First report of <i>Alternaria mali</i> causing necrotic leaf spot of apples in Turkey

Özgönen, Hülya; Karaca, Gürsel

doi:10.1111/j.1365-3059.2006.01372.x

Cited by 14 publications

(8 citation statements)

References 2 publications

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“…Penetration of host plants through wounds has been shown to be a significant component of infection process for a number of Alternaria species (Rotem, 1994). Ozgonen and Karaca (2006) and Soleimani and Esmailzadeh (2007) also confirmed the pathogenicity of A. mali isolates by inoculation with conidial suspension on detached wounded apple leaves.…”

Section: Resultsmentioning

confidence: 84%

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

T¹,

Muzafer²,

Gh³

et al. 2013

Afr. J. Biotechnol.

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Section: Resultsmentioning

confidence: 84%

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

T¹,

Muzafer²,

Gh³

et al. 2013

Afr. J. Biotechnol.

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“…Leaf blotch mostly affects high-value and very popular cultivars, such as the ‘Fuji’, ‘Royal Gala’, ‘Red Delicious’, and ‘Pink Lady’ cultivars 39 . A. mali , referred to as the apple Alternaria leaf blotch pathotype, has been reported in Japan 40 , China 41 , Korea 42 , the United States 43 , Russia 44 , Yugoslavia 45 , Iran 46 , Turkey 47 , and Brazil 48 . Few studies have investigated Alternaria leaf blotch control and resistance genes.…”

Section: Discussionmentioning

confidence: 99%

Two pathogenesis-related proteins interact with leucine-rich repeat proteins to promote Alternaria leaf spot resistance in apple

Zhang

Wei

et al. 2021

Hortic Res

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Alternaria leaf spot in apple (Malus x domestica), caused by the fungal pathogen Alternaria alternata f. sp. mali (also called A. mali), is a devastating disease resulting in substantial economic losses. We previously established that the resistance (R) protein MdRNL2, containing a coiled-coil, nucleotide-binding, and leucine-rich repeat (CCR-NB-LRR) domain, interacts with another CCR-NB-LRR protein, MdRNL6, to form a MdRNL2–MdRNL6 complex that confers resistance to A. mali. Here, to investigate the function of the MdRNL2–MdRNL6 complex, we identified two novel pathogenesis-related (PR) proteins, MdPR10-1 and MdPR10-2, that interact with MdRNL2. Yeast two-hybrid (Y2H) assays and bimolecular fluorescence complementation (BiFC) assays confirmed that MdPR10-1 and MdPR10-2 interact with MdRNL2 and MdRNL6 at the leucine-rich repeat domain. Transient expression assays demonstrated that accumulation of MdPR10-1 and MdPR10-2 enhanced the resistance of apple to four strains of A. mali that we tested: ALT1, GBYB2, BXSB5, and BXSB7. In vitro antifungal activity assays demonstrated that both the proteins contribute to Alternaria leaf spot resistance by inhibiting fungal growth. Our data provide evidence for a novel regulatory mechanism in which MdRNL2 and MdRNL6 interact with MdPR10-1 and MdPR10-2 to inhibit fungal growth, thereby contributing to Alternaria leaf spot resistance in apple. The identification of these two novel PR proteins will facilitate breeding for fungal disease resistance in apple.

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“…Alternaria species to confirm its pathogenicity (Rotem, 1994;Kumar, 2004;Quayyum et al, 2005;Ozgonen and Karaca, 2006;Soleimani and Esmailzadeh, 2007) and ALT12WT2, ALT14KP3, ALT12CR2 and ALT14KK5 were found to be highly virulent. The variations in amino acids sequences may be attributed to the diverse geographical distribution of these isolates.…”

Section: Detach Leaf Assay Technique Is More Reliable Formentioning

confidence: 99%

“…These tools were also employed for the confirmation of A. mali in Yugoslavia, Quebec, Manitoba, North Carolina, Indiana, Florida, Iran and Turkey (Jones and Aldwinckle, 1990;Bulajic et al, 1996;Ozgonen and Karaca, 2006;Soleimani and Esmailzadeh, 2007).…”

Section: Detach Leaf Assay Technique Is More Reliable Formentioning

confidence: 99%

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with necrotic leaf spot of loquat

2016

Int. J. Biosci.

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In Pakistan, loquat (Eriobotrya japonica) in an emerging fruit and provides well economic return to growers.Percentage disease incidence of necrotic leaf spot (Alternaria mali) ranged from 25 to 46.16% in Taxila, Wahcant, Khanpur, Kalar Kahar, Choa Saiden Shan, Tret, Chatar and Murree. 48 isolates were characterized for cultural, morphological and pathogenic variations. Light to dark olivacious colonies were appraised, cottony to velvety with regular to irregular margins and variations in size and shape of conidia were observed between isolates. Length of conidiophores was ranging from 24.6 to 68.5 µm and nine was the highest septation in isolate ALT7WT1. Nucleotide sequences of inter transcribed spacer region (ITS1, 5.8S, ITS2) from 4 highly virulent isolates were submitted in the database of NCBI under accession numbersKR232489, KR232490, KT154010 and KT154011 respectively. Evolutionary history was computed with available sequences of A. mali, A. lternata, A. zinnae and A. tenuissima which were reported from Pakistan, USA, Sweden, Newzeland and India. Evolutionary tree showed4 different sub-trees and submitted sequences were observed in A. mali sub-tree. Amino acid sequences were exhibiting 100 to 99% genetic homology with A. mali isolate (FCBP1343) reported form Pakistan. Amino acid sequences were compared with FCBP1343 and differences at position number 18 (valine was replaced with glutamate), 57 (glutamine with leucine), 84 (alanine with glycine), 163 (glutamine instead of histidine) and 171 (serine was replaced with phenylalanine) were observed. Cultural, morphological, pathogenic and molecular characterization of A. mali associated with necrotic leaf spot of loquat was recorded first time in Pakistan.

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First report of Alternaria mali causing necrotic leaf spot of apples in Turkey

Cited by 14 publications

References 2 publications

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

Two pathogenesis-related proteins interact with leucine-rich repeat proteins to promote Alternaria leaf spot resistance in apple

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with necrotic leaf spot of loquat

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