First Report of <i>Cassava common mosaic virus</i> and Cassava frogskin-associated virus Infecting Cassava in Argentina

Feo, L. Di; Aa, Zanini; Pardina, Patricia Rodríguez; Cuervo, Maritza; Carvajal-Yepes, Mónica; Cuellar, Wilmer J.

doi:10.1094/pdis-10-14-1088-pdn

Cited by 14 publications

(7 citation statements)

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“…In Argentina, at least three viruses have been reported (Zanini et al ., ; Di Feo et al ., ). Cassava common mosaic virus (CsCMV) is a re‐emergent virus significantly affecting cassava production in South America; it has been reported in Venezuela (Chaparro‐Martinez & Trujillo‐Pinto, ), Peru (Costa & Kitajima, ; Fernandez et al ., ), Colombia, Brazil and Paraguay (Silva et al ., ; Calvert et al ., ; Lozano et al ., ) and recently in Argentina (Di Feo et al ., ), close to the Paraná region where it was previously reported. The affected plants develop mosaic and chlorosis in leaves, with increased severity in the subtropical zones of South America due to the prolonged cold periods (Calvert et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cuellar

Celli

et al. 2018

Plant Pathology

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Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT‐PCR and sequencing. In this work, the virus has been successfully purified and a double‐antibody sandwich (DAS‐) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co‐infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.

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Section: Introductionmentioning

confidence: 97%

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cuellar

Celli

et al. 2018

Plant Pathology

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“…CsCMV was first reported in the south of Brazil (Costa, 1940; Silberschmid, 1938) and later in Colombia, Paraguay (Carvajal‐Yepes et al, 2014; Lozano et al, 2017; Nolt et al, 1991), Venezuela (Lozano et al, 2017; Marys & Izaguirre‐Mayoral, 1995; Mejías et al, 2015), Mexico (Elliott & Zettler, 1987; Lozano et al, 2017; Zettler, 1986), and Peru (Fernandez et al, 2017). In Argentina, the importance of the virus increased when its presence, in single and co‐infections with other viral species, was confirmed in the main cassava‐producing areas with 100% prevalence (Di Feo et al, 2015; Zanini et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Cassava common mosaic virus infection affects growth and yield components of cassava plants (Manihot esculenta) in Argentina

Collavino

Medina

et al. 2021

Plant Pathology

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Cassava is an important crop with great economic and social significance in many countries. Most of its biomass is usable: storage roots can be destined for industry or fresh consumption, leaves are a source of protein, vitamins, and minerals, and stems can be used as propagation material. Due to its vegetative propagation, cassava yield is greatly constrained by viral diseases. Cassava common mosaic virus (CsCMV), one of the most widely spread viruses in Latin America, is the only cassava‐infecting potexvirus able to cause disease in a single infection: cassava common mosaic disease (CCMD). Here, we evaluated the effect of an Argentinian strain of CsCMV on several agronomic traits. Field trials were established in north‐eastern Argentina for three successive cropping seasons, 2016/17, 2017/18, and 2018/19. CsCMV presence was confirmed by PCR or ELISA tests in mechanically inoculated cassava plants. The evaluated traits were plant height (PH), total fresh weight (TFW), shoot fresh weight (SFW), diameter and FW of tuberous root per plant (TRD and FWTR), FW of each individual tuberous root (FWiTR), length and number of tuberous roots (TRL and TRN), harvest index (HI), and estimated yield of tuberous root (EY) in both infected and noninfected plants. Significant yield losses were recorded in infected plants. The most affected traits were EY and FWTR (both showing 44.3% of losses), followed by TFW, SFW, TRN, and FWiTR (37.8%, 33.5%, 29.9%, and 24.8% of losses, respectively). These losses are extremely high for Argentina, where this crop provides food security and demand for the raw material is unsatisfied.

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“…RT-PCR : Cassava common mosaic virus (CsCMV; Fam. Alphaflexiviridae ) is a re-emergent RNA virus infecting cassava in the Americas [ 4 , 6 , 8 ]. We have collaborated with research groups in Peru, Argentina and Colombia to detect and characterize the virus using the described protocol [ 13 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)

et al. 2021

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Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue. DNA purity estimations reveal OD260/280 ratios above 2.0 and OD260/230 ratios above 1.7, even for samples stored in silica gel for several months. The high quality of the extracts is suitable for detection of DNA and RNA viruses, with high efficiency. We suggest this method could be used as part of a gold standard kit for virus detection in cassava.

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First Report of Cassava common mosaic virus and Cassava frogskin-associated virus Infecting Cassava in Argentina

Cited by 14 publications

References 4 publications

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cassava common mosaic virus infection affects growth and yield components of cassava plants (Manihot esculenta) in Argentina

An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)

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