First Report of Bacterial Wilt Caused by <i>Ralstonia solanacearum</i> in Ornamental <i>Rosa</i> sp

Tjou-Tam-Sin, N. N. A.; Bilt, J. L. J. van de; Westenberg, Marcel; Bergsma-Vlami, M.; Korpershoek, H. J.; Vermunt, A.M.W.; Meekes, E.T.M.; Teunissen, Hedwich A. S.; Vaerenbergh, Johan Van

doi:10.1094/pdis-02-16-0250-pdn

Cited by 21 publications

(14 citation statements)

References 2 publications

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“…In 2014, a tropical variant of the pathogen belonging to R. pseudosolanacearum (Phylotype I) was found to be responsible for causing severe symptoms in rose planting material and cutting roses grown in glasshouses in the Netherlands (Tjou-Tam-Sin et al, 2017). This is the first description of bacterial wilt in rose and no information is currently available on the epidemiology of the pathogen in this particular host.…”

Section: Introductionmentioning

confidence: 99%

Survival of Ralstonia solanacearum and Ralstonia pseudosolanacearum in drain water

et al. 2018

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The survival in drain water of two strains of Ralstonia solanacearum and three strains of Ralstonia pseudosolanacearum, including two strains able to cause wilt in roses, was determined. Water draining from drip‐irrigated rock wool mats on which roses were grown was supplemented with the pathogen and survival was monitored at 4, 12, 20 and 28°C for up to 112 days. All strains were able to survive for at least 112 days in drain water at 12 and 20°C, but at 4°C maximum survival was 56 days. At 28°C, the survival period was strain dependent, but was at least 56 days. Populations declined gradually in non‐sterile drain water to a low level (maximum 100 cfu mL−1 after 112 days). In sterile drain water (autoclaved prior to addition of populations), no or only a limited decline in populations was found at 112 days, dependent on strain and temperature. Drain water that tested negative for Ralstonia in the dilution plating assay was tested for the presence of cells in a viable but non‐culturable state (VBNCs). Tomato plants were inoculated, but no symptoms developed, and plants sampled 22 days post‐inoculation were negative in a plating assay. Therefore, no indications were found that VBNCs were present.

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Section: Introductionmentioning

confidence: 99%

Survival of Ralstonia solanacearum and Ralstonia pseudosolanacearum in drain water

et al. 2018

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“…R. pseudosolanacearum strain PD7123 (IPO4001) isolated from a rose plant in the greenhouse (Tjou‐Tam‐Sin et al ., 2017) and R. solanacearum strain IPO1828 (PD2763) isolated from potato were used in this study. The isolates were grown on yeast extract peptone glucose agar (YPGA) medium (Sukroongreung et al ., 1984), incubated at 25°C for 72 h and checked for purity.…”

Section: Methodsmentioning

confidence: 99%

“…Recently in Europe, outbreaks of bacterial wilt in ornamental rose crops grown in glasshouses were described (Tjou‐Tam‐Sin et al ., 2017; Bergsma‐Vlami et al ., 2018). Rosa spp.…”

Section: Introductionmentioning

confidence: 99%

Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

et al. 2020

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The Ralstonia solanacearum species complex (RSSC) can cause bacterial wilt in a wide variety of plant species, including a number of ornamental glasshouse crops. Recently in Europe, ornamental rose plants for the production of cut flowers and propagation materials have been strongly affected by Ralstonia pseudosolanacearum, phylotype I, biovar 3. To test for the presence of the pathogen in the glasshouse, sampling of water from a drainage gutter or well may be an efficient strategy since it is known that RSSC can be released from infected root systems in the water. A protocol was developed to detect low densities of R. pseudosolanacearum in drain water collected from rose growers. Drain water was filtered through a bacterial filter, the filtrate was collected and target bacteria enriched for 48 h in Semi‐selective Medium South Africa (SMSA) broth supplemented with sterilized tomato plant extracts. DNA extracted from the enrichment broth was analysed using a TaqMan test in a duplex format, based on specific egl sequences of RSSC and the use of an extraction and amplification control. The optimized protocol had a detection level of ≤1–10 colony forming units of R. pseudosolanacearum in drain water.

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“…). Typical symptoms following heavy infections of R. pseudosolanacearum (Phylotype I) include necrosis of the stems and intense brown discoloration at the stem base (Tjou‐Tam‐Sin et al ., ) (Fig. ).…”

Section: Detectionmentioning

confidence: 93%

“…Some of these strains have occasionally been introduced into the EPPO region with ornamental/herbal plants or plant parts of tropical origin and can cause disease under greenhouse conditions in temperate climates, for example in Curcuma longa (turmeric), Anthurium , Epipremnum or more recently Rosa spp. (Tjou‐Tam‐Sin et al ., ). Within R. pseudosolanacearum , some strains with specific sequevars affect Zingiber (ginger) and Morus (mulberry).…”

Section: Introductionmentioning

confidence: 97%

PM 7/21 (2) Ralstonia solanacearum, R. pseudosolanacearum and R. syzygii (Ralstonia solanacearum species complex)

2018

EPPO Bulletin

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Diagnostics Diagnostic PM 7/21 (2) Ralstonia solanacearum, R. pseudosolanacearum and R. syzygii (Ralstonia solanacearum species complex) Specific scope This Standard describes a diagnostic protocol for Ralstonia solanacearum, Ralstonia pseudosolanacearum and Ralstonia syzygii, i.e. phylotype/sequevar strain in the Ralstonia solanacearum Species Complex (RSSC). 1 It should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols.

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First Report of Bacterial Wilt Caused by Ralstonia solanacearum in Ornamental Rosa sp

Cited by 21 publications

References 2 publications

Survival of Ralstonia solanacearum and Ralstonia pseudosolanacearum in drain water

Survival of Ralstonia solanacearum and Ralstonia pseudosolanacearum in drain water

Detection of Ralstonia pseudosolanacearum in drain water based on concentration, enrichment and the use of a duplex TaqMan PCR test

PM 7/21 (2) Ralstonia solanacearum, R. pseudosolanacearum and R. syzygii (Ralstonia solanacearum species complex)

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