First report of Brenneria goodwinii causing acute oak decline on Quercus suber in Portugal

Fernandes, Clovis Torres; Duarte, Lídia; Naves, Pedro; Sousa, Edmundo; Cruz, Leonor

doi:10.1007/s42161-022-01046-w

Cited by 12 publications

(9 citation statements)

References 2 publications

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“…Furthermore, two isolates of B . goodwinii previously collected from cork oak in Portugal (CPBF 1752 and CPBF 1753) were also tested (Fernandes et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

“…PCRs for B. goodwinii (Hunter et al, 2013) were performed in a final volume of 25 μl with the following components: 5× Green GoTaq Flexi buffer, 25 mM MgCl 2 , 10 mM dNTPs, BG-F and BG-R at 100 μM (BG), 5 U GoTaq G2 Flexi DNA polymerase and 5 μl DNA template. Thermal and CPBF 1753) were also tested (Fernandes et al, 2022).…”

Section: Molecular Detection Of Known Pathogenic Strainsmentioning

confidence: 99%

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Bacterial community associated with the ambrosia beetle Platypus cylindrus on declining Quercus suber trees in the Alentejo region of Portugal

et al. 2022

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In recent decades, oak forests in Europe (Fagaceae family) have faced a severe multifactorial process of decline, characterized by a fast drying of the canopy and loss of tree vigour. Climate change and increased susceptibility to pests and diseases (e.g., the fungal pathogens Diplodia corticola and Phytophthora cinnamomi; Moricca et al., 2016) probably have a major role in this process (Sallé et al., 2014). These changes are registered worldwide, often without any precedents and with alarming outcomes (Hulcr & Dunn, 2011). In the Mediterranean area and mainly in Portugal, the evergreen Quercus suber (cork oak) woodlands have been showing this decline process since the 1980s, highly affecting the cork production (half of the world cork production is Portuguese; APCOR, 2020) and the biodiversity of the ecosystem. The symptoms include leaf discolouration, defoliation, small branches and sometimes exudates (Tiberi et al., 2016).

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“…Furthermore, two isolates of B . goodwinii previously collected from cork oak in Portugal (CPBF 1752 and CPBF 1753) were also tested (Fernandes et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Section: Molecular Detection Of Known Pathogenic Strainsmentioning

confidence: 99%

Bacterial community associated with the ambrosia beetle Platypus cylindrus on declining Quercus suber trees in the Alentejo region of Portugal

et al. 2022

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“…The main symptoms are bleeding bark cracks associated with insect galleries, crown thinning, and epicormic shoots, and death can occur within 4-5 years [10]. Since then, the presence of these pathogenic bacteria has been confirmed in many other countries, including Poland, Latvia, Portugal, Spain, and Switzerland [34][35][36][37]. Four bacteria are commonly recovered from bleeding cracks: G. quercinecans, B. goodwinii, R. victoriana, and L. britannica.…”

Section: Discussionmentioning

confidence: 99%

The Role of Bacteria in Acute Oak Decline in South-West Poland

Tkaczyk,

Sikora

2024

Microorganisms

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The process of multifactorial oak disease has been of interest to scientists from all over the world for many years. Recently, a new phenomenon has been added to the model related to oak decline, acute oak dieback, which causes oak decline and was first described in the UK. This study presents research on this phenomenon in the area of the largest oak stand complex in Poland, the so-called Krotoszyn Plateau. This work was carried out in two stages. In the first stage, 54 trees were tested for the presence of bacteria using molecular biology (real-time PCR). Subsequently, a tissue fragment was taken for inoculation from the trees in which the presence of B. goodwinii and G. quercinecans bacteria was confirmed. The isolates obtained were used to test Koch’s postulates and for biochemical analyses for Polish strains. In addition, the results obtained were also compared with the presence of A. biguttatus, which is considered a bacterial vector, which, in turn, confirmed that the bacteria responsible for the AOD phenomenon can also be present in trees not inhabited by this insect. The results obtained confirmed the presence of bacteria and their potential to cause necrosis in oaks, which fits into the model of the spiral disease that has been causing mass mortality of oaks in this naturally and economically valuable area since the 1980s.

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“…Tree decline characterized by bark necrosis and bleeding of a dark sticky fluid from vertical cracks on trunks and branches of affected trees was repeatedly reported in the United Kingdom, Iran, Spain and Portugal Bakhshi Ganje et al, 2020;Brady et al, 2010;Denman et al, 2012;Fernandes et al, 2022;González & Ciordia, 2020;Moradi-Amirabad et al, 2019).…”

Section: Introductionmentioning

confidence: 92%

Oriental beech decline caused by Brenneria alni

Mirhabibi,

Araeinejhad,

Falahi Charkhabi

2024

Forest Pathology

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Hyrcanian forests comprise a long strip along the southern coast of the Caspian Sea in northern Iran. Oriental beech (Fagus orientalis) is one of the most valuable forest tree species in the Hyrcanian forests. Bark necrosis and bleeding spots of oriental beech were observed in Savadkuh forests in Mazandaran province during summer 2021. Ten samples were collected from symptomatic oriental beech trees. The bark canker symptoms included stem tissue necrosis, dark brown to black spots on the trunk, bleeding with dark exudates and dark lesions in the inner bark. Twenty‐six strains of culturable bacteria were isolated from the samples which in culture were predominantly round, convex, smooth with entire margins on nutrient agar and a metallic green sheen pigment on eosin‐methylene blue (EMB) agar. Fourteen strains induced necrosis and browning of the tissues around the inoculation sites on acorns after 2 weeks. Five representative strains also caused dieback in twigs of oriental beech 3 weeks after inoculation. These bacteria were Gram‐negative, catalase positive and oxidase negative. All strains produced colonies with a green metallic sheen on EMB agar. Strains were negative in tests for starch and gelatine hydrolysis or production of levan. A phylogenetic tree based on concatenated sequences of gyrB and infB genes, of representative strains well as in each of the single gene‐based phylogenetic trees, indicated that these strains clustered with Brenneria alni NCPPB 3934T with high bootstrap values. To the best of our knowledge, this is the first report of B. alni associated with bark necrosis and bleeding spots on oriental beech.

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First report of Brenneria goodwinii causing acute oak decline on Quercus suber in Portugal

Cited by 12 publications

References 2 publications

Bacterial community associated with the ambrosia beetle Platypus cylindrus on declining Quercus suber trees in the Alentejo region of Portugal

Bacterial community associated with the ambrosia beetle Platypus cylindrus on declining Quercus suber trees in the Alentejo region of Portugal

The Role of Bacteria in Acute Oak Decline in South-West Poland

Oriental beech decline caused by Brenneria alni

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