First report of <i>Ceratocystis fimbriata</i> causing fruit‐rot of <i>Passiflora edulis</i> in Brazil

Firmino, Ana Carolina; Novaes, Quelmo Silva de; Tozze, Hugo José; Sobrinho, Geraldo Gomes Rocha; Santos, Armínio; Bezerra, José Luiz; Furtado, E. L.

doi:10.5197/j.2044-0588.2013.027.004

Cited by 4 publications

(3 citation statements)

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“…Ceratocystis fimbriata occurs commonly on eucalyptus and mango in Brazil and has been reported recently on G. arborea (Luchi et al , 2013) and Passiflora edulis (Firmino et al , 2013). There is also a report of a Ceratocystis sp.…”

Section: Figurementioning

confidence: 91%

First report of Ceratocystis fimbriata causing wilt on Khaya senegalensis

Firmino

Matos

Tozze

et al. 2017

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Brazil has an active forestry sector, including production for both wood pulp and timber. The species Khaya senegalensis (African mahogany) has been introduced into the country because it produces good quality timber and its wood is valued for furniture making. It is estimated that more than 100,000 ha of African mahogany has been planted in Brazil. In July 2016, about 25 seven-year-old K. senegalensis trees in an area of approximately 1 ha were found with wilt symptoms in the region of Gloria de Dourados, Mato Grosso State, Brazil.Stem fragments, approximately 1 cm 2 in size, with characteristic symptoms were collected, surface-sterilised with 1% NaOCl for 1 min, rinsed twice in sterilised distilled water, and placed on carrot baits (Moller & DeVay, 1968). The carrot disks were taped together and incubated in a moist chamber at 25 ±2°C for one week. After sporulation of the fungus on the carrot tissue, the ascospore masses formed at the tip of the perithecia were transferred to malt extract agar (MEA). After 10 days, black, globose perithecia (137.1 × 133.52 µm) with a long rostrum (577.43 µm) were observed (Fig. 1). Ascospores were hat-shaped, measuring 3.98 × 5.26 µm. Asexual aleurioconidia were brown (12.8 x 8.94 µm) and two types of endoconidia were observed: cylindrical (21.18 × 4.77 µm) and doliiform (9.07 × 8.23 µm), in chains (Figs. 2-3). The morphological characteristics indicated that the fungal isolate was of the genus Ceratocystis. DNA from mycelium grown on MEA was collected, extracted and used to amplify the ITS-5.8S rDNA region based on the protocol described by Johnson et al. (2005). The ITS sequence of the nuclear rDNA (GenBank Accession No. MF280402) was 99% identical to that of C. fimbriata isolate CBS115173 (KC493168) isolated from Gmelina arborea in Brazil (Luchi et al., 2013).Inoculation of plants was performed using a method modified from Silveira et al. (2006). One-centimetre mycelial discs of the fungus (from ten-dayold colonies on MEA) were placed onto injured stems of six-month-old healthy K. senegalensis saplings. The inoculation site was wrapped with cotton cloth (moistened with sterile distilled water) and plastic film. Plants were inoculated with a sterile MEA disc as a negative control. Ten plants were inoculated for each treatment. The inoculated plants were kept in the greenhouse with an average temperature of 30°C. Wilt symptoms caused by C. fimbriata were observed 60 days after inoculation and plant death at 90 days (Fig. 4). The fungus was re-isolated in culture from the stem of the inoculated African mahogany, confirming pathogenicity. Control plants were symptomless.

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Section: Figurementioning

confidence: 91%

First report of Ceratocystis fimbriata causing wilt on Khaya senegalensis

Firmino

Matos

Tozze

et al. 2017

New Disease Reports

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“…These fruits had perithecia typical of Ceratocystis, which was isolated and deposited in the mycology collection located at the Laboratory of Forest Pathology, of the School of Agronomical Sciences of Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), in Botucatu, São Paulo, Brazil (Firmino et al, 2013). For its molecular characterization, the isolate was recovered, removed from the oil and cultured on PDA medium (potato, dextrose and agar) for DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

“…Currently, this fungus has been shown harmful to other cultures like cacao (Theobroma cacao) (Bezerra, 1997), fig (Ficus carica) (Valarini;Tokeshi, 1980), teak (Tectona grandis) (Firmino et al, 2012b), atemoya (hybrid of Annona cherimola and Annona squamosa) (Firmino et al, 2012a), and, recently, yellow passion fruit (Passiflora edulis f. flavicarpa) (Firmino et al, 2013). In this last case, the fungus causes rot to the fruits without causing any damage to the plant.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Ceratocystis fimbriata from passion fruits

et al. 2016

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RESUMO:Frutos de maracujá (Passiflora edulis) foram encontrados com sintomas de podridão no campo, na cidade de Tanhaçu, Bahia. Após isolamento do patógeno associado a essas podridões, o presente trabalho teve como objetivo realizar a caracterização de um isolado de Ceratocystis de maracujá para melhor compreender esse patossistema. A caracterização molecular foi realizada com base no sequenciamento da região ITS-5.8S rDNA. Realizou-se a caracterização patogênica em mudas e frutos de maracujá. A colonização dos frutos de maracujá foi acompanhada com técnicas de microscopia de varredura (MEV). A análise do DNA do isolado de Ceratocystis mostrou que este pertence à espécie Ceratocystis fimbriata. As mudas de maracujá inoculadas apresentaram lesão 30 dias após a inoculação, e não foram observadas murcha nem morte das plantas inoculadas. Nos frutos não foram constatadas diferenças nas lesões causadas por esse fungo entre os cultivares, com médias de lesões de 1,0 a 2,2 cm de diâmetro, aos 7 e 11 dias, respectivamente. Nas análises realizadas em MEV, foram observadas a germinação dos esporos e a penetração do fungo nos frutos no período entre 2 e 6 horas após a inoculação. Doze e 24 horas após a inoculação foi visualizada a colonização do fruto, tanto externa como internamente, e 48 horas após a inoculação se notou o início da degradação da parede externa da casca do fruto. Noventa horas após a inoculação, observou-se a formação de novos peritécios, tanto na parte interna como na parte externa do fruto. Este estudo vem complementar as informações relacionadas à interação desse fungo com frutos de maracujazeiro. PALAVRAS-CHAVE: podridão; espécies; microscopia.ABSTRACT: Passion fruits (Passiflora edulis) were found with symptoms of rot in the field, in the city of Tanhaçu, Bahia. After isolating the pathogen associated with this rot, in the present study we aimed to characterize the Ceratocystis isolate from passion fruit for better understanding this pathosystem. Molecular characterization was done based on the region ITS-5.8S rDNA. Pathogenic characterization was carried out for seedlings and fruits of passionflower. Passion fruit colonization was monitored by means of scanning electron microscopy techniques (SEM). DNA analysis of the Ceratocystis isolate from passionflower pointed out that this species belongs to Ceratocystis fimbriata. The inoculated passionflower seedlings showed injury at 30 days post-inoculation, but no inoculated plant showed wilt or died. Considering fruits, no differences were found for lesions caused by this fungus among cultivars, and lesions had average diameters of 1.0 and 2.2 cm at 7 and 11 days, respectively. The analysis using SEM indicated fungus spore germination and penetration in the fruit between 2 and 6 hours post-inoculation. At 12 and 24 hours post-inoculation, fruit colonization was noted both externally and internally, while fruit wall degradation started at 48 hours post-inoculation. At 90 hours post-inoculation, formation of new perithecia was observed inside and outside th...

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