First Report of Bacterial Soft Rot of White Flowered Calla Lily Caused by <i>Erwinia chrysanthemi</i> in Taiwan

Lee, Yung-An; Chen, K.-P.; Chang, Yuan-Chi

doi:10.1094/pdis.2002.86.11.1273a

Cited by 17 publications

(9 citation statements)

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“…Blast search with 16S sequences in groups I and III in GenBank did not reveal identical sequences. The sequences that resembled group I sequences most were from strain 580, isolated from Syngonium podophyllum (AF373202, Fessehaie et al 2002) and S3-1, isolated from Zantedeschia aethiopica (AY360397, Lee and Chen 2002), both ornamentals in the Araceae. The two strains originating from potato in the 16S tree, 340 and 571 (Fessehaie at al.…”

Section: Discussionmentioning

confidence: 98%

Characterization of Dickeya strains isolated from potato and river water samples in Finland

et al. 2008

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Plant pathogenic enterobacteria in the genera Pectobacterium and Dickeya (formerly classified as Erwinia) were isolated from diseased potato stems and tubers. The isolated bacteria were identified as P. atrosepticum, P. carotovorum and pathogens in the genus Dickeya with PCR tests. Furthermore, Dickeya strains were isolated from river water samples throughout the country. Phylogenetic analysis with 16S-23S rDNA intergenic spacer sequences suggested that the Dickeya strains could be divided into three groups, two of which were isolated from potato samples. Phylogenetic analysis with 16S rDNA sequences and growth at 39°C suggested that one of the groups corresponds to D. dianthicola, a quarantine pathogen in greenhouse cultivation of ornamentals, while two of the groups did not clearly resemble any of the previously characterised Dickeya species. Field trials with the strains indicated that D. dianthicolalike strains isolated from river samples caused the highest incidence of rotting and necrosis of potato stems, but some of the Dickeya strains isolated from potato samples also caused symptoms. The results showed that although P. atrosepticum is still the major cause of blackleg in Finland, virulent Dickeya strains were commonly present in potato stocks and rivers. This is the first report suggesting that Dickeya, originally known as a pathogen in tropical and warm climates, may cause diseases in potato in northern Europe.

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Section: Discussionmentioning

confidence: 98%

Characterization of Dickeya strains isolated from potato and river water samples in Finland

et al. 2008

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“…This species was first detected causing stunting and slow wilting of Dianthus in the early 1950s in Denmark, the Netherlands and the UK (Hellmers, 1958). Dickeya dianthicola was subsequently listed as a quarantine organism (EPPO A‐2) on Dianthus (Council Directive 2000/29/EC), but has also been found to cause disease on potato, tomato, chicory and artichoke, as well as on ornamental genera such as Begonia , Dahlia , Freesia , Hyacinthus , Iris , Kalanchoe and Zantedeschia (Samson & Nassan‐Agha, 1978; Dickey, 1979; Janse & Ruissen, 1988; Lee et al. , 2002; Samson et al.…”

Section: Dickeya Species On Potatomentioning

confidence: 99%

Dickeya species: an emerging problem for potato production in Europe

Toth¹,

Wolf²,

et al. 2011

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Dickeya species (formerly Erwinia chrysanthemi) cause diseases on numerous crop and ornamental plants world‐wide. Dickeya spp. (probably D. dianthicola) were first reported on potato in the Netherlands in the 1970s and have since been detected in many other European countries. However, since 2004–5 a new pathogen, with the proposed name ‘D. solani’, has been spreading across Europe via trade in seed tubers and is causing increasing economic losses. Although disease symptoms are often indistinguishable from those of the more established blackleg pathogen Pectobacterium spp., Dickeya spp. can initiate disease from lower inoculum levels, have a greater ability to spread through the plant’s vascular tissue, are considerably more aggressive, and have higher optimal temperatures for disease development (the latter potentially leading to increased disease problems as Europe’s climate warms). However, they also appear to be less hardy than Pectobacterium spp. in soil and other environments outside the plant. Scotland is currently the only country in Europe to enforce zero tolerance for Dickeya spp. in its potato crop in an attempt to keep its seed tuber industry free from disease. However, there are a number of other ways to control the disease, including seed tuber certification, on‐farm methods and the use of diagnostics. For diagnostics, new genomics‐based approaches are now being employed to develop D. dianthicola‐ and ‘D. solani’‐specific PCR‐based tests for rapid detection and identification. It is hoped that these diagnostics, together with other aspects of ongoing research, will provide invaluable tools and information for controlling this serious threat to potato production.

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“…White‐flowered calla lily isolates had a distinct pattern and could easily be distinguished from other E. chrysanthemi isolates from different plant hosts in Taiwan, suggesting that the former may not be indigenous to Taiwan. White‐flowered calla lilies have only been grown in Taiwan for a few years and the soft‐rot disease was observed only recently (Lee et al ., 2002). Thus, the pathogen may have been introduced through infected bulbs from another country.…”

Section: Discussionmentioning

confidence: 99%

“…The disease is characterized by foul‐smelling rot and collapse of the flower stems, and was found in some nurseries in the Yang Ming Shan area, Taipei, Taiwan (Lee et al ., 2002). The bacterial pathogen was identified as Erwinia chrysanthemi using physiological and biochemical features and sequences of the 16S ribosomal RNA gene (Lee et al ., 2002): E. chrysanthemi was earlier reported on other crops in Taiwan (Huang & Lee, 1988; Chuang et al ., 1989; Chung et al ., 1992; Lee et al ., 2002). Thus, isolates of E. chrysanthemi from white‐flowered calla lily may be indigenous to Taiwan and/or they may have been introduced through infected bulbs from other countries.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Erwinia chrysanthemi, the soft‐rot pathogen of white‐flowered calla lily, based on pathogenicity and PCR‐RFLP and PFGE analyses

Lee¹,

Chen

Hsu

2006

Plant Pathology

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Isolates of Erwinia chrysanthemi from Zantedeschia aethiopica (white-flowered calla lily) induced symptoms of soft rot on inoculated cv. Innocence flower-stem segments. Isolates from Phalaenopsis aphrodite and potato caused mild symptoms, while those from green onion and celery produced no symptoms. In addition to pathogenicity, the isolates were further characterized at the molecular level. A specific oligonucleotide primer set was designed for the detection of the pelZ gene of E. chrysanthemi . All E. chrysanthemi isolates tested contained pelZ as determined by PCR amplification. No amplification was observed with other Erwinia spp. The pelZ of E. chrysanthemi isolate S3-1 from Z. aethiopica was cloned, sequenced and compared with the nucleotide sequence of pelZ in GenBank. A point mutation produced an Ahd I restriction site, leading to the development of a PCR-RFLP assay to discriminate white-flowered calla lily isolates from others of E. chrysanthemi . Furthermore, macrorestriction analysis by modifying a pulsed-field gel electrophoresis (PFGE) protocol used by PulseNet revealed the genomic variation within E. chrysanthemi . After digestion with the restriction enzyme Xba I, white-flowered calla lily E. chrysanthemi isolates could be easily distinguished from other isolates. Differences in virulence, combined with PCR-RFLP and PFGE analyses, suggest that white-flowered calla lily E. chrysanthemi isolates are a new strain or pathotype in Taiwan.

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First Report of Bacterial Soft Rot of White Flowered Calla Lily Caused by Erwinia chrysanthemi in Taiwan

Cited by 17 publications

References 0 publications

Characterization of Dickeya strains isolated from potato and river water samples in Finland

Characterization of Dickeya strains isolated from potato and river water samples in Finland

Dickeya species: an emerging problem for potato production in Europe

Characterization of Erwinia chrysanthemi, the soft‐rot pathogen of white‐flowered calla lily, based on pathogenicity and PCR‐RFLP and PFGE analyses

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