First report of cystic echinococcosis caused by Echinococcus granulosus sensu stricto/G1 in Felis catus from the Patagonian region of Argentina

Avila, Héctor Gabriel; Maglioco, Andrea; Gertiser, María Laura; Ferreyra, María Paula; Ferrari, Francisco; Klinger, Enrique; Venturi, Melisa Silvana Barbery; Agüero, Facundo Ariel; Fuchs, Alicia Graciela; Jensen, Oscar

doi:10.1007/s00436-021-07048-4

Cited by 5 publications

(8 citation statements)

References 17 publications

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“…Therefore, it can be suggested that CE may develop in immunocompromised cats. In many cases, the gender of the cat with hydatid cyst was determined as female (2,3,8,12). It was also seen in a female cat in our case.…”

Section: Türkiye'nin Hatay Ilinden Evcil Bir Kedide Renal Kistik Ekin...supporting

confidence: 49%

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Exploratory laparotomic diagnosis of renal cystic echinococcosis in a domestic cat from Hatay province of Türkiye and its molecular confirmation

ERDEM>

Zerek

Cura

et al. 2022

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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In this case report, in a twelve years old domestic cat is reported cystic echinococcosis which detected during exploratory laparotomy. In the anamnesis, there was a complaint of progressive abdominal swelling. As a result of clinical and radiological examinations, was detected unknown intraabdominal formations. At laparotomy were found to be multiple cysts on the right and left kidneys. As a result of molecular analysis, hydatid cyst, which is the larva of Echinococcus granulosus, was detected. This study is the first case of cystic echinococcosis detected molecularly in a domestic cat in Turkey.

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Section: Türkiye'nin Hatay Ilinden Evcil Bir Kedide Renal Kistik Ekin...supporting

confidence: 49%

“…Cysts were found either free inside the abdominal cavity (1,10) or attached to the liver (2,8,12) and spleen (3) in cats. In this case report localization of the cysts were kidneys.…”

Section: Türkiye'nin Hatay Ilinden Evcil Bir Kedide Renal Kistik Ekin...mentioning

confidence: 99%

Exploratory laparotomic diagnosis of renal cystic echinococcosis in a domestic cat from Hatay province of Türkiye and its molecular confirmation

ERDEM>

Zerek

Cura

et al. 2022

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…E. granulosus metacestode grows slowly and, probably, the cell cycle acceleration does not allow the protoscoleces to sprout and mature from the proliferative layer. However, this behavior could be responsible for the multiple sterile vesicles found in hosts, as described in Avila et al. (2021) .…”

Section: Discussionmentioning

confidence: 98%

“…The hermaphrodite worm is developed in the intestinal lumen of canids, which are the definitive hosts. Then, fertile proglottids containing oncospheres are shed into the soil by feces, and the intermediate hosts, ungulate animals, or aberrant hosts such as humans or cats ( Avila et al., 2021 ), acquire the parasite per os . The cycle closes when canids become infected by eating visceral organs from infected ungulate animals.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the B-Cell Epitopes of Echinococcus granulosus Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts

Maglioco

Agüero

Valacco

et al. 2022

Front. Cell. Infect. Microbiol.

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Cystic echinococcosis (CE) is a zoonotic disease worldwide distributed, caused by the cestode Echinococcus granulosus sensu lato (E. granulosus), with an incidence rate of 50/100,000 person/year and a high prevalence in humans of 5-10%. Serology has variable sensitivity and specificity and low predictive values. Antigens used are from the hydatid fluid and recombinant antigens have not demonstrated superiority over hydatid fluid. A cell line called EGPE was obtained from E. granulosus sensu lato G1 strain from bovine liver. Serum from CE patients recognizes protein extracts from EGPE cells with higher sensitivity than protein extracts from hydatid fluid. In the present study, EGPE cell protein extracts and supernatants from cell colonies were eluted from a protein G affinity column performed with sera from 11 CE patients. LC-MS/MS proteomic analysis of the eluted proteins identified four E. granulosus histones: one histone H4 in the cell extract and supernatant, one histone H2A only in the cell extract, and two histones H2A only in the supernatant. This differential distribution of histones could reflect different parasite viability stages regarding their role in gene transcription and silencing and could interact with host cells. Bioinformatics tools characterized the linear and conformational epitopes involved in antibody recognition. The three-dimensional structure of each histone was obtained by molecular modeling and validated by molecular dynamics simulation and PCR confirmed the presence of the epitopes in the parasite genome. The three histones H2A were very different and had a less conserved sequence than the histone H4. Comparison of the histones of E. granulosus with those of other organisms showed exclusive regions for E. granulosus. Since histones play a role in the host-parasite relationship they could be good candidates to improve the predictive value of serology in CE.

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“…Cats are the definitive hosts of E. multilocularis [ 9 ]. On the other hand, even though they can be an intermediate host for E. granulosus [ 10 11 ], they are not affected by the adult parasite because the parasite cannot resist stomach acids or attach to other parts of the digestive system [ 12 ]. This genus has ten genotypes (G1–G10) that affect different hosts [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

Al-Ardi

2024

J Vet Sci

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Background Infections of cats with Echinococcus granulosus is uncommon because the cat is not part of the parasite life cycle that a carnivorous and another herbivore represent. Nevertheless, it occurs incidentally when eating food or drinking water contaminated with the worm’s larva, especially with the presence of the definitive host (dogs), in this case, the infections are concentrated in stray or outside cats. For this reason, this study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection. Objective This study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection. Methods Four of the 37 cats that had died in different accidents developed cystic echinococcosis (CE). The cytochrome c oxidase subunit I (COX1) gene was initially amplified and sequenced to determine if these cysts belonged to E. granulosus, in beginning. The DNA fragments resulting from sequencing were then compared and aligned with other sequences using the Gene Bank database. Finally, a phylogenetic tree was drawn according to the sequence data obtained from cox1 genes sequencing, and the MEGA 7.0 phylogenetic analysis program was utilized. Results Four different sequences were deposited in the Gen Bank with accession numbers (ON795961 to ON795964), all of which belong to the G1 genotype. Approximately 84% and 100% of these sequences aligned with G1 (AB622277.1) and G1 (MG722980.1), respectively. Conclusions G1 is the dominant genotype that causes cat infections, even though the cat’s EC infection was incidental.

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First report of cystic echinococcosis caused by Echinococcus granulosus sensu stricto/G1 in Felis catus from the Patagonian region of Argentina

Cited by 5 publications

References 17 publications

Exploratory laparotomic diagnosis of renal cystic echinococcosis in a domestic cat from Hatay province of Türkiye and its molecular confirmation

Exploratory laparotomic diagnosis of renal cystic echinococcosis in a domestic cat from Hatay province of Türkiye and its molecular confirmation

Characterization of the B-Cell Epitopes of Echinococcus granulosus Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

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