First report of bacterial leaf blight of white‐flowered calla lily caused by <i>Xanthomonas campestris</i> pv. <i>zantedeschiae</i> in Taiwan

Lee, Yung-An; Chen, K.-P.; Chang, Yuan-Chi

doi:10.1111/j.1365-3059.2005.01168.x

Cited by 6 publications

(4 citation statements)

References 1 publication

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“…Bacterial leaf spot of this oilseed crop was also described in India (23,104), and it would be interesting to type Indian isolates to determine their genetic relatedness to the African isolates. Within pathovar zantedeschiae, the two strains that originated in South Africa, where the disease was first described (24), did not cluster with the strain that originated in Taiwan (73). Based on seven housekeeping genes and using a comprehensive collection of X. arboricola strains, we confirmed that strains of X. campestris pv.…”

Section: Discussionsupporting

confidence: 59%

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Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing

Saux

Bonneau

Essakhi

et al. 2015

Appl Environ Microbiol

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cDeep and comprehensive knowledge of the genetic structure of pathogenic species is the cornerstone on which the design of precise molecular diagnostic tools is built. Xanthomonas arboricola is divided into pathovars, some of which are classified as quarantine organisms in many countries and are responsible for diseases on nut and stone fruit trees that have emerged worldwide. Recent taxonomic studies of the genus Xanthomonas showed that strains isolated from other hosts should be classified in X. arboricola, extending the host range of the species. To investigate the genetic structure of X. arboricola and the genetic relationships between highly pathogenic strains and strains apparently not relevant to plant health, we conducted multilocus sequence analyses on a collection of strains representative of the known diversity of the species. Most of the pathovars were clustered in separate monophyletic groups. The pathovars pruni, corylina, and juglandis, responsible for pandemics in specific hosts, were highly phylogenetically related and clustered in three distinct clonal complexes. In contrast, strains with no or uncertain pathogenicity were represented by numerous unrelated singletons scattered in the phylogenic tree. Depending on the pathovar, intraand interspecies recombination played contrasting roles in generating nucleotide polymorphism. This work provides a population genetics framework for molecular epidemiological surveys of emerging plant pathogens within X. arboricola. Based on our results, we propose to reclassify three former pathovars of Xanthomonas campestris as X. arboricola pv. arracaciae comb. nov., X. arboricola pv. guizotiae comb. nov., and X. arboricola pv. zantedeschiae comb. nov. An emended description of X. arboricola Vauterin et al. 1995 is provided.A s international trade and travel increase, emerging infectious diseases regularly threaten human health, as well as agricultural production (livestock and crops), and may be responsible for huge social, economic, and environmental damages. Our capability to quickly detect and identify the microorganisms responsible for these emerging diseases is critical to implement effective crop protection, sanitary measures, and regulations. The development of efficient and precise diagnostic tools relies on a stable and comprehensive classification. Providing a classification frame with names predictive of ecological, phenotypic, genotypic, and phylogenetic properties is the primary aim of taxonomic studies.Sequencing of sets of housekeeping genes (multilocus sequence analysis [MLSA]) has become the standard today for phylogenetic analyses of bacterial species and was proposed as an alternative to DNA-DNA hybridizations for species delineation (1, 2). MLSA allows strain assignment at the species level (3-5) and gives rise to specialized databases (5-7). MLSA treats sequences at the nucleotide level for phylogenetic analysis, but the recorded data can also be converted into alleles, an approach known as multilocus sequence typing (MLST). MLST was introdu...

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Section: Discussionsupporting

confidence: 59%

“…The pathovar zantedeschiae is also a seldom-recorded pathovar (72,73). The strain from Taiwan did not cluster with the original strains isolated in South Africa.…”

Section: Discussionmentioning

confidence: 99%

Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing

Saux

Bonneau

Essakhi

et al. 2015

Appl Environ Microbiol

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“…zantedeschiae (Joubert and Truter, 1972). This pathovar was also later found in Taiwan (Lee et al., 2005).…”

Section: Introductionsupporting

confidence: 58%

Pectolytic Bacteria Associated with Soft Rot of Calla Lily (Zantedeschiaspp.) Tubers

Mikiciński¹,

Sobiczewski²,

Sulikowska³

et al. 2010

Journal of Phytopathology

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Soft rot is the most important disease on calla lily in Poland. The isolation of the presumptive pathogen from symptomatic tubers on nutrient agar yielded bacteria with different colony morphology. Of 41 isolates collected, 10 showed pectolytic activity on crystal violet pectate medium and caused soft rot on potato slices. All pectolytic bacteria appeared to be Gram-negative rods producing typical soft rot on inoculated leaf petioles of calla lily. Bacteria with colonies which morphologically resembled those used for inoculation were re-isolated from diseased petioles. Their identification was based on phenotypic characters and sequence of the gene fragment coding 16S rRNA. It was found that, in addition to Pectobacterium carotovorum subsp. carotovorum, soft rot of calla lily can be caused by Pectobacterium carotovorum subsp. atrosepticum, Pseudomonas marginalis, Pseudomonas veronii and Chryseobacterium indologenes. The latter two are described for the first time as plant pathogens. The pectolytic activity of all identified bacteria, except that of P. carotovorum subsp. atrosepticum, was lower than that of P. carotovorum subsp. carotovorum, but strains of P. veronii showed a higher activity than P. marginalis and C. indologenes species.

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“…poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), cause severe bulb and flower diseases in different ornamental plants and agriculturally important crops, like poinsettia, calla lily, hyacinth and some closely related bulb plants ( Janse and Miller, 1983 ; Joubert and Truter, 1972 ; Lee et al, 2005b ). Diseases caused by these pathogens are easily spread in the field by wind and rain.…”

mentioning

confidence: 99%

Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences

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Lee

et al. 2015

The Plant Pathology Journal

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In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of 1 pg/μl per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases.

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First report of bacterial leaf blight of white‐flowered calla lily caused by Xanthomonas campestris pv. zantedeschiae in Taiwan

Cited by 6 publications

References 1 publication

Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing

Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing

Pectolytic Bacteria Associated with Soft Rot of Calla Lily (Zantedeschiaspp.) Tubers

Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences

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