First Report of Begonia Elatior Wilt Disease Caused by Fusarium foetens in France

Abstract

at room temperature for 24 h to induce sporulation. Morphological examination of conidia (45 to 169 x 15 to 21 nm) did not show significant differences from a known isolate of P. teres f. teres 0-1 (provided by Tim Friesen, ARS, Fargo, ND). For pathogenicity testing, six 14-day-old plants of barley cv. Tradition were sprayed until runoff with a 2,000 spore/ml suspension of two isolates from each location and the control P. teres f maculata isolate DEN2.6 (provided by Tim Friesen). Plants were incubated first in a lighted humidity chamber for 24 h and then in a greenhouse for 7 days at 21°C. Regardless of inoculum source, spot lesions surrounded by necrosis or chlorosis, typical of SFNB, appeared on the inoculated leaves within 7 days. Fungi isolated from symptomatic leaves were identified as P. teres and the morphology of the conidia was undistinguishable from those of P. teres f. teres. All control plants which were sprayed with sterile distilled water were symptomless. The pathogenicity test was repeated. Rapid PCR detection and amplicon sequencing (2) of the internal transcribed spacer (ITS) region of ribosomal genes was performed on field and pathogenicity test leaf lesion samples to confirm the presence of P. teres f maculata. DNA templates were prepared using the Extract-N-Amp Plant PCR Kits (Sigma Chemical Co., St. Louis, MO) and subjected to PCR using ITSl and ITS4 primers. Amplicons were then purified and sequenced. The 585-bp nucleotide sequences of P. teres í. maculata from Mon-Dak area were submitted to GenBank under accession nos. PtmNESl (JX187587), PtmSDYl (JX187588), PtmSDY2 (JX187589), and PtmWILl (JX 187590). The sequences from the four locations shared 100% similarity and also with P. teres f. maculata (EF452471) from GenBank while showing 10 nucleotide differences (99% similarity) with P. teres f teres (EF452472).The results represent first report of SFNB in the Mon-Dak. Barley is one of the most important crops in the area. Resistance of the NFNB and SFNB of barley are controlled by different genes (4). Based on this report, SFNB therefore have to be considered in selection of barley cultivars for cultivation in the area. References: (1) H. E Bockelman et al. Plant Dis. 67:696, 1983. (2) R. T. Lartey et al.

Fusarium commune associated with wilt and root rot disease in rice

“…In addition, F. oxysporum has caused root rot disease in soybean (Arias et al, 2013;Ellis et al, 2014), sweet potato (Clark and Moyer, 1988), wheat (Labuschagne et al, 2010), sugar beet (Hanson et al, 2018), pepper (Cerkauskas, 2017), and Amaranthus hybridus (Chen and Swart, 2000). Moreover, Fusarium foetens, another closely related species of F. oxysporum, caused wilt disease in Begonia elatior in France (Saurat et al, 2013). In this study, four F. commune isolates were obtained from diseased rice showing yellowing leaf symptoms.…”

“…Moreover, F usarium foetens , another closely related species of F . oxysporum , caused wilt disease in Begonia elatior in France (Saurat et al ., 2013). In this study, four F. commune isolates were obtained from diseased rice showing yellowing leaf symptoms.…”

Mechanisms of Surfactin from Bacillus subtilis SF1 against Fusarium foetens: A Novel Pathogen Inducing Potato Wilt

“…Fusarium foetens is a soil-borne plant pathogenic microorganism that was first reported to cause wilt in hybrid begonias in the Netherlands in 2000. These studies were rapidly followed by reports from Germany, England, and the USA in 2003, in France in 2007, and in Canada in 2010 [ 20 , 21 , 22 , 23 ]. F. foetens is relatively host-specific to begonia.…”