First Report of Botrytis Bunch Rot of Grapes Caused by <i>Botrytis cinerea</i> in Pakistan

Javed, Sundus; Javaid, Arshad; Anwar, Waheed; Majeed, R. A.; Akhtar, Roma; Naqvi, Syeda Fakehha

doi:10.1094/pdis-05-16-0762-pdn

Cited by 11 publications

(8 citation statements)

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“…The identification of this specific fungus was viewed under a microscope for morphology characteristics. The morphological and molecular characteristics of B. cinerea were similar to the description of Javed et al [20] and Tijjani et al [3].…”

Section: Preparation Of B Cinereasupporting

confidence: 84%

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

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Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.

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Section: Preparation Of B Cinereasupporting

confidence: 84%

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

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“…Botrytis cinerea is a pathogen of grapevine causing Botrytis bunch rot and blight all over the world (Fournier et al 2013;Hyde et al 2014;Javed et al 2017). Cladosporium was also recorded in both approaches.…”

Section: Plant Pathogens and Endophytes In The Saprotrophic Fungal Comentioning

confidence: 99%

Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches

et al. 2018

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This study is unique as it compares traditional and high-resolution culture-independent approaches using the same set of samples to study the saprotrophic fungi on Vitis vinifera. We identified the saprotrophic communities of table grape (Red Globe) and wine grape (Carbanate Gernischet) in China using both traditional and culture-independent techniques. The traditional approach used direct observations based on morphology, single spore isolation and phylogenetic analysis yielding 45 taxa which 19 were commonly detected in both cultivars. The same set of samples were then used for Illumina sequencing which analyzed ITS1 sequence data and detected 226 fungal OTUs, of which 176 and 189 belong to the cultivars Carbanate Gernischet and Red Globe, respectively. There were 139 OTUs shared between the two V. vinifera cultivars and 37 and 50 OTUs were specific to Carbanate Gernischet and Red Globe cultivars respectively. In the Carbanate Gernischet cultivar, Ascomycota accounted for 77% of the OTUs and in Red Globe, almost all sequenced were Ascomycota. The fungal taxa overlap at the genus and species level between the traditional and culture-independent approach was relatively low. In the traditional approach we were able to identify the taxa to species level, while in the culture-independent method we were frequently able to identify the taxa to family or genus level. This is remarkable as we used the same set of samples collected in China for both approaches. We recommend the use of traditional techniques to accurately identify taxa. Culture-independent method can be used to get a better understanding about the organisms that are present in a host in its natural environment. We identified primary and secondary plant pathogens and endophytes in the saprotrophic fungal communities, which support previous observations, that dead plant material in grape vineyards can be the primary sources of disease. Finally, based on present and previous findings, we provide a worldwide checklist of 905 fungal taxa on Vitis species, which includes their mode of life and distribution.

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“…These morphological characteristics are similar to the description of Botrytis cinerea Pers. (Javed et al 2017). The primer pair ITS4/ITS5 of rDNA and part of glyceraldehyde-3-phosphate dehydrogenase gene (G3PDH) were used for amplification and sequencing of a representative isolate (BCH02).…”

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confidence: 99%

First report of gray mold rot disease on tomato (Solanum lycopersicum L.) caused by Botrytis cinerea in Malaysia

et al. 2018

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First Report of Botrytis Bunch Rot of Grapes Caused by Botrytis cinerea in Pakistan

Cited by 11 publications

References 0 publications

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches

First report of gray mold rot disease on tomato (Solanum lycopersicum L.) caused by Botrytis cinerea in Malaysia

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