First Report of <i>Aphelenchoides besseyi</i> Infecting the Aerial Part of Cotton Plants in Brazil

Favoreto, L.; Faleiro, V. O.; Freitas, M. A.; Brauwers, L. R.; Galbieri, Rafael; Homiak, Juliana Aparecida; Lopes-Caitar, Valéria Stefania; Marcelino-Guimarães, Francismar Corrêa; Meyer, M. C.

doi:10.1094/pdis-02-18-0334-pdn

Cited by 15 publications

(15 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an example, the evolution of plant parasitism in nematodes is reported for Aphelenchoides spp., which feed and reproduce on plant leaf mesophyll and on buds (as a migratory endoparasite) but can also feed on fungi [45]. Recently, this group was reported as the causal agent of a new soybean disease in Brazil, the soybean green stem and foliar retention syndrome [46], and also causing similar symptoms in cotton plants [47], which shows the great plasticity of Aphelenchoides spp. in adaptation to hosts imposed in the cropping systems.…”

Section: Discussionmentioning

confidence: 99%

Two novel potential pathogens for soybean

2019

View full text Add to dashboard Cite

Nematode diseases have a worldwide importance for several economic agricultural crops, such as soybean. Frequently, new or secondary pathogens arise as emergent diseases due to the intensified use of agricultural lands, causing extensive yield losses. Helicotylenchus dihystera (Hd) and Scutellonema brachyurus (Sb) appear as potential pathogens for soybean in Brazil, since their spread and population densities have been increased on soybean growing areas. Aiming to evaluate the quantitative distribution of Hd and Sb in soybean fields in South Brazil, a survey was conducted during the growing seasons of 2014–2015 and 2015–2016 in which 1,088 soil samples and 1,043 root samples were analyzed. Besides, two greenhouse experiments were conducted to evaluate the pathogenicity of both nematodes to soybean plants, in comparison with P . brachyurus (Pb), a known pathogen of the crop. The survey demonstrated that Pb, Hd and Sb are widely distributed in the States of Paraná, Santa Catarina and Mato Grosso do Sul. Besides, we proved that Hd and Sb multiply and cause root lesions in soybean cv. Potência, since they were found inside roots, and can be considered as potential pathogens for soybean plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Two novel potential pathogens for soybean

2019

View full text Add to dashboard Cite

show abstract

“…The nematodal population in the roots was assessed as described previously, and 170–210 nematodes were present following the inoculation period. The decrease in the nematode population in the roots was expected, as they will migrate to other parts of the plant . Untargeted MS revealed molecular differences between healthy soybean roots and those inoculated with A. besseyi .…”

Section: Resultsmentioning

confidence: 99%

“…The nematodes used for multiplication were axenized in 0.1% ampicillin solution for 10 min. Then, a total of 20 specimens (15 females and 5 males) were transferred to each plate with a 6‐day‐old colony of Fusarium sp. These plates were kept in the BOD chamber at 25°C (± 1°C) for 25 days when the amounts of nematodes were sufficient to be inoculated onto hydroponic soybean plants.…”

Section: Methodsmentioning

confidence: 99%

Metabolomics of soybean green stem and foliar retention (GSFR) disease using mass spectrometry and molecular networking

Zanzarin

Hernandes

Leme³

et al. 2020

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

Rationale The nematode Aphelenchoides besseyi is the causal agent of green stem and foliar retention, a soybean disease recently described in Brazil. This condition can reduce soybean yield by up to 100%. However, little is known about chemical interactions between the plant and pathogen. Therefore, this work aimed to investigate metabolites from healthy soybean roots and from soybean roots that were inoculated with A. besseyi. Methods A. besseyi were multiplied in vitro with Fusarium sp. colonies in Petri dishes for 25 days, and were axenically inoculated into hydroponics healthy soybean plants. The metabolites were extracted from the roots of healthy and A. besseyi‐infected plants 16 days post‐inoculation. These extracts were analyzed using an untargeted metabolomic method with an ultra‐high‐performance liquid chromatography/electrospray ionization /tandem mass spectrometry (UHPLC/ESI‐MS/MS) and molecular networking approach. Results Roots from infected plants showed morphological alterations such as shrinkage, darkening, and arching. Similarly, they also showed an increased presence of flavonoids, compared with healthy roots. Compounds such as neobavaisoflavone, glycitin, genistin, and genistein were putatively identified and had greater intensity in inoculated roots. These compounds are linked to the defensive mechanisms in plants against nematodes. Moreover, coumaric acid, also exclusively putatively identified in inoculated roots, shows activity related to inhibition of root growth. Conclusions Liquid chromatography, mass spectrometry, and molecular networking approaches proved to be a powerful tool for the metabolomic study of GSFR. This study showed metabolomics differences of protective substances in the roots, evidencing a quick response of the plant to the attack of A. besseyi.

show abstract

“…The cost of water, Tuotulong, Ethephon, Sujie and Kesheng are 0.0028 CNY/L, 0.15, 0.06, 0.26 and 0.14 CNY/mL respectively. Working efficiency is calculated by spraying swath width and flight speed of aircraft by the following equation: E=S×W (8) where, E is efficiency, m 2 /s; S is flight speed, m/s; and W is swath width, m.…”

Section: Data Processing and Sprays Assessing Methodsmentioning

confidence: 99%

“…Martin et al [5] researched aerial electrostatic spray deposition and canopy penetration in cotton. In addition, aerial pesticide spraying has been applied in Swaziland [6] , Australia [7] , Brazil [8] and other countries. Xin et al [9] studied the effects of dosage and spraying volume on cotton defoliants efficacy using Unmanned Aerial Vehicle (UAV) in China.…”

Section: Introduction mentioning

confidence: 99%

Optimization of variables for maximizing efficacy and efficiency in aerial spray application to cotton using unmanned aerial systems

Liao

Zhang

Luo

et al. 2019

International Journal of Agricultural and Biological Engineering

View full text Add to dashboard Cite

Aerial spraying can support efficient defoliation without crop contact. With the recent introduction to unmanned aerial system (UAS) for aerial spraying in China, there is a need to determine the optimum application variables to achieve high efficacy and efficiency with low costs. The present research involved field studies across two annual cotton production seasons in North Xinjiang, China. Four factors, including volume rate (A), tank mix including spray adjuvants (B), flight altitude (C), flight speed (D) and three levels of L 9 (3 4) orthogonal arrays were carried out to optimize the application parameters for three types of UASs. These included different numbers of rotors as follows: four-rotors, six-rotors and eight-rotors. Spray coverage, distribution uniformity (coefficient of variation (CV) of droplet coverage), rates of cotton defoliation and boll opening, application efficiency and cost were measured and assessed. Results showed that: (1) the rates of defoliation and boll opening by aerial cotton defoliant application could meet the requirement of cotton mechanized harvesting; (2) the optimal scenario for the three UASs was A 3 B 2 C 1 D 3 , Volume rate (A 3): 48 L/hm 2 ; Tank mix and concentration (B 2): (Tuotulong 225 + Sujie 750 + Ethephon 2250) mL/hm 2 , Flight altitude (C 1): 1.5 m, and Flight speeds (D 3) for unmanned helicopters with four-rotors, six-rotors and eight-rotors were 3.12 m/s, 2.51 m/s and 3.76 m/s, respectively. These results can provide guidance for cotton defoliant aerial spraying in China using UAS.

show abstract

First Report of Aphelenchoides besseyi Infecting the Aerial Part of Cotton Plants in Brazil

Cited by 15 publications

References 3 publications

Two novel potential pathogens for soybean

Two novel potential pathogens for soybean

Metabolomics of soybean green stem and foliar retention (GSFR) disease using mass spectrometry and molecular networking

Optimization of variables for maximizing efficacy and efficiency in aerial spray application to cotton using unmanned aerial systems

Contact Info

Product

Resources

About