First Report of Complete Mitochondrial Genome in the Tribes Coomaniellini and Dicercini (Coleoptera: Buprestidae) and Phylogenetic Implications

Abstract

The complete mitochondrial genomes (mitogenomes) of the tribes Coomaniellini and Dicercini were sequenced and described in this study, including Coomaniella copipes (16,196 bp), Coomaniella dentata (16,179 bp), and Dicerca corrugata (16,276 bp). These complete mitogenomes are very similar in length and encoded 37 typical mitochondrial genes, including 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs) and 13 protein-coding genes (PCGs). Most of PCGs had typical ATN start codons and terminated with TAR. Among these mitogenomes, Leu2 (L2), Ile (I), Ser2 (S2), and Phe (F) were the four most frequently encoded amino acids. Moreover, phylogenetic analyses were performed based on three kinds of nucleotide matrixes (13 PCGs, 2 rRNAs, and 13 PCGs + 2 rRNAs) among the available sequenced species of the family Buprestidae using Bayesian inference and Maximum-likelihood methods. The results showed that a Chrysochroninae species interspersed in Buprestinae, and Coomaniellini is more closely related to Dicercini than Melanophilini. Moreover, the clade of Buprestidae was well separated from outgroups and the monophyly of Agrilinae is confirmed again. Our whole mitogenome phylogenetic results support that the genus Dicerca can be transferred from Chrysochroinae to Buprestinae; whether Dicercini can be completely transferred remains to be further verified after enriching samples. Our results have produced new complete mitogenomic data, which will provide information for future phylogenetic and taxonomic research.

First mitochondrial genome of subfamily Julodinae (Coleoptera, Buprestidae) with its phylogenetic implications

“…The rRNA genes were located between the A + T-rich region and trnL1, and separated by trnV, which is consistent with previous studies (Duan et al 2017;Cao and Wang 2019a, b;Xiao et al 2019;Sun et al 2020;Chen et al 2021;Peng et al 2021;Huang et al 2022;Wei 2022;Weng et al 2022). The total length of rRNA genes ranged from 2035 bp (C. japonica) to 2093 bp (J. variolaris), of which the length of 16S gene ranged from 1299 bp (C. japonica and P. chinensis) to 1301 bp (J. variolaris).…”

“…In these three mitogenome, the N-strand encoded the sense-strand of 14 genes (nad1, nad4L, nad4, nad5, trnQ, trnV, trnL1, trnP, trnH, trnF, trnY, trnC, rrnL, and rrnS), while the J-strand encoded the sense-strand of the remaining 23 genes (Table 2), which was consistent with the known buprestid species (Cao and Wang 2019a, b;Xiao et al 2019;Chen et al 2021;Peng et al 2021;Huang et al 2022;Wei 2022;Weng et al 2022).…”

“…The UG mismatches were detected in some tRNAs (Suppl. material 1: figs S2-S4), which also appeared in other buprestid species (Sun et al 2020;Chen et al 2021;Huang et al 2022;Wei 2022;Weng et al 2022).…”

“…The majority of PCGs directly used ATN as the start codon, but the exceptions were nad1 (J. variolaris, P. chinensis, and C. japonica), nad4L (C. japonica), and nad5 (C. japonica) genes which started with TTG, GTG, and GTG, respectively. The unusual start codon TTG was also reported in Agrilinae (Wei 2022) and Buprestinae (Huang et al 2022). The start codon of the cox1 gene in these three mitogenomes was not determined, which may use non-canonical start codons (Friedrich and Muquim 2003;Fenn et al 2007;Yang et al 2013;Wang et al 2021;Wu et al 2022).…”

“…This region, containing regulatory elements correlated with the regulation of replication and transcription (Zhang et al 1995), plays a very important role in molecular evolution (Zhang and Hewitt 1997). The length of A + T-rich region ranged from 1080 bp (C. japonica) to 1510 bp (J. variolaris), which are of medium length in the Buprestidae (Sun et al 2020;Huang et al 2022;Wei 2022). The A + T content of the A + T-rich region of C. japonica (75.93%) and P. chinensis (78.38%) was found to be higher than that of the whole genome (67.97%, 67.00%), PCGs (66.46%, 64.55%), rRNAs (71.50%, 72.51%), and tRNAs (68.82%, 71.46%), whereas the A + T content of J. variolaris (72.85%) was lower than that of whole genome (70.43%), PCGs (68.82%), rRNAs (74.30%), and tRNAs (74.79%).…”