First report of geosmin and 2-methylisoborneol (2-MIB) in Dolichospermum and Oscillatoria from Vietnam

Pham, Thanh-Luu; Bui, Ha Manh; Driscoll, Mark; Shimizu, Kazuya; Utsumi, Motoo

doi:10.1007/s10201-020-00630-2

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…Sphaerospermopsis, Cylindrospermopsis and Nodularia were investigated genera in this study which were already reported as GEO or MIB producer in previous studies (Popin et al 2016, Watson et al 2016, Zhang et al 2017, Pham et al 2020. Also, Microcystis strains were investigated with the knowledge of other coccoid cyanobacteria such as Synechococcus C.Nägeli or Coelosphaerium Nägeli as a producer (Kutovaya & Watson 2014, Godo et al 2017).…”

Section: Dolichospermummentioning

confidence: 99%

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The First Report of Geosmin and 2-Methylisoborneol Producer Cyanobacteria From Turkish Freshwaters

Tunç

Akçaalan

Köker

et al. 2021

Trakya University Journal of Natural Sciences

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Water users consider the safety of water according to its aesthetic properties, primarily taste and odour. Geosmin (GEO) and 2-methylisoborneol (MIB) are the most common taste and odour compounds in freshwaters which cause an earthy and musty odour in water. Since human nose can detect these compounds in concentrations as low as 10 ng/L, it is essential to monitor drinking waters before consumer complaints and to produce a timely solution. Therefore, it is necessary to identify GEO and MIB producers to manage the problem at its source. Cyanobacteria are one of the main producers of these compounds in freshwater ecosystems. In this study, we analyzed 13 samples (9 cyanobacteria cultures from Bafa Lake, Elmalı Dam Lake, İznik Lake, Küçükçekmece Lake, Manyas Lake and Taşkısığı Lake, and 4 environmental water samples from Erfelek and Günpınar Waterfalls and Ömerli Dam Lake) for GEO and MIB production by HS-SPME (Head space-solid phase microextraction) coupled with GC-MS (gas chromatography-mass spectrometry). The presence of Cyanobacteriaspecific GEO and MIB synthase genes were also analyzed by PCR (Polymerase Chain Reaction). Taste and odour production was confirmed in 2 samples by GC-MS while 4 samples yielded positive results by PCR. All positive samples were environmental samples (3 samples from waterfalls from Günpınar and Erfelek Waterfalls, 1 sample from Ömerli Dam Lake -a drinking water reservoir) which were dominated by Nostoc Vaucher ex Bornet & Flahault, Phormidium Kützing ex Gomont and Pseudanabaena Lauterborn. This is the first report of GEO and MIB producing cyanobacteria in Turkish freshwaters by combining microscopy, analytical and molecular techniques.Özet: Su kullanıcıları, suyun güvenli olup olmadıklarına öncelikle onun tat ve kokusu gibi estetik özelliklerine bakarak karar vermektedir. Geosmin (GEO) ve 2-methylisoborneol (MIB), tatlısularda en yaygın olarak görülen tat ve koku bileşikleridir ve suyun toprak ve küf kokmasına neden olurlar. İnsanlar <10 ng/L gibi düşük konsantrasyonlarda dahi bu kokulara hassas olmalarından dolayı bu bileşiklerin içme sularında tüketici şikayetleri oluşmadan önce izlenmesi ve sorunun çözülmesi oldukça önemlidir. Bu sebeple, problemin kaynağında çözümlenebilmesi için GEO ve MIB üreticilerinin tespit edilmesi gereklidir. Tatlısu ekosistemlerinde bu bileşiklerin başlıca üreticilerinden biri siyanobakterilerdir (Cyanobacteria). Bu çalışmada 13 örnek (9 siyanobakteri kültürü, Bafa Gölü, Elmalı Baraj Gölü, İznik Gölü, Küçükçekmece Gölü, Manyas Gölü, Taşkısığı Gölü'nden ve 4 çevresel su örneği, Günpınar, Erfelek şelaleleri ve Ömerli Baraj Gölü'nden) GEO ve MIB üretiminin tespiti için HS-SPME (Tepe Boşluğu-Katı Faz Mikro Ekstraksiyon) GC-MS (Gaz Kromatografi-Kütle Spektrometresi) yöntemi kullanılarak analiz edilmiştir. Ayrıca siyanobakterilere özgü GEO ve MIB sentaz genlerinin varlığının tespiti için PZR (Polimeraz Zincir Reaksiyonu) yöntemi kullanılmıştır. İki örnekte GC-MS ile tat ve koku üretimi tespit edilmiş ve 4 örnekte de PZR ile pozitif sonuç alınmıştır. Pozitif son...

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Section: Dolichospermummentioning

confidence: 99%

“…Geosmin (GEO) and 2-methylisoborneol (MIB) are the most common biogenic taste and odour compounds in freshwaters and considered as indicators of water quality by consumers (Webber et al 2015, Pham et al 2020). Therefore, some countries set a guideline value as 10 ng/L in their drinking waters (Wakayama 2003, NHMRC 2011.…”

Section: Introductionmentioning

confidence: 99%

The First Report of Geosmin and 2-Methylisoborneol Producer Cyanobacteria From Turkish Freshwaters

Tunç

Akçaalan

Köker

et al. 2021

Trakya University Journal of Natural Sciences

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“…When HCBs appear in a reservoir, appearance and musty-odor problems can make humans uncomfortable. In particular, the musty-odor (earth odor, ink odor) that is continuously generated in water systems such as reservoirs and lakes around the world has become an extremely serious problem in drinking water source ponds [8][9][10][11][12] . If a large amount of the musty odor is created, it requires activated carbon treatment, which causes economic loss.…”

Section: Introductionmentioning

confidence: 99%

Effect of Rainfall and pH on Musty Odor Produced in the Sanbe Reservoir

Kim

Hayashi

Masuki

et al. 2021

Preprint

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Harmful cyanobacterial blooms (HCBs) are continuously formed in water systems such as reservoirs and lakes around the world. In particular, geosmin and 2-methylisoborneol (2-MIB), which are made by cyanobacteria, have caused serious musty-odor problems in the drinking water of the Sanbe reservoir in Japan. To investigate the cause of this musty odor, we conducted field observations for five years (2015–2019). As a result, it was determined that the geosmin was caused by the cyanobacteria as Dolichospermum crassum and Dolichospermum planctonicum, and 2-MIB was caused by the cyanobacteria as Pseudanabaena sp and Aphanizomenon cf. flos-aquae. It was also determined that the occurrence of geosmin and 2-MIB are influenced by changes in water temperature and pH due to the inflow of rainfall. Especially, the geosmin and 2-MIB tended to occur in low rainfall conditions. When there is low rainfall, the reservoir changes to an alkaline state due to the consumption of CO2 by the photosynthesis of phytoplankton. In an alkaline reservoir, CO2 exists in the form of bicarbonate (HCO3-); thus, under these condition cyanobacteria will increase because it uses both CO2 and bicarbonate (HCO3-) for photosynthesis. Our study suggests that for solving the musty-odor problem in the reservoir, it is important to control the reservoir’s pH. Moreover, more focus should be place in managing the water quality problem of the Sanbe Reservoir, especially, during low rainfall years. The results of this study will help in addressing odor related water quality problem in other reservoirs.

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“…When HCBs appear in a reservoir, appearance and musty odor problems can make humans uncomfortable. The musty odor (earth odor and ink odor) that is continuously generated in water systems such as reservoirs and lakes around the world has become an extremely serious problem in drinking water source ponds [8][9][10][11][12][13][14]. If a large amount of musty odor is produced, it requires activated carbon treatment, which causes economic loss.…”

Section: Introductionmentioning

confidence: 99%

Effect of Rainfall and pH on Musty Odor Produced in the Sanbe Reservoir

Kim

Hayashi

Masuki

et al. 2021

Water

View full text Add to dashboard Cite

Harmful cyanobacterial blooms are continuously formed in water systems such as reservoirs and lakes around the world. Geosmin and 2-methylisoborneol (2-MIB) produced by some species of cyanobacteria have caused odor problems in the drinking water of the Sanbe Reservoir in Japan. Field observations were conducted for four years (2015–2019) to investigate the cause of this musty odor. It was found that geosmin was produced by Dolichospermum crassum and Dolichospermum planctonicum (cyanobacteria), and 2-MIB was due to Pseudanabaena sp. and Aphanizomenon cf. flos-aquae (cyanobacteria). Changes in water temperature and pH caused by rainfall were correlated with changes in the concentration of geosmin and 2-MIB. In particular, geosmin and 2-MIB tended to occur under low rainfall conditions. When there was low rainfall, the reservoir changed to an alkaline state because the phytoplankton consumed CO2 for photosynthesis. In an alkaline reservoir, dissolved inorganic carbon mainly existed in the form of bicarbonate (HCO3−). Thus, the results suggest that under such conditions in reservoirs, cyanobacteria grew easily because they could use both CO2 and HCO3− for photosynthesis. Specifically, our study suggests that in order for the musty odor problem in the reservoir to be solved, it is important that the pH of the reservoir be controlled.

show abstract

First report of geosmin and 2-methylisoborneol (2-MIB) in Dolichospermum and Oscillatoria from Vietnam

Cited by 10 publications

References 35 publications

The First Report of Geosmin and 2-Methylisoborneol Producer Cyanobacteria From Turkish Freshwaters

The First Report of Geosmin and 2-Methylisoborneol Producer Cyanobacteria From Turkish Freshwaters

Effect of Rainfall and pH on Musty Odor Produced in the Sanbe Reservoir

Effect of Rainfall and pH on Musty Odor Produced in the Sanbe Reservoir

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