First Report of <i>Bean common mosaic virus</i> in <i>Cudrania tricuspidata</i> in Korea

Seo, Jang‐Kyun; Kang, Mi-Hyung; Shin, Ohkyu; Kwak, Hae-Ryun; Kim, M.-K.; Choi, Hongsoo; Ko, Sug-Ju

doi:10.1094/pdis-07-14-0678-pdn

Cited by 6 publications

(4 citation statements)

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“…With the extensive investigation of plant viruses and the widespread use of high-throughput sequencing technology, more and more hosts outside the leguminous plants have been found to be natural hosts of BCMV. According to the published literature, there are currently at least four plant species outside the family Leguminosae found to be the natural host of BCMV, including Cudrania tricuspidata ( Seo et al, 2015 ), Alpinia purpurata ( Larrea-Sarmiento et al, 2020 ), Nandina domestica ( Su et al, 2022 ), and Populus alba ( Wang et al, 2023 ). Among them, only BCMV identified in Cudrania tricuspidata and Nandina domestica are reported with complete genome sequence.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the hosts of BCMV are generally thought to be restricted to leguminous plants ( Sáiz et al, 1994 ; Wang et al, 2023 ). With the rapid development of high-throughput sequencing technology, more and more plants outside legume species have been reported as novel hosts of BCMV, including Cudrania tricuspidata ( Seo et al, 2015 ), Alpinia purpurata ( Larrea-Sarmiento et al, 2020 ), and Nandina domestica ( Su et al, 2022 ).…”

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confidence: 99%

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Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing

Xu,

Weng,

Chen

et al. 2024

Plant Pathol J

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Gardenia (Gardenia jasminoides) is a popular and economically vital plant known for its ornamental and medicinal properties. Despite its widespread cultivation, there has been no documentation of plant viruses on gardenia yet. In the present study, gardenia leaves exhibiting symptoms of plant viral diseases were sampled and sequenced by both metatranscriptome and small RNA sequencing. As a consequence, bean common mosaic virus (BCMV) was identified in gardenia for the first time and named BCMV-gardenia. The full genome sequence of BCMV-gardenia is 10,054 nucleotides (nt) in length (excluding the poly (A) at the 3′ termini), encoding a large polyprotein of 3,222 amino acids. Sequence analysis showed that the N-termini of the polyprotein encoded by BCMV-gardenia is less conserved when compared to other BCMV isolates, whereas the C-termini is the most conserved. Maximum likelihood phylogenetic analysis showed that BCMV-gardenia was clustered closely with other BCMV isolates identified outside the leguminous plants. Our results indicated that the majority of BCMV-gardenia virus-derived small interfering RNAs (vsiRNAs) were 21 nt and 22 nt, with 21 nt being more abundant. The first nucleotide at the 5′ termini of vsiRNAs derived from BCMV-gardenia preferred U and A. The ratio of vsiRNAs derived from sense (51.1%) and antisense (48.9%) strands is approaching, and the distribution of vsiRNAs along the viral genome is generally even, with some hot spots forming in local regions. Our findings could provide new insights into the diversity, evolution, and host expansion of BCMV and contribute to the prevention and treatment of this virus.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing

Xu,

Weng,

Chen

et al. 2024

Plant Pathol J

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“…Bean common mosaic virus (BCMV) is one of the most common and most destructive viruses in the genus Potyvirus , the largest virus genus of land plants, and it infects common beans ( Phaseolus vulgaris L.) as well as a range of other cultivated and wild legumes ( Morales et al., 2003 ), and yielded losses could be as high as 50-100% of the production ( Damayanti et al., 2008 ; Saqib et al., 2010 ; Verma and Gupta, 2010 ). Although some other plants, such as Cudrania tricuspidate , were reported as novel hosts of BCMV ( Seo et al., 2015 ), BCMV is known to have a restricted host range outside legume species ( Sáiz et al., 1994 ). In July 2020, a novel poplar yellow−green mosaic disease was found in a P. alba var.…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptome and microRNA sequencing analyses reveal gene responses in poplar leaves infected by the novel pathogen bean common mosaic virus (BCMV)

et al. 2023

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Recently, a novel poplar mosaic disease caused by bean common mosaic virus (BCMV) was investigated in Populus alba var. pyramidalis in China. Symptom characteristics, physiological performance of the host, histopathology, genome sequences and vectors, and gene regulation at the transcriptional and posttranscriptional levels were analyzed and RT−qPCR (quantitative reverse transcription PCR) validation of expression was performed in our experiments. In this work, the mechanisms by which the BCMV pathogen impacts physiological performance and the molecular mechanisms of the poplar response to viral infection were reported. The results showed that BCMV infection decreased the chlorophyll content, inhibited the net photosynthesis rate (Pn) and stomatal conductance (Gs), and significantly changed chlorophyll fluorescence parameters in diseased leaves. Transcriptome analysis revealed that the expression of the majority of DEGs (differentially expressed genes) involved in the flavonoid biosynthesis pathway was promoted, but the expression of all or almost all DEGs associated with photosynthesis-antenna proteins and the photosynthesis pathway was inhibited in poplar leaves, suggesting that BCMV infection increased the accumulation of flavonoids but decreased photosynthesis in hosts. Gene set enrichment analysis (GSEA) illustrated that viral infection promoted the expression of genes involved in the defense response or plant-pathogen interaction. MicroRNA-seq analysis illustrated that 10 miRNA families were upregulated while 6 families were downregulated in diseased poplar leaves; moreover, miR156, the largest family with the most miRNA members and target genes, was only differentially upregulated in long-period disease (LD) poplar leaves. Integrated transcriptome and miRNA-seq analyses revealed 29 and 145 candidate miRNA−target gene pairs; however, only 17 and 76 pairs, accounting for 2.2% and 3.2% of all DEGs, were authentically negatively regulated in short-period disease (SD) and LD leaves, respectively. Interestingly, 4 miR156/SPL (squamosa promoter-binding-like protein) miRNA−target gene pairs were identified in LD leaves: the miR156 molecules were upregulated, but SPL genes were downregulated. In conclusion, BCMV infection significantly changed transcriptional and posttranscriptional gene expression in poplar leaves, inhibited photosynthesis, increased the accumulation of flavonoids, induced systematic mosaic symptoms, and decreased physiological performance in diseased poplar leaves. This study elucidated the fine-tuned regulation of poplar gene expression by BCMV; moreover, the results also suggested that miR156/SPL modules played important roles in the virus response and development of viral systematic symptoms in plant virus disease.

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“…Yield loss can reach 100% in severely BCMVinfected bean fields (Saqib et al 2010;Li et al 2014), and the virus is internationally distributed. Isolates of BCMV have been identified and characterized from different countries, including USA, India, China, Iran, South Korea, Turkey, and Australia, based on analysis of complete or partial genome sequences (Flores-Estévez et al 2000;Saqib et al 2005;Damayanti et al 2008;Cui et al 2014;Seo et al 2015;Jang et al 2018). BCMV is divided into strains based on host symptoms, serological relationships, high performance liquid chromatography peptide mapping, and genome sequence (Drijfhout et al 1978;McKern et al 1992;Mink and Silbernagel 1992;Feng et al 2014aFeng et al , 2014bLi et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Development of a recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of bean common mosaic virus

et al. 2021

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Bean common mosaic virus (BCMV) is one of the most widespread and damaging viruses of cultivated legumes in the world. In addition to serious yield reduction and germplasm decline, BCMV infection also makes legumes more vulnerable to other pathogens. Early diagnosis of the virus is particularly important in limiting its spread. Recombinase polymerase amplification (RPA) is a novel isothermic amplification technology. The whole reaction can be done outside the laboratory environment after the nucleic acid sample is obtained. In this study, we established a rapid and sensitive RPA combined with the lateral flow dipstick (LFD) assay for detection of BCMV, based on the conserved BCMV coat protein (CP) gene sequence. Specific primers and a probe were designed, which amplify ~ 150 bp CP fragments from BCMV-infected samples under a constant temperature of 37 °C for 20 min. The end-labeled amplification products were detected by high-affinity LFD within 5 min. Sensitivity of this RPA-LFD assay was 1000 times greater than that of the conventional polymerase chain reaction (PCR) assay. Furthermore, when the primers/probe were used against related potyviruses including soybean mosaic virus (SMV), bean yellow mosaic virus (BYMV), and turnip mosaic virus (TuMV), the three potyviruses were not detected, indicating that the assay was BCMV species-specific. The RPA-LFD assay was also successfully applied for the detection of seed-borne BCMV in beans. The RPA-LFD assay has great potential application in the rapid diagnosis of BCMV in the field.

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First Report of Bean common mosaic virus in Cudrania tricuspidata in Korea

Cited by 6 publications

References 0 publications

Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing

Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing

Integrated transcriptome and microRNA sequencing analyses reveal gene responses in poplar leaves infected by the novel pathogen bean common mosaic virus (BCMV)

Development of a recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of bean common mosaic virus

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