First report of DMI‐insensitive <i>Cercospora beticola</i> on sugar beet in Ontario, Canada

Trueman, Cheryl L.; Hanson, Linda E.; Somohano, P.; Rosenzweig, N.

doi:10.5197/j.2044-0588.2017.036.020

Cited by 19 publications

(8 citation statements)

References 6 publications

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“…This finding indicates a shift towards resistance in the population typical of quantitative resistance demonstrated in other plant pathogens to demethylation inhibitors [67]. Resistance to demethylation inhibitor fungicides has also been reported in C. beticola populations found on sugar beet [68][69][70] including on sugar beet in Ontario, Canada [71], which shares high similarity to the C. beticola population in New York [59]. This suggests that long-term use and dependence on fungicides in this class as the central tenet of a disease management program is unsustainable.…”

Section: Diseases Affecting Foliar Health-cercospora Leaf Spotmentioning

confidence: 77%

Challenges and Prospects for Building Resilient Disease Management Strategies and Tactics for the New York Table Beet Industry

et al. 2018

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Abstract:The New York table beet industry is expanding and has unique challenges to minimize crop loss in both conventional and organic production. Diseases may reduce plant population density and increase heterogeneity in a stand, reduce the duration of time foliage is healthy, and decrease the yield of marketable roots. Rhizoctonia solani Kuhn and Pythium ultimum Trow are dominant in the pathogen complex affecting crop stand and root health. Cercospora leaf spot (CLS) caused by the fungus, Cercospora beticola Sacc

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Section: Diseases Affecting Foliar Health-cercospora Leaf Spotmentioning

confidence: 77%

Challenges and Prospects for Building Resilient Disease Management Strategies and Tactics for the New York Table Beet Industry

et al. 2018

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“…The use of resistant varieties and fungicides are important tools to control C. beticola infestation in field and the currently most important fungicides are MBCs, QoIs or DMIs 32 . According to FRAC, DMIs had been classified as medium risk fungicides and over the last decades a reduced efficacy against C. beticola has been reported 27,33,34 . Investigation and better understanding of the mechanisms, mediating this sensitivity reduction is necessary for an appropriate and sustainable disease and resistance management.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the EC 50 values against difenoconazole, all isolates were additionally grouped into three different sensitivity levels: high susceptibility (EC 50 < 0.01 μg mL −1 ), medium susceptibility (EC 50 0.011 to 1.0 μg mL −1 ) or low susceptibility (EC 50 > 1.0 μg mL −1 ). In addition to the German isolate Hevensen from 2008 and the US (09-32 and 10-74-4), 23 representative isolates were chosen for the analysis and further experiments, six isolates with high susceptibility (isolates 25,26,38,42,43,45), six with medium susceptibility (isolates 4, 6, 31, 37, 48) and eight with low susceptibility (isolates 5,12,14,17,24,29,33,34).…”

Section: Relation Of Cbcyp51 Amino Acid Alterations and Increased Ec mentioning

confidence: 99%

Evidence for the association of target‐site resistance in cyp51 with reduced DMI sensitivity in EuropeanCercospora beticola field isolates

Muellender¹,

Mahlein²,

Stammler³

et al. 2020

Pest Management Science

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BACKGROUND Cercospora leaf spot caused by Cercospora beticola is the most relevant foliar disease in sugar beet cultivation. In the last decade a decreasing sensitivity of C. beticola towards demethylation inhibitors (DMIs) occurred. Different mechanisms mediating a reduced sensitivity towards DMIs have been identified in different plant pathogens to date, such as target site mutations, over‐expression or active excretion of the fungicide. RESULTS A sequencing of the cytochrome P450‐dependent sterol 14α‐demethylase gene sequence (cyp51) of diverse C. beticola isolates collected in different European countries with reduced DMI sensitivity was performed in order to find a possible correlation of mutations with higher EC50 values. The amino acid alterations Y464S, L144F and I309T combined with L144F were found to be associated with a reduced sensitivity. Furthermore, mutations I387M, M145W and M145W with E460Q were found uniquely. Additionally, constitutive and fungicide triggered expression of cyp51 was assayed by means of RT‐qPCR. A very strong induction of cyp51 mRNA expression in sensitive isolates suggests that the fungal cells upregulate expression to maintain ergosterol biosynthesis in DMI presence. The less intensive cyp51 induction in isolates with higher EC50 values underlines the possible correlation of the found target‐site mutations with reduced sensitivity. CONCLUSION This study provides new results about possible alterations in the target gene mediating reduced sensitivity of C. beticola towards DMIs and hypothesized a fungicide induced over‐expression of the target enzyme CYP51 as natural reaction of the fungus to fungicide application. © 2020 Society of Chemical Industry

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“…Canada (Trueman et al, 2017), and the United States (Secor et al, 2010;Bolton et al, 2012b;Rosenzweig et al, 2020). Resistance to DMIs is observed as a near-continuum, ranging from high to low…”

Section: Frac Groupmentioning

confidence: 99%

Cercospora beticola: The intoxicating lifestyle of the leaf spot pathogen of sugar beet

Rangel

Spanner

Ebert

et al. 2020

Molecular Plant Pathology

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First report of DMI‐insensitive Cercospora beticola on sugar beet in Ontario, Canada

Cited by 19 publications

References 6 publications

Challenges and Prospects for Building Resilient Disease Management Strategies and Tactics for the New York Table Beet Industry

Challenges and Prospects for Building Resilient Disease Management Strategies and Tactics for the New York Table Beet Industry

Evidence for the association of target‐site resistance in cyp51 with reduced DMI sensitivity in EuropeanCercospora beticola field isolates

Cercospora beticola: The intoxicating lifestyle of the leaf spot pathogen of sugar beet

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