First report of Fusarium solani species complex as a causal agent of Erythrina variegata decline and death after gall formation by Quadrastichus erythrinae on Okinawa Island, Japan

Kuroda, Kazuyuki; Chuma, Izumi; Kihara, Takeo; Murakami, Tsubasa; Takashina, Kuya; Hiraoka, Daiki; Kameyama, Norikazu

doi:10.1007/s10327-017-0738-3

Cited by 4 publications

(3 citation statements)

References 30 publications

(44 reference statements)

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“…FSSC was reportedly associated with other Scolytidae, including Euwallacea fornicatus (Eichhoff) [53], Xyleborus ferrugineus (Fabricius) [52], and Xylosandrus compactus (Eichhoff) [55], and had a wide plant host range, including avocado (Persea americana Miller) [53], coffee (Coffea arabica L.) [63], Japanese cheesewood (Pittosporum tobira (Thunb. ex Murray)) [64], and the Indian coral tree (Erythrina variegata L.) [65,66]. Euwallacea interjectus shows an exclusive relationship with Fusarium floridanum Aoki, Smith, Kasson, Freeman, Geiser et O'Donnell (Hypocreales: Nectriaceae) on box elder in Florida, USA [25] and F. kuroshium on fig trees in Hiroshima, Japan [34].…”

Section: Discussionmentioning

confidence: 99%

Fungal Flora in Adult Females of the Rearing Population of Ambrosia Beetle Euwallacea interjectus (Blandford) (Coleoptera: Curculionidae: Scolytinae): Does It Differ from the Wild Population?

2022

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Ambrosia beetles bore into host trees, and live with fungi symbiotically that serve as a food source. However, it is challenging to directly observe these beetles in the wild. In this study, Euwallacea interjectus Blandford (Coleoptera: Curculionidae: Scolytinae), a pest of fig trees in Japan, were reared under artificial conditions to emulate the behavior of Ambrosia beetle. Fungi were isolated from the adult females of E. interjectus to identify the species associated with secondary symbiosis. In total, nine filamentous fungi and one yeast were identified using morphological characteristics and DNA sequence data. Neocosmospora metavorans (Hypocreales: Nectriaceae), Fusarium sp. (Hypocreales: Nectriaceae), that is undescribed, and Meyerozyma guilliermondii (Saccharomycetes: Saccharomycetales) (yeast) were isolated more frequently from the head (including from mycangia, the fungus-carrying organ) than from the thorax and abdomen of adult beetles. Neocosmospora metavorans was the dominant species isolated from 12 out of 16 heads at 200 to 3300 CFUs/head, compared to the primary mycangia fungus from wild beetles, i.e., Fusarium kuroshium (Hypocreales: Nectriaceae). Temperature had a marked effect on fungal growth in the three symbiont species. Our results represent a major paradigm shift in understanding beetle–fungal interactions, as they show specific symbiont switching can occur in different nesting places.

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Section: Discussionmentioning

confidence: 99%

Fungal Flora in Adult Females of the Rearing Population of Ambrosia Beetle Euwallacea interjectus (Blandford) (Coleoptera: Curculionidae: Scolytinae): Does It Differ from the Wild Population?

2022

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“…Neocosmospora metavorans has a wide range of plant hosts including avocado [ 24 ] and robusta coffee ( Coffea canephora Pierre ex Froehner) [ 25 ]. Moreover, FSSC was primarily detected in discolored xylem and necrotic phloem from declining and defoliated Indian coral tree ( Erythrina variegata L.) in Japan [ 26 , 27 ]. However, there have been no reports on the role of AFC in FWD.…”

Section: Introductionmentioning

confidence: 99%

The Role of Mycangial Fungi Associated with Ambrosia Beetles (Euwallacea interjectus) in Fig Wilt Disease: Dual Inoculation of Fusarium kuroshium and Ceratocystis ficicola Can Bring Fig Saplings to Early Symptom Development

Jiang

Morita²,

Jikumaru³

et al. 2022

Microorganisms

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The ambrosia beetle, Euwallacea interjectus, is a wood-boring pest and a vector of Ceratocystis ficicola, a pathogenic fungus causing fig (Ficus carica) wilt disease (FWD) in Japan. The ambrosia fungi, Fusarium kuroshium and Neocosmospora metavorans, have been frequently isolated from heads (including mycangia) of wild and reared adult female E. interjectus, respectively. However, the exact mechanisms driving FWD as well as the interactions between F. kuroshium and C. ficicola in fig orchard remain unclear. To verify the role of the mycangial fungi in the FWD progression, fig saplings were subjected to inoculation treatments (T1, F. kuroshium; T2, N. metavorans, reference positive control; T3, C. ficicola; T4, F. kuroshium + C. ficicola, realistic on-site combination). T3 and T4 saplings began wilting approximately 12 days after inoculation, leading to eventual death. Median duration from inoculation to death of the T4 saplings was approximately four days significantly faster than that of the T3 saplings. Xylem sap-conduction test indicated that dysfunction and necrosis area were considerably wider in the T4 saplings than in T3 saplings. These results demonstrate that the synergistic action of F. kuroshium and C. ficicola contributed to accelerated wilting in the saplings. Based on these discoveries, we proposed a model for system changes in the symbiosis between E. interjectus and its associated fungi in FWD in Japan.

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“…Recently, some species in the FSSC, including the AFC species, were reassigned to the genus Neocosmospora based on gene sequences and morphology (Sandoval-Denis and Crous, 2018;Sandoval-Denis et al, 2019;Veloso et al, 2021). Previous studies have identified the phenotypic characteristics that can be used to distinguish the AFC species (Aoki et al, 2018;Lynn et al, 2021) and elucidate their host range and pathogenic potential (Eskalen et al, 2013;Kuroda et al, 2017). This provided a basis for the development, management, monitoring, and eradication strategies.…”

Section: Introductionmentioning

confidence: 99%

Novel Symbiotic Association Between Euwallacea Ambrosia Beetle and Fusarium Fungus on Fig Trees in Japan

2021

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Ficus carica plantations in Japan were first reported to be infested by an ambrosia beetle species, identified as Euwallacea interjectus, in 1996. The purpose of this study was to determine the symbiotic fungi of female adults of E. interjectus emerging from F. carica trees infected with fig wilt disease (FWD). Dispersal adults (51 females) of E. interjectus, which were collected from logs of an infested fig tree in Hiroshima Prefecture, Western Japan, were separated into three respective body parts (head, thorax, and abdomen) and used for fungal isolation. Isolated fungi were identified based on the morphological characteristics and DNA sequence data. Over 13 species of associated fungi were detected, of which a specific fungus, Fusarium kuroshium, was dominant in female head (including oral mycangia). The plant-pathogenic fungus of FWD, Ceratocystis ficicola, was not observed within any body parts of E. interjectus. We further discussed the relationship among E. interjectus and its associated fungi in fig tree.

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First report of Fusarium solani species complex as a causal agent of Erythrina variegata decline and death after gall formation by Quadrastichus erythrinae on Okinawa Island, Japan

Cited by 4 publications

References 30 publications

Fungal Flora in Adult Females of the Rearing Population of Ambrosia Beetle Euwallacea interjectus (Blandford) (Coleoptera: Curculionidae: Scolytinae): Does It Differ from the Wild Population?

Fungal Flora in Adult Females of the Rearing Population of Ambrosia Beetle Euwallacea interjectus (Blandford) (Coleoptera: Curculionidae: Scolytinae): Does It Differ from the Wild Population?

The Role of Mycangial Fungi Associated with Ambrosia Beetles (Euwallacea interjectus) in Fig Wilt Disease: Dual Inoculation of Fusarium kuroshium and Ceratocystis ficicola Can Bring Fig Saplings to Early Symptom Development

Novel Symbiotic Association Between Euwallacea Ambrosia Beetle and Fusarium Fungus on Fig Trees in Japan

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