First report of Enterocytozoon bieneusi infection on a pig farm in the Czech Republic

Sak, Bohumil; Kváč, Martin; Hanzlíková, Dagmar; Cama, Vitaliano

doi:10.1016/j.vetpar.2008.01.043

Cited by 73 publications

(55 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, no matter which gene was amplified, the prevalence of E. bieneusi in pigs was higher than that in any other study worldwide except one conducted in the Czech Republic, where up to 94% (74/79) of pigs were found to be infected with E. bieneusi (30). The prevalence rates of E. bieneusi in pigs are reported to be from 3.6% to 94.0% (Table 1).…”

Section: Discussionmentioning

confidence: 77%

“…Pigs are considered to be one of the most important reservoir hosts of E. bieneusi, and molecular epidemiological surveys have been conducted in 11 countries since the first report of E. bieneusi in pigs in Switzerland (20). Genotyping data for E. bieneusi isolates from pigs, including wild boars, have identified 34 genotypes, with 13 of them found in humans (Table 1) (17,(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31). Therefore, genotyping of E. bieneusi isolates from pigs will provide useful information to assess the risk of zoonotic transmission.…”

mentioning

confidence: 99%

“…The finding of the same ITS genotypes of E. bieneusi in China, eight in pigs and humans and four in pigs, wastewater, and humans, have raised public health concerns (Tables 2 and 3) (14,27,(31)(32)(33)(34)(35)(36), as the findings imply the potential for cross-genotype transmission from pigs to humans and the risk of exposure to environmental pathogens. Considering that E. bieneusi has been detected in healthy pigs (30), it is important to carry out a molecular epidemiological investigation of E. bieneusi in asymptomatic pigs, since inapparent infection may lead to less attention being paid to detecting zoonotic pathogens in these animals. Thus, pigs with no clinical signs may have more time and opportunity to continually shed human-infective spores through feces into the environment to spread the disease.…”

mentioning

confidence: 99%

See 2 more Smart Citations

High Prevalence of Enterocytozoon bieneusi in Asymptomatic Pigs and Assessment of Zoonotic Risk at the Genotype Level

Zhao

Zhang

Yang

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bEnterocytozoon bieneusi is an emerging and clinically significant enteric parasite infecting humans and animals and can cause life-threatening diarrhea in immunocompromised people. Pigs are considered to be one of the main reservoir hosts of E. bieneusi based on their high prevalence rates and zoonotic genotypes in pigs. As an opportunistic pathogen, E. bieneusi infection of pigs can be inapparent, which leads to neglect in detecting this parasite in pigs and assessing the epidemiological role of pigs in the transmission of human microsporidiosis. In the present study, 95 healthy pigs aged 2 or 3 months were randomly selected from three areas in Heilongjiang Province, China. E. bieneusi isolates were identified and genotyped based on the small-subunit (SSU) rRNA and internal transcribed spacer (ITS) regions of the rRNA gene by PCR and sequencing. A high prevalence of E. bieneusi was observed, 83.2% (79/95) at the SSU rRNA locus versus 89.5% (85/95) at the ITS locus. Ten ITS genotypes were obtained, comprising six known genotypes-EbpA (n ‫؍‬ 30), D (n ‫؍‬ 19), H (n ‫؍‬ 18), O (n ‫؍‬ 11), CS-1 (n ‫؍‬ 1), and LW1 (n ‫؍‬ 1)-and four novel genotypes named HLJ-I to HLJ-IV; 70.6% (60/85) of E. bieneusi genotypes were zoonotic (genotypes EbpA, D, and O). The findings of a high prevalence of E. bieneusi in pigs and a large percentage of zoonotic genotypes indicate that pigs may play a role in the transmission of E. bieneusi to humans and may become an important source of water contamination in our investigated areas.

show abstract

Section: Discussionmentioning

confidence: 77%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

High Prevalence of Enterocytozoon bieneusi in Asymptomatic Pigs and Assessment of Zoonotic Risk at the Genotype Level

Zhao

Zhang

Yang

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…To detect and identify species of Cryptosporidium, DNA from faecal samples was extracted as previously reported (Sak et al 2008) and nested-PCR protocols were used to amplify partial sequences of the Cryptosporidium small-subunit (SSU) rRNA gene according to Jiang et al (2005) and the Cryptosporidium 60-kDa glycoprotein gene (gp60) according to Li et al (2014). Amplicons of secondary PCR products were sequenced directly in both directions with an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).…”

Section: Methodsmentioning

confidence: 99%

Cryptosporidium ubiquitum, C. muris and Cryptosporidium deer genotype in wild cervids and caprines in the Czech Republic

Kotková¹,

Němejc²,

Sak³

et al. 2016

FOLIA PARASIT

Self Cite

View full text Add to dashboard Cite

A total of 269 faecal samples of various game animals, including 136 red deer (Cervus elaphus Linnaeus), 64 European fallow deer (Dama dama [Linnaeus]), 26 white-tailed deer (Odocoileus virginianus [Zimmermann]), and 43 mouflon sheep (Ovis orientalis musimon Pallas) were collected at 15 game preserves across the Czech Republic and examined for infection with species of Cryptosporidium Tyzzer, 1910 using microscopy (following aniline-carbol-methyl violet staining) and molecular tools. Oocysts of Cryptosporidium spp. were detected in one faecal sample originating from red deer. Ten positive cases of infection with cryptosporidia, including the case that was positive by microscopy, were detected using nested PCR. No associations between infection with cryptosporidia and diarrhoea were detected. Phylogenetic analyses based on the small subunit of the rRNA gene revealed the presence of three Cryptosporidium species/genotypes in ten positive samples: Cryptosporidium ubiquitum Fayer, Santín et Macarisin, 2010 was identified in five red deer, C. muris Tyzzer, 1907 in three samples (from a red deer, white-tailed deer and mouflon sheep), and Cryptosporidium deer genotype in two white-tailed deer. Subtyping of isolates of C. ubiquitum based on sequence analysis of the 60-kDa glycoprotein gene revealed that they belong to the XIId family. Finding C. muris and C. ubiquitum XIId for the first time in various wild cervids and caprines broadens their host range.

show abstract

“…DNA from E. intestinalis spores originally isolated from an AIDS patient (Didier et al 1996a) and grown in vitro in VERO E6 cells in the Laboratory of Veterinary and Medical Protistology at the institute of Parasitology of Ascr and from E. bieneusi spores of genotype D originally isolated from a pig (Sak et al 2008) was used as positive control.…”

Section: Methodsmentioning

confidence: 99%

Diversity of microsporidia (Fungi: Microsporidia) among captive great apes in European zoos and African sanctuaries: evidence for zoonotic transmission?

Sak¹,

Kváč²,

Petrželková³

et al. 2011

FOLIA PARASIT

Self Cite

View full text Add to dashboard Cite

Two hundred and seventeen captive great apes (150 chimpanzees, Pan troglodytes; 14 bonobos, Pan paniscus; 53 western gorillas, Gorilla gorilla) and 20 personnel from thirteen European zoos and two African sanctuaries were sampled and examined in order to determine the occurrence of Enterocytozoon bieneusi and species of Encephalitozoon in faecal specimens and to compare the epidemiological situation between zoos and sanctuaries. Microsporidia were detected at all sampling sites. Sequence analyses of ITS amplicons generated by using microsporidia-specific primers determined the presence of microsporidia in 87 samples including 13 humans; since two cases of simultaneous occurrence of Encephalitozoon cuniculi and Enterocytozoon bieneusi were identified, 89 full-length ITS sequences were obtained, namely 78 Encephalitozoon cuniculi genotype I, five E. cuniculi genotype ii, two E. hellem 1A and four Enterocytozoon bieneusi. No Encephalitozoon intestinalis-positive samples were identified. This is the first report of Encephalitozoon species and Enterocytozoon bieneusi genotypes in captive great apes kept under various conditions and the first record of natural infection with E. hellem in great apes. A comparison of zoos and sanctuaries showed a significantly higher prevalence of microsporidia in sanctuaries (P<0.001), raising a question about the factors affecting the occurrence of microsporidia in epidemiologically and sanitarily comparable types of facilities.

show abstract

First report of Enterocytozoon bieneusi infection on a pig farm in the Czech Republic

Cited by 73 publications

References 21 publications

High Prevalence of Enterocytozoon bieneusi in Asymptomatic Pigs and Assessment of Zoonotic Risk at the Genotype Level

High Prevalence of Enterocytozoon bieneusi in Asymptomatic Pigs and Assessment of Zoonotic Risk at the Genotype Level

Cryptosporidium ubiquitum, C. muris and Cryptosporidium deer genotype in wild cervids and caprines in the Czech Republic

Diversity of microsporidia (Fungi: Microsporidia) among captive great apes in European zoos and African sanctuaries: evidence for zoonotic transmission?

Contact Info

Product

Resources

About