those coding for translation elongation factor 1-α (TEF1-α or EF-1α) [32], ß-tubulin (TUB) [42], calmodulin (CAL) [50], histone subunit 3 (H3) [48], glyceraldehydes-3-phosphate dehydrogenase (GAPDH) [47], and actin (ACT) [48]. By sequencing the ITS and 28S regions with primers PM5G/NLP2, Szarka et al [43] made the first documented report of G. spadiceus causing powdery mildew on industrial hemp in the United States [43].…”

Section: Target Dna Selection and Molecular Assays Of Fungal Pathogenmentioning

confidence: 99%

“…As a result, polymorphisms from all gene fragments can be used together to allow for greater discrimination of strains and species than single gene sequences [71][72][73]. This approach has been used by Doyle et al [48] in their analysis of fungal pathogens causing leaf spot diseases in industrial hemp fields in Kentucky in 2015. Specifically, they obtained sequences for parts of CAL, H3, ACT, and EF1-α genes and the complete ITS sequences for their strains and identified Cercospora cf.…”

Section: Target Dna Selection and Molecular Assays Of Fungal Pathogenmentioning

confidence: 99%

“…Specifically, they obtained sequences for parts of CAL, H3, ACT, and EF1-α genes and the complete ITS sequences for their strains and identified Cercospora cf. flagellaris as the causal agent of leaf spot disease in their fields [48]. Similarly, based on sequences at six loci (ACT, TUB2, CAL, GS, GAPDH, and ITS), Niu et al revealed that C. fructicola, C. siamense, and C. corchorumcapsularis sp.…”

Section: Target Dna Selection and Molecular Assays Of Fungal Pathogenmentioning

confidence: 99%

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Molecular Diagnostics and Pathogenesis of Fungal Pathogens on Bast Fiber Crops

et al. 2020

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Bast fibers and products derived from them are undergoing a resurgence in demand in the global market. However, fungal diseases have become an important factor limiting their yield and quality, causing devastating consequences for the production of bast fiber crops in many parts of the world. Thus, there is a high demand for effective control and prevention strategies against fungal pathogens. Having rapid, specific, sensitive, and cost-effective tests that can be used for early and accurate diagnosis of disease agents is an essential step of such strategies. The objective of this study was to review the current status of research on molecular diagnosis of fungal pathogens on bast fiber crops. Our search of PubMed identified nearly 20 genera of fungal pathogens on bast fiber crops, among which the five most common genera were Colletotrichum, Pythium, Verticillium, Fusarium, and Golovinomyces. The gene regions that have been used for molecular identifications of these fungi include internal transcribed spacer (ITS), translation elongation factor 1-α (EF-1α), ß-tubulin, calmodulin (CAL), histone subunit 3 (H3), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), etc. We summarize the molecular assays that have been used to identify these fungi and discuss potential areas of future development for fast, specific, and accurate diagnosis of fungal pathogens on bast fiber crops.Pathogens 2020, 9, 223 2 of 19 and control. However, conventional protocols based on morphological and physiological methods are time-consuming, require significant experience, and may not be sensitive and specific for individual pathogens [3]. Moreover, many fungal pathogens can remain latent in "sub-infection" stages with no obvious symptoms and/or in low numbers, making them difficult to detect, and causing confusion with their roles in diseases. These issues can contribute to delayed or wrong control measures.During the last three decades, to overcome these problems and minimize crop losses caused by fungal diseases, a diversity of DNA molecule-based tools has been developed for the detection and identification of fungal pathogens. These techniques include conventional polymerase chain reaction (PCR) [4], quantitative PCR (qPCR) [5,6], immunocapture-PCR (IC-PCR) [7,8], droplet digital PCR (dd-PCR) [9], loop-mediated isothermal amplification (LAMP) [10], multiplex tandem PCR [11], fluorescence in situ hybridization (FISH) [12], and DNA microarrays [3]. These methods are typically faster and more accurate than those based on colony morphology, microscopic features, and/or physiological/biochemical characters of pure fungal cultures. Indeed, methods targeting DNA sequences have been applied to detect pathogens during crops' growth, harvest and postharvest processing stages [13]. Moreover, they have also enabled a deeper understanding of microbial populations and communities associated with crops, especially the microorganisms that are difficult or impossible to cultivate in the lab. Together, technological advances and developments in DNA molecu...

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Pathogenicity of seedborne Alternaria and Stemphylium species and stem-infecting Neofusicoccum and Lasiodiplodia species to cannabis (Cannabis sativa L., marijuana) plants

Roberts

Punja

2021

Canadian Journal of Plant Pathology

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First Report of Cercospora cf. flagellaris on Industrial Hemp (Cannabis sativa) in Kentucky

Cited by 11 publications

References 3 publications

Scientific Prospects for Cannabis-Microbiome Research to Ensure Quality and Safety of Products

Scientific Prospects for Cannabis-Microbiome Research to Ensure Quality and Safety of Products

Molecular Diagnostics and Pathogenesis of Fungal Pathogens on Bast Fiber Crops

Pathogenicity of seedborne Alternaria and Stemphylium species and stem-infecting Neofusicoccum and Lasiodiplodia species to cannabis (Cannabis sativa L., marijuana) plants

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