First report of entomopathogenic nematode <i>Steinernema feltiae</i> (Rhabditida: Steinernematidae) from Croatia

Majić, Ivana; Sarajlić, Ankica; Lakatos, T.; Tóth, Tímea; Raspudić, Emilija; Zebec, Vedrana Živković; Šarić, Gabriella Kanižai; Kovačić, Marin; Laznik, Žiga

doi:10.2478/helm-2018-0024

Cited by 9 publications

(7 citation statements)

References 26 publications

(30 reference statements)

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“…This result was similar to that reported by Brodie [ 31 ] from Fiji Islands (7.3%), Valadas [ 32 ] from Portugal (6.7%), and Hatting [ 33 ] from South Africa (5%). However, this rate was higher than those reported by Caoili [ 34 ] from the Philippines (2.5%), Majić [ 35 ] from Croatia (2.0%), and Noujeim [ 36 ] from Lebanon (1%). Higher prevalence of EPNs in soil from that determined in the present study was observed by Kanga [ 37 ] from Southern Cameroon (10.4%), Khatri-Chhetri [ 38 ] in Nepal (10.5%), and Malan [ 39 ] in South Africa (17%).…”

Section: Discussioncontrasting

confidence: 63%

A study on Xenorhabdus and Photorhabdus isolates from Northeastern Thailand: Identification, antibacterial activity, and association with entomopathogenic nematode hosts

et al. 2021

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Xenorhabdus and Photorhabdus are gram negative bacteria that can produce several secondary metabolites, including antimicrobial compounds. They have a symbiotic association with entomopathogenic nematodes (EPNs). The aim of this study was to isolate and identify Xenorhabdus and Photorhabdus species and their associated nematode symbionts from Northeastern region of Thailand. We also evaluated the antibacterial activity of these symbiotic bacteria. The recovery rate of EPNs was 7.82% (113/1445). A total of 62 Xenorhabdus and 51 Photorhabdus strains were isolated from the EPNs. Based on recA sequencing and phylogeny, Xenorhabdus isolates were identified as X. stockiae (n = 60), X. indica (n = 1) and X. eapokensis (n = 1). Photorhabdus isolates were identified as P. luminescens subsp. akhurstii (n = 29), P. luminescens subsp. hainanensis (n = 18), P. luminescens subsp. laumondii (n = 2), and P. asymbiotica subsp. australis (n = 2). The EPNs based on 28S rDNA and internal transcribed spacer (ITS) analysis were identified as Steinernema surkhetense (n = 35), S. sangi (n = 1), unidentified Steinernema (n = 1), Heterorhabditis indica (n = 39), H. baujardi (n = 1), and Heterorhabditis sp. SGmg3 (n = 3). Antibacterial activity showed that X. stockiae (bMSK7.5_TH) extract inhibited several antibiotic-resistant bacterial strains. To the best of our knowledge, this is the first report on mutualistic association between P. luminescens subsp. laumondii and Heterorhabditis sp. SGmg3. This study could act as a platform for future studies focusing on the discovery of novel antimicrobial compounds from these bacterial isolates.

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Section: Discussioncontrasting

confidence: 63%

A study on Xenorhabdus and Photorhabdus isolates from Northeastern Thailand: Identification, antibacterial activity, and association with entomopathogenic nematode hosts

et al. 2021

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“…A survey took place between 2015 and 2016 years, and soil samples were collected randomly with a hand shovel from different habitats such as forest, pasture, field crops, vegetable, and fruit orchards following rainy days in spring (March-June) and autumn (September-October) (Majić et al 2018). A total of 112 soil samples were collected.…”

Section: Methodsmentioning

confidence: 99%

Isolation, identification, and pathogenicity of entomopathogenic nematodes occurring in Cappadocia Region, Central Turkey

Yüksel

Canhilal

2019

Egypt J Biol Pest Control

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A survey for naturally occurring entomopathogenic nematodes (EPNs) was conducted in various agricultural fields in central Turkey, Nevsehir, between 2015 and 2016 years. EPNs were recovered from 20 of 112 soil samples (17.9%). Seventeen isolates were identified as Steinernema feltiae (Rhabditida: Steinernematidae). One was unknown Steinernema sp. (Rhabditida: Steinernematidae), and two were Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae). The most common species was S. feltiae, which was recovered from 5 out of 8 sites. The pathogenicity of these isolates was evaluated on the last larval instar of Galleria mellonella L. (Lepidoptera: Pyralidae) at different concentrations (25, 50, 100 IJs) in the laboratory at 25 ± 1°C. Maximum mortality rate (87%) was achieved from the H. bacteriophora AVB-15 isolate at the lowest concentration after 48 h post-inoculation, while the only isolate that caused maximum mortality of (100%) at the concentration of 50 IJs/ml, 24 h post-inoculation was S. feltiae DDKB-17 isolate. All isolates showed high pathogenicity on G. mellonella last instar ranging from 63 to 100% at the concentration of 100 IJs/ml, 48 h post-inoculation. The present survey revealed that these EPNs are commonly present at Nevsehir, and they might have a good potential in biological control of insect pests.

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“…In the present study, some characters such as the body length of IJs were relatively less than those described S. feltiae originally. Some other researchers had the same results (Campos-Herrera et al, 2006; Majić et al, 2018;Flores et al, 2021). It has been suggested that IJs body length is the longest when EPNs are raised at 8°C and this character could be limited at higher room temperature conditions (Hazir et al, 2001).…”

Section: Discussionmentioning

confidence: 56%

Steinernema Feltiae- Xenorhabdus Bovienii: More Information about this Bactohelminthic Complex from Iran

Kuhestani

Karimi

Makhdomi

2021

Preprint

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Background: Entomopathogenic nematodes (EPNs) of the families Steinernematidae and Heterorhabditidae that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria are one of the effective biological control agents of insect pests. Native isolates can probably be more efficacious to control insect pests than exotic ones due to their adaptability to indigenous environmental conditions. Results: In this study, Steinernema feltiae isolate FUM221 was recovered from soil samples collected from the Fandoghloo pasture, Ardabil province, Iran. Morphological investigations of the first and second-generation adults, infective juveniles, and molecular characterizations were given based on ITS and 18S rDNA genes. Besides, molecular analysis based on the 16S rRNA region and phenetic data recognized Xenorhabdus bovienii as its symbiont bacterium. The scanning electron microscopy (SEM) images verified the identification of this isolate.Conclusion: The molecular characterization using two loci and phylogenetic analyses provided more evidence for the classification of this steinernematid and its difference of the same species from other countries. Moreover, molecular and phenetic characterizations of its symbiotic bacterium were provided with low variations compared to other isolates. Herein, the comprehensive taxonomic data of this steinernematid from Iran is presented.

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First report of entomopathogenic nematode Steinernema feltiae (Rhabditida: Steinernematidae) from Croatia

Cited by 9 publications

References 26 publications

A study on Xenorhabdus and Photorhabdus isolates from Northeastern Thailand: Identification, antibacterial activity, and association with entomopathogenic nematode hosts

A study on Xenorhabdus and Photorhabdus isolates from Northeastern Thailand: Identification, antibacterial activity, and association with entomopathogenic nematode hosts

Isolation, identification, and pathogenicity of entomopathogenic nematodes occurring in Cappadocia Region, Central Turkey

Steinernema Feltiae- Xenorhabdus Bovienii: More Information about this Bactohelminthic Complex from Iran

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