First report of ‘Candidatus Phytoplasma trifolii’ (16SrVI group) infecting cabbage (Brassica oleracea) in Turkey

Serçe, Çiğdem Ulubaş; Yılmaz, Salih

doi:10.1007/s42161-019-00443-y

Cited by 10 publications

(3 citation statements)

References 3 publications

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“…"Ca. P. trifolii" is also prevalent pathogen for various hosts in Asia, Europe and, American continents, including tomato in Jordan (Anfoka et al, 2003), pepper in Mexico (Reveles-Torres et al, 2018), grapevine, tomato, soybean, safflower in Iran (Shahryari et al, 2019;Ghayeb Zamharir and Aldaghi, 2018;Salehi et al, 2008;Davoodi et al, 2019), cabbage, sesame, periwinkle, eggplant, pepper in Turkey (Oksal et al, 2017;Ulubaş Serçe and Yılmaz, 2019;Özdemir, 2017;Sertkaya et al, 2007), in American Elm (Ulmus americana L.) in U.S.A (Flower et al, 2018), willow in China (Zhang et al, 2012), grapevine in Italy (Zambon et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch's broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the "Canditatus Phytoplasma solani" (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the "Canditatus Phytoplasma trifolii" (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as 'Bingöl D11' and 'Bingöl D90' isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong. This current paper is documented in the first record of "Ca. P. solani" (16SrXII-A) and "Ca. P. trifolii" (16SrVI-A) in naturally diseased tomato in Bingöl of Turkey.

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Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…In this study, we employed the 16S rRNA gene region, which is widely used in phytoplasma taxonomy. Although this sequence is valuable for revealing interspecies taxonomic levels, a multilocus analysis should be performed using the rp, secY, secA, cpn60, and tuf gene locus to distinguish closely related phytoplasma strains in the same 16Sr groups and gain advantages over single gene analysis (Davis et al, 2013;Dumonceaux et al, 2014;Serçe and Yılmaz 2019;Valiunas et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye

Usta

Güller

Sipahioğlu

2023

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye.

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“…'Candidatus Phytoplasma trifolii' have been previously reported in pears (Usta et al 2021), pepper (Yilmaz et al 2019, and cabbage (Serçe and Yılmaz 2019) in Turkey. Spirea spp.…”

Section: Main Textmentioning

confidence: 94%

First report of a 'Candidatus Phytoplasma trifolii'-related strain (16SrVI-A) associated with white leaf disease in Spirea japonica

Şimşek

Ayvaci

Akkurak

et al. 2021

Australasian Plant Dis. Notes

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In August of 2018 phytoplasmas naturally infecting Spirea japonica were found in Şanlıurfa province, Turkey and they were identified by nested polymerase chain reaction (PCR), restriction fragment length polymorphism analysis (RFLP) and phylogeny based on sequence analysis. Plants exhibited typical white leaf disease symptoms with reduced leaf size. The phytoplasmas were found to be closely related to 'Candidatus Phytoplasma trifolii' (16SrVI-A). This paper has reported that Spirea japonica is a new host for 'Ca. Phytoplasma trifolii' which is associated with the white leaf disease in S. japonica.

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First report of ‘Candidatus Phytoplasma trifolii’ (16SrVI group) infecting cabbage (Brassica oleracea) in Turkey

Cited by 10 publications

References 3 publications

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye

First report of a 'Candidatus Phytoplasma trifolii'-related strain (16SrVI-A) associated with white leaf disease in Spirea japonica

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