First report of “ Candidatus Rickettsia amblyommii” in west coast of Mexico

Sánchez‐Montes, Sokani; Ríos-Muñoz, César A.; Espinosa-Martínez, Deborah V.; Guzmán-Cornejo, Carmen; Berzunza-Cruz, Miriam; Becker, Ingeborg

doi:10.1016/j.ttbdis.2016.08.007

Cited by 25 publications

(8 citation statements)

References 70 publications

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“…In Mexico, R. amblyommatis has been detected in A. mixtum ( A. cajennense s.l.) detached from people [19] .…”

Section: Discussionmentioning

confidence: 99%

“…proposed the novel species name R. amblyommatis, which confirmed to the rules of the International Code of Nomenclature of Prokaryotes [4] . This bacterium is highly prevalent in A. americanum, and it has been also detected in other Amblyomma species throughout the Western Hemisphere as Amblyomma maculatum in the United States [5] , and Amblyomma auricularium, Amblyomma cajennense, Amblyomma coelebs, Amblyomma geayi, Amblyomma humerale, Amblyomma longirostre, Amblyomma mixtum, Amblyomma neummanni, Amblyomma hadanii, Amblyomma oblongoguttatum, Amblyomma ovale, Amblyomma sculptum and Amblyomma tonellidae in Central and South America [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] . Nowadays, several of these Amblyomma species are within A. cajennense s.l., because this taxon has been recently reassessed, including A. cajennense sensu stricto, A. mixtum, A. sculptum, Amblyomma interandinum, A. tonellidae and Amblyomma patinoi [20] .…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Rickettsia amblyommatis in HUVEC line

Santibáñez

Portillo

Palomar

et al. 2018

New Microbes and New Infections

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Rickettsia amblyommatis, formerly named Rickettsia amblyommii and ‘Candidatus Rickettsia amblyommii’ is an intracellular bacterium belonging to the spotted fever group Rickettsia. It is highly prevalent in Amblyomma americanum and in other Amblyomma spp. throughout the Western Hemisphere. R. amblyommatis has been cultivated in chicken fibroblast, primary embryonated chicken eggs, Vero cells and arthropod-derived cells. Because of the affinity of rickettsiae to invade vascular endothelial cells, we tried to isolate R. amblyommatis from a nymph of Amblyomma cajennense s.l. collected in Saltillo (Coahulia, Mexico) using human umbilical vein endothelial cells (HUVEC). One tick half was analysed by ompA PCR and was found to be positive for R. amblyommatis. The other half was selected for in vitro culture of Rickettsia spp. It was triturated in 1 mL of endothelial cell growth medium with 1% antibiotic–antimycotic solution, and the homogenate was inoculated into a HUVEC line. Culture was maintained at 33°C in endothelial cell growth medium plus 2 mM l-glutamine and 2% fetal calf serum, with 5% CO2. The medium was changed weekly. Culture was checked by Gimenez stain for Rickettsia-like intracellular organisms. After 48 days of incubation, Rickettsia-like organisms were observed in HUVEC. PCR assays and sequencing of ompA gene in the culture suspension showed 100% identity with R. amblyommatis. This isolate was successfully established in HUVEC, and it has been deposited in the collection of the Center of Rickettsioses and Arthropod-Borne Diseases, Infectious Diseases Department, Hospital San Pedro–Center of Biomedical Research from La Rioja, Logroño, Spain. The HUVEC line is a useful tool for the isolation of R. amblyommatis.

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“…In Mexico, R. amblyommatis has been detected in A. mixtum ( A. cajennense s.l.) detached from people [19] .…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of Rickettsia amblyommatis in HUVEC line

Santibáñez

Portillo

Palomar

et al. 2018

New Microbes and New Infections

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“…Further collaboration studies are required to evaluate and compare biological, ecological, and/or evolution factors that contribute to the increase of the possibility of R. sanguineus biting humans in north and south of Mexico. Other tick species ( A. mixtum ) infected by Rickettsia species such as Rickettsia amblyommatis have been identified by previous studies in the west coast of Mexico [ 13 ]. Amblyomma mixtum is important because it frequently bites humans [ 14 ] and is considered as the preferred vector of R. rickettsii in other countries in Latin America, commonly Brazil [ 15 – 18 ].…”

Section: Discussionmentioning

confidence: 99%

Presence of Rickettsia Species in a Marginalized Area of Yucatan, Mexico

Peniche-Lara

Jiménez-Delgadillo

Muñoz-Zanzi

et al. 2018

Journal of Tropical Medicine

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In the state of Yucatan, Mexico, rickettsiosis has become a common vector-borne disease in the general population. Ectoparasite species such as Rhipicephalus sanguineus and Amblyomma mixtum have been identified as Rickettsia vectors in Yucatan by studies focused on the wild animal population in rural areas. There have been studies that have tried to determine the presence of Rickettsia species in ectoparasites collected in Yucatan, but these studies did not include marginalized areas, where living in close contact with domestic and peridomestic animals that carry ectoparasites is a high-risk factor for acquiring rickettsial infection or many other vector-borne diseases. We evaluated the vector diversity and the presence of Rickettsia species presence in the ectoparasite population that parasitizes domestic animals in a marginalized rural town of Yucatan, Mexico; we also evaluated the seroprevalence of rickettsial antibodies in the human population of this town in order to determine the prevalence of rickettsial infection. A total of 437 ectoparasites were collected from the study area. The tick specimens collected belonged to the species Rhipicephalus sanguineus (n=380, 49 positive), Amblyomma mixtum (n=3, 0 positive), Ixodes affinis (n=4, 0 positive), Ctenocephalides felis (n=33, 0 positive), and Trichodectes canis (n=17, 0 positive). Conventional polymerase chain reaction and sequencing were used to identify the DNA of Rickettsia. Six out of 354 (1.8%) serum samples were positive for antibody to R. typhi. The combination of low antibody titers and the presence of Rickettsia species infecting ectoparasite species found in the study area requires eco-epidemiological studies and the identification of potentially protective practices or habits.

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“…It causes economic losses due to the large amount of blood taken from its hosts and the transmission of infectious diseases to domestic/wild animals (Anaplasma marginale) and humans (Rickettsia ricketsii) (Alonso- Díaz et al, 2013b;Aguilar-Domínguez et al, 2019). Additionally, other potentially zoonotic species such as Rickettsia amblyommatis have been detected in A. mixtum from Mexico (Sánchez-Montes et al, 2016;Merino et al, 2020), making this parasite one of the most important tick species in veterinary medicine and public health in the country (Pérez de León et al, 2020). The biological cycle of A. mixtum is shown in Figure 4.…”

Section: Amblyomma Mixtummentioning

confidence: 99%

Entomopathogenic Fungi for Tick Control in Cattle Livestock From Mexico

Alonso-Díaz

Fernández-Salas

2021

Front. Fungal Biol.

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Ticks are one of the main economic threats to the cattle industry worldwide affecting productivity, health and welfare. The need for alternative methods to control tick populations is prompted by the high prevalence of multiresistant tick strains to the main chemical acaricides and their ecological consequences. Biological control using entomopathogenic fungi (EPF) is one of the most promising alternative options. The objective of this paper is to review the use of EPF as an alternative control method against cattle ticks in Mexico. Metarhizium anisopliae sensu lato (s.l.) and Beauveria bassiana s.l. are the most studied EPF for the biological control of ticks in the laboratory and in the field, mainly against Rhipicephalus microplus; however, evaluations against other important cattle ticks such as Amblyomma mixtum and R. annulatus, are needed. A transdisciplinary approach is required to incorporate different types of tools, such as genomics, transcriptomics and proteomics in order to better understand the pathogenicity/virulence mechanism in EPF against ticks. Laboratory tests have demonstrated the EPF efficacy to control susceptible and resistant/multiresistant tick populations; whereas, field tests have shown satisfactory control efficiency of M. anisopliae s.l. against different stages of R. microplus when applied both on pasture and on cattle. Epidemiological aspects of ticks and environmental factors are considered as components that influence the acaricidal behavior of the EPF. Finally, considering all these aspects, some recommendations are proposed for the use of EPF in integrated control schemes for livestock ticks.

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First report of “ Candidatus Rickettsia amblyommii” in west coast of Mexico

Cited by 25 publications

References 70 publications

Isolation of Rickettsia amblyommatis in HUVEC line

Isolation of Rickettsia amblyommatis in HUVEC line

Presence of Rickettsia Species in a Marginalized Area of Yucatan, Mexico

Entomopathogenic Fungi for Tick Control in Cattle Livestock From Mexico

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