First report of bacterial blight of anthurium caused by <i>Xanthomonas axonopodis</i> pv. <i>dieffenbachiae</i> in Turkey

Aysan, Y.; Şahın, Fikrettin

doi:10.1111/j.1365-3059.2003.00892.x

Cited by 16 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…dieffenbachiae. ABB was first described in Hawaii (18) and subsequently has been found in the different producing regions, including Venezuela (16), Brazil (9), California (10), the Caribbean (34), the Philippines (24), Florida (29), Tahiti (23), and, more recently, Réunion Island (36) and Turkey (4). It has also been found sporadically in The Netherlands (the largest producer in the world) and Italy (35,40).…”

mentioning

confidence: 99%

Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium ( Anthurium andreanum ) Tissues by Nested PCR

Robène-Soustrade

Laurent

Gagnevin

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequencecharacterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to anthurium tested and for isolates originating from syngonium and not pathogenic to anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (10 3 CFU ml ؊1 ) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.

show abstract

mentioning

confidence: 99%

Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium ( Anthurium andreanum ) Tissues by Nested PCR

Robène-Soustrade

Laurent

Gagnevin

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Outbreaks or incursions of the pathogen in association with Anthurium were recurrently recorded in Europe since the 1990s, e.g. in the Netherlands (Sathyanarayana et al., 1998), Italy (Zoina et al., 2000), Turkey (Aysan & Sahin, 2003), Germany (Moltmann, 2005), Romania (Vlad et al., 2004) and Poland (Puławska et al., 2008). It is present in various countries of Central, South and North America, in Oceania, South Africa and Asia (Jouen et al., 2007; Robene‐Soustrade et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

PM 7/23 (3) Xanthomonas phaseoli pv. dieffenbachiae

2023

EPPO Bulletin

View full text Add to dashboard Cite

Specific scope: This Standard describes a diagnostic protocol for Xanthomonas phaseoli pv. dieffenbachiae. 1 This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Specific approval and amendment: First approved in 2003-09. Revised in 2009-09 and in 2023-08. 1Use of brand names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable.

show abstract

“…2000), New Caledonia (Jouen et al. 2007), Turkey (Aysan and Sahin 2003). Outbreaks or incursions of the pathogen were repeatedly recorded in Europe in association with anthurium during the last decade (Sathyanarayana et al.…”

Section: Introductionmentioning

confidence: 99%

A new semi-selective medium for the isolation ofXanthomonas axonopodispv.dieffenbachiae, the etiological agent of anthurium bacterial blight

Laurent

Chabirand²,

Jouen

et al. 2009

Letters in Applied Microbiology

View full text Add to dashboard Cite

A new semi-selective medium for the isolation of Xanthomonas axonopodis pv. dieffenbachiae, the etiological agent of anthurium bacterial blight . On anthurium, leaf symptoms start as water-soaked spots visible near the margins which often become surrounded by yellowish areas. The pathogen moves into vascular tissues (and infected stems turn dark brown), eventually leading to death of the plant. Blight symptoms can be observed on spathes. X. a. dieffenbachiae is able to infect a broad range of aroids and the strains display a complex host range specificity (Chase et al. 1992). The Keywords anthurium, CS medium, ET medium, NCTM4 medium, semi-selective isolation, Xanthomonas axonopodis pv. dieffenbachiae. AbstractAims: Xanthomonas axonopodis pv. dieffenbachiae causes anthurium blight, which is regarded as the most threatening disease for the anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-CephalexinTrimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results: A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and EsculinTrehalose (ET) media. The strain collection included: the anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of anthurium; and representatives of bacteria that are commonly present in the anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion: Growth of the anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study: This study will lead to improved isolation protocols of the anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.

show abstract

First report of bacterial blight of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae in Turkey

Cited by 16 publications

References 3 publications

Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium ( Anthurium andreanum ) Tissues by Nested PCR

Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium ( Anthurium andreanum ) Tissues by Nested PCR

PM 7/23 (3) Xanthomonas phaseoli pv. dieffenbachiae

A new semi-selective medium for the isolation ofXanthomonas axonopodispv.dieffenbachiae, the etiological agent of anthurium bacterial blight

Contact Info

Product

Resources

About