First report of ‘<i>Candidatus</i> Phytoplasma asteris’ (16SrI group) causing stunt of tomato in Cuba

Zamora, Loidy; Acosta, K.; Piñol, B.; Quiñones, Madelaine; Bertaccini, Assunta

doi:10.5197/j.2044-0588.2014.030.010

Cited by 8 publications

(4 citation statements)

References 5 publications

(5 reference statements)

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“…These phytoplasmas were already reported in the Caribbean areas and in some cases also in citrus crops (Teixeira et al 2009;Caicedo et al 2015, Mauricio-Castillo et al 2015. In Cuba the presence of phytoplasmas in the 16SrI group was reported in papaya, beans, peppers, cabbage, beets, carrots, cassava, soybeans, strawberry and macadamia, 16SrII group in papaya, radish and medlar, and the 16SrX group in papaya (Arocha et al 2007;Arocha et al 2009;Zamora et al 2012Zamora et al , 2014Acosta-Pérez et al 2013;Ferriol-Marchena et al 2013;Pérez-López et al 2013). All these species are cultivated as intercrop in citrus fields or are planted closely to citrus orchards.…”

Section: Resultsmentioning

confidence: 60%

‘Candidatus Phytoplasma’ and ‘Candidatus Liberibacter’ species detection in citrus

Bertaccini¹,

Satta²,

Luis-Pantoja³

et al. 2019

Phyt. Moll.

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Huanglongbing" (HLB) is one of the most devastating diseases of citrus orchards worldwide. Samples from 183 citrus plants of different cultivars and rootstock/cultivar combinations, showing HLB symptoms in three Caribbean countries (Cuba, Jamaica, and Guadeloupe-France), were collected to verify the possible co-infection of 'Candidatus Phytoplasma' and 'Candidatus Liberibacter' species. The 64% of the samples resulted positive to the 'Ca. L. asiaticus' and the 27% to diverse 'Ca. Phytoplasma'-related species, moreover about the 14% of the samples infected with 'Ca. Liberibacter' were also found positive to phytoplasmas, indicating the presence of mixed infection especially in the orchards located in Cuba. Moreover, in one of the samples from Jamaica mixed phytoplasma infection was detected. Moreover the detection of only phytoplasmas in 11 symptomatic citrus samples collected from Cuba and Guadeloupe without 'Ca. Liberibacter' detection, confirmed that the symptomatology cannot be the sole criterium to discriminate between the presence of the two pathogens, and molecular detection is necessary to identify single or mixed infections. Diaphorina citri insects collected from Cuba and Guadeloupe resulted infected with 'Ca. L. asiaticus' confirming its active role in the dissemination of the pathogen. Only one insect of the Cicadidae family, collected in Guadeloupe, was found positive for phytoplasma presence. Considering that the phytoplasmas belonging to some 'Candidatus species' were detected in the three countries in different citrus varieties, a relevant role as phytoplasma reservoir can be attribute to citrus orchards.

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Section: Resultsmentioning

confidence: 60%

‘Candidatus Phytoplasma’ and ‘Candidatus Liberibacter’ species detection in citrus

Bertaccini¹,

Satta²,

Luis-Pantoja³

et al. 2019

Phyt. Moll.

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“…Investigations based on PCR detection using universal primers specific to phytoplasma confirmed its association in 25% of analyzed plants that showed disease symptoms, indicating expansion of the host range of Ca. P. asteris, in addition to the already reported expansion to host plants, Luffa and cotton [39], brinjal [40], sandalwood and Withania somnifera in India, Cucumis sativus L. in Taiwan, carrot, cabbage, beetroot and tomato in Cuba [1,41,42] and Sechium edule (Jacquin) Swartz in Costa Rica [43].…”

Section: Discussionmentioning

confidence: 80%

“…In addition, virtual RFLP analysis was conducted for 11 more enzymes. It revealed differences only for the MseI RFLP pattern (a similarity coefficient of 0.91) and 16Sr I phytoplasma associated with brinjal littleleaf, sandal spike and Withania somnifera phytoplasma from India [1,41,42].…”

Section: Discussionmentioning

confidence: 96%

Detection and characterization of ‘Candidatus Phytoplasma asteris’ associated with littleleaf disease of bitter gourd from India by 16S rRNA phylogenetic and RFLP (in vitro and virtual) analysis

Venkataravanappa¹,

Reddy²,

Swarnalatha³

et al. 2017

Arch biol sci (Beogr)

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Bitter gourd plants showing symptoms of little leaf disease are prevalent in farmers' fields in the Bangalore rural district, Karnataka state, India. Twenty leaf samples from different locations were collected to determine the etiology of the disease. Using PCR and phytoplasma 16S rRNA gene-specific universal primers, we observed positive amplification for the phytoplasma specific primers in five out of twenty samples. The amplified products were cloned, sequenced and nucleotide (NT) sequence comparisons were made with the available phytoplasmas' 16S rRNA gene NT sequences in the NCBI database. The 16S rRNA gene NT sequence of bitter gourd phytoplasma shared highest identity of 81.7-96.0% with 'Candidatus Phytoplasma asteris' (Ca. P. asteris) 16Sr I group isolates from different parts of the world. This was supported by close clustering of phytoplasma of the current study with the Ca. P. asteris 16Sr I subgroup by phylogenetic analysis. The virtual restriction fragment length polymorphism (RFLP) pattern generated for the Phytoplasma from bitter gourd was in congruence with the in vitro RFLP pattern for the six enzymes. This was typical to Ca. P. asteris from the 16Sr I group. Further, virtual RFLP analysis with 11 more enzymes used for RFLP pattern prediction revealed differences only in the Mse I RFLP pattern, with a similarity coefficient of 0.91, which is less than the threshold similarity coefficient for a new subgroup. We propose that the phytoplasma detected in the present study that infects bitter gourd and causes littleleaf disease should be considered as a new subgroup of group 16Sr I (Ca. P. asteris). This is the first report of phytoplasma associated with littleleaf disease of bitter gourd from India.

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“…The principal phytoplasma groups infecting tomato are in group 16SrI, 16SrII, 16SrIII, 16SrV, and 16SrVI (Zamora et al, 2014;Singh et al, 2012;Amaral-Mello et al, 2006;Del Serrone et al, 2001;Anfoka et al, 2003), which induce mostly similar symptoms on their respective host (Krawczyk et al, 2010;Pracros et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey

Usta

Güller

2020

Turkish JAF Sci.Tech.

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The tomato plant exhibiting leaf rolling, witches’ broom, distorted and elongated ﬂower’s sepals in Iğdır province, Turkey, was observed. Total DNA extraction was performed from the symtomatic fresh tomato sample. All DNAs were subjected to Direct and Nested polymerase chain reaction (PCR) with universal primer sets that amplified the 16S rRNA of phytoplasmas. PCR products were purified from agarose gel and cloned into the pGEM T-Easy cloning vector. Recombinant plasmids were introduced into the prokaryotic cloning bacteria by electroporation. Plasmid isolation was performed by selecting one of the positive clones randomly and sequencing was performed by Next Generation Sequencing (NGS). Sequencing results revealed that the 16S rRNA gene associated with phytoplasma was 1251 nucleotides in size, and this sequence was denominated as ‘Iğdır 10’ isolates and recorded in the GenBank under the MT344968 accession number. The virtual restriction fragment length polymorphism (V-RFLP) and phylogenetic analysis of the 16S rDNA sequence confirmed that the cause of disease in infected tomato plants was 'Candidatus Phytoplasma trifolii' ('Ca. P. trifolii') (16SrVI-A, Clover proliferation group), with a 1.00 similarity coefficient. This present study is the first report of ‘Ca. P. trifolii’ and its nucleotide sequence analysis in naturally infected tomato in Iğdır province.

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First report of ‘Candidatus Phytoplasma asteris’ (16SrI group) causing stunt of tomato in Cuba

Cited by 8 publications

References 5 publications

‘Candidatus Phytoplasma’ and ‘Candidatus Liberibacter’ species detection in citrus

‘Candidatus Phytoplasma’ and ‘Candidatus Liberibacter’ species detection in citrus

Detection and characterization of ‘Candidatus Phytoplasma asteris’ associated with littleleaf disease of bitter gourd from India by 16S rRNA phylogenetic and RFLP (in vitro and virtual) analysis

Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey

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