First Report of Human Urinary Tract Infection Caused by Lactococcus petauri

Colussi, Silvia; Pastorino, Paolo; Prearo, Marino; Sciuto, Simona; Bondavalli, Fabio; Acutis, Pier Luigi; Bozzetta, Elena; Amisano, Francesco; Salerno, Angelo

doi:10.3390/microorganisms11102583

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…• The identification of L. petauri in a new host species expands our knowledge of its host tropism; • L. petauri could have implications for public health, either directly or indirectly. While L. petauri may not pose an immediate threat to human health, understanding its distribution and hosts can be relevant for managing and monitoring potential risks, especially if its zoonotic potential is confirmed [10].…”

Section: Discussionmentioning

confidence: 99%

“…The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/fishes9040117/s1, Table S1: A summary of the interpretive categories and breakpoints (mm) (streptococci species were used). Interpretative criteria outlined by the Clinical and Laboratory Standards Institute (CLSI) and the studies on L. petauri conducted by Cataõ Egger and colleagues in 2022 [10]. Author Contributions: Conceptualization, P.P., S.C., and M.P.…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…In 2019, it was isolated in rainbow trout (Oncorhynchus mykiss) affected by lactococcosis in Greece [3]; it was later described in the United States [4,5], Turkey [6], Spain [7,8], and in Nile tilapia (Oreochromis niloticus) in Brazil [9], but it was never observed in reared and wild fishes in Italy. Nevertheless, recently a case of human urinary tract infection caused by L. petauri was reported in Italy [10].…”

Section: Introductionmentioning

confidence: 99%

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First Report of Lactococcus petauri in the Pumpkinseed (Lepomis gibbosus) from Candia Lake (Northwestern Italy)

Bondavalli,

Colussi,

Pastorino

et al. 2024

Fishes

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Lactococcus petauri has emerged as a pathogen affecting farmed fish. In this case report, L. petauri was isolated from Lepomis gibbosus captured in Lake Candia, located in Piedmont, Northwestern Italy. Initially identified as L. garvieae using conventional methods like RAPID 32 ID STREP and MALDI-TOF, molecular analysis based on sequencing of the 16S-23S rRNA internal transcribed spacer region (ITS) confirmed it as L. petauri. The study also assessed the isolated strain’s pathogenicity by examining hemolysin and capsule cluster genes, revealing only hemolysin 2 amplicon; notably, the fish exhibited no lesions or pathological alterations. The L. petauri strain from this study displayed resistance to several antibiotics, being sensitive only to ampicillin, amoxicillin, florfenicol, and G-penicillin. This research provides new insights into host range of L. petauri and raises awareness of potential public health implications, particularly concerning zoonotic potential.

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Section: Discussionmentioning

confidence: 99%

Section: Supplementary Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First Report of Lactococcus petauri in the Pumpkinseed (Lepomis gibbosus) from Candia Lake (Northwestern Italy)

Bondavalli,

Colussi,

Pastorino

et al. 2024

Fishes

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Changes in blood serum parameters in farmed rainbow trout (Oncorhynchus mykiss) during a piscine lactococcosis outbreak

Esposito,

Bergagna,

Colussi

et al. 2024

Journal of Fish Diseases

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The aquaculture sector plays a vital role in global food security, yet it grapples with significant challenges posed by infectious diseases. Piscine lactococcosis is one of the significant threats in rainbow trout aquaculture due to its potential to cause severe economic losses through mortalities, reduced growth rates, and increased susceptibility to other pathogens. It poses challenges in disease management strategies, impacting the sustainability and profitability of rainbow trout farming. The current study focuses on the variations in serum blood parameters of farmed rainbow trout Oncorhynchus mykiss during a lactococcosis outbreak caused by Lactococcus garvieae. Blood samples were collected for biochemical analysis, fish were examined for parasites and bacteria, and DNA from bacterial colonies was PCR‐amplified and sequenced for identification. Overall, 13 biochemical parameters, including proteins, enzymes, lipids, chemicals, and minerals, were measured in serum blood samples from both diseased and healthy fish. The results indicate significant alterations in the levels of these parameters during the outbreak, highlighting the impact of infections on the blood profile of farmed rainbow trout. Urea levels were significantly higher in diseased fish compared to controls, and creatinine, phosphorus, and magnesium also showed similar trends. Alanine aminotransferase and total protein levels were higher in control fish. Chloride levels differed significantly between groups. Iron levels were higher in controls and lower in diseased fish. No significant differences were found in other parameters. This study reveals significant changes in serum blood parameters of rainbow trout during a lactococcosis outbreak caused by L. garvieae. These changes highlight the potential of these parameters as tools for monitoring health status, stress, and aquaculture management. Continuous monitoring can provide valuable insights into disease severity and overall fish health, aiding in the development of improved management practices. The presented data contribute to understanding the pathophysiology of piscine lactococcosis and developing effective mitigation strategies for farmed rainbow trout.

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Genomic investigation of Lactococcus formosensis , Lactococcus garvieae , and Lactococcus petauri reveals differences in species distribution by human and animal sources

Chan,

Cao,

Jiang

et al. 2024

Microbiol Spectr

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Lactococcus garvieae is a fish pathogen that can cause diseases in humans and cows. Two genetically related species, Lactococcus formosensis and Lactococcus petauri , may be misidentified as L. garvieae . It is unclear if these species differ in host specificity and virulence genes. This study analyzed the genomes of 120 L . petauri , 53 L . formosensis , and 39 L . garvieae isolates from various sources. The genetic diversity and virulence gene content of these isolates were compared. The results showed that 77 isolates previously reported as L. garvieae were actually L. formosensis or L. petauri . The distribution of the three species varied across different collection sources, with L. petauri being predominant in human infections, human fecal sources, and rainbow trout, while L. formosensis was more common in bovine isolates. The genetic diversity of isolates within each species was high and similar. Using a genomic clustering method, L. petauri , L. formosensis , and L. garvieae were divided into 45, 22, and 13 clusters, respectively. Most rainbow trout and human isolates of L. petauri belonged to different clusters, while L. formosensis isolates from bovine and human sources were also segregated into separate clusters. In L. garvieae , most human isolates were grouped into three clusters that also included isolates from food or other sources. Non-metric multidimensional scaling ordination revealed the differential association of 15 virulence genes, including 14 adherence genes and a bile salt hydrolase gene, with bacterial species and certain collection sources. In conclusion, this work provides evidence of host specificity among the three species. IMPORTANCE Lactococcus formosensis and Lactococcus petauri are two newly discovered bacteria, which are closely related to Lactococcus garvieae, a pathogen that affects farmed rainbow trout, as well as causes cow mastitis and human infections. It is unclear whether the three bacteria differ in their host preference and the presence of genes that contribute to the development of disease. This study shows that L. formosensis and L. petauri were commonly misidentified as L. garvieae . The three bacteria showed different distribution patterns across various sources. L. petauri was predominantly found in human infections and rainbow trout, while L. formosensis was more commonly detected in cow mastitis. Fifteen genes displayed a differential distribution among the three bacteria from certain sources, indicating a genetic basis for the observed host preference. This work indicates the importance of differentiating the three bacteria in diagnostic laboratories for surveillance and outbreak investigation purposes.

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First Report of Human Urinary Tract Infection Caused by Lactococcus petauri

Cited by 3 publications

References 23 publications

First Report of Lactococcus petauri in the Pumpkinseed (Lepomis gibbosus) from Candia Lake (Northwestern Italy)

First Report of Lactococcus petauri in the Pumpkinseed (Lepomis gibbosus) from Candia Lake (Northwestern Italy)

Changes in blood serum parameters in farmed rainbow trout (Oncorhynchus mykiss) during a piscine lactococcosis outbreak

Genomic investigation of Lactococcus formosensis , Lactococcus garvieae , and Lactococcus petauri reveals differences in species distribution by human and animal sources

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