First report of Bitylenchus ventrosignatus (Tobar Jiménez, 1969) Siddiqi, 1986 associated with wild grass in Botswana

Abstract

During a survey on the biodiversity of plant-parasitic nematodes of natural areas in Botswana, Bitylenchus ventrosignatus was discovered around the rhizosphere of wild grass. The nematodes were extracted using the tray method and then fixed according to the available protocols. The morphological characters fit well with the description of B. ventrosignatus. In addition, molecular analysis using 18 S and 28 S rDNA indicated 98% (KJ461617) and 95% (KJ461567) similarity with the Spanish population of B. ventrosignatus. The phylogenetic analysis of 18 S and 28 S rDNA placed the examined population with other populations of B. ventrosignatus in a group with a posterior probability support value of 100. According to published literature, this is the first report of B. ventrosignatus from Botswana.

Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran

“… Hosseinvand et al (2020) , indicated that Bitylenchus species divide into two groups. Similar results were obtained by Shokoohi (2021 ). The molecular data of the other known species of Bitylenchus and Tylenchorhynchus will shed light on the phylogenetic relationships of their species and the closely related genera.…”

First report of Tripylina zhejiangensis associated with grassland in South Africa

“…Thirty microliters of 5 % Chelex® 50 and 2 μL of proteinase K was added to the microcentrifuge tube that contained the crushed nematodes and mixed. The microcentrifuge tube with the nematode lysate was incubated at 56 °C for two hours and then incubated at 95 °C for 10 minutes to deactivate the proteinase K and finally spun for 2 min at 16000 rpm ( Shokoohi, 2021 ). The supernatant was then extracted from the tube and stored at –20 °C.…”

First report of Aphelenchoides bicaudatus (Imamura, 1931) Filipjev and Schuurmans Stekhoven, 1941 associated with grass in South Africa

“…Specimens were collected at the Kirstenbosch National Botanical Garden in Cape Town (S: 33° 59’ 13.19”; E 18° 25’ 29.39”) and Magoebaskloof (S: 23°52’40.368”; E: 29°56’14.459”) from the rhizosphere of grass plants (Family: Poaceae; Pennisetum clandestinum ). The specimens were extracted using the tray method ( Shokoohi, 2021 ) and were fixed with a hot 4 % formaldehyde solution and transferred to anhydrous glycerin using the De Grisse (1969 ) method. The classification provided by Handoo et al (2020 ) was used for the taxonomical study of Aphelenchoides .…”

“…Thirty microliters of 5 % Chelex® 50 and 2 μL of proteinase K were added to the microcentrifuge tube that contained the crushed nematodes and mixed. These separate microcentrifuge tubes with the nematode lysate were incubated at 56 °C for two hours and then incubated at 95 °C for 10 minutes to deactivate the proteinase K and finally spin for 2 min at 16000 rpm ( Shokoohi, 2021 ). The supernatant was then extracted from the tube and stored at –20 °C.…”