First Report of Association of a ‘<i>Candidatus</i> Phytoplasma aurantifolia’ 16SrII-D Related Phytoplasma with Geranium (<i>Pelargonium hortorum</i>) Little Leaf Disease in Iran

Amirmijani, Amirreza; Salari, Mohammadreza; Khoshsohbat, M. S.; Azadvar, Mehdi

doi:10.1094/pdis-03-20-0561-pdn

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Albizia lebbeck, and Pelargonium hortorum in the southern part of Kerman province (Amirmijani et al, 2020;Salari & Azadvar, 2019). In addition, the plants Vicia faba and Punica granatum in Fars province (Iran's southwest) (Salehi, Esmaeilzadeh-Hosseini, et al, 2016;Salehi, Rasoulpour, & Izadpanah, 2016), as well as Petroselinum crispum and Heliopsis helianthoides in the country's central region (Yazd province) (Babaei et al, 2022;Salehi, Esmailzadeh Hosseini, et al, 2016) have been reported to be infected by the same phytoplasma subgroup strains.…”

Section: Discussionmentioning

confidence: 99%

“…The 16SrII‐D subgroup strains were also indicated to cause diseases in other plants in Iran, including Capsicum annuum , Zinnia elegans , and Artemisia sieberi in the south of the country (Hormozgan province, Faghihi et al, 2016; Hemmati & Nikooei, 2017, 2019), Albizia lebbeck , and Pelargonium hortorum in the southern part of Kerman province (Amirmijani et al, 2020; Salari & Azadvar, 2019). In addition, the plants Vicia faba and Punica granatum in Fars province (Iran's southwest) (Salehi, Esmaeilzadeh‐Hosseini, et al, 2016; Salehi, Rasoulpour, & Izadpanah, 2016), as well as Petroselinum crispum and Heliopsis helianthoides in the country's central region (Yazd province) (Babaei et al, 2022; Salehi, Esmailzadeh Hosseini, et al, 2016) have been reported to be infected by the same phytoplasma subgroup strains.…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

Movahedi‐Parizi

Hosseinipour

Azadvar

et al. 2022

Annals of Applied Biology

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Given the potential for urban green spaces to provide fresh and healthy environments for humans, exploring the issues that threaten plants in these places is crucial.Phytoplasma-related symptoms were encountered on some plants in urban green spaces in the province of Kerman, southeastern Iran, between 2017 and 2019. Affected periwinkles and petunias exhibited phytoplasma disease symptoms, including virescence, phyllody, and witches'-broom. However, ball or disc-like shoot proliferation symptoms were noticed on the trunks and branches of pine trees. PCR was performed with phytoplasma-detecting universal primers, targetting and amplifying the 16S rRNA gene, and determining whether phytoplasmas are implicated in the symptomatic plants. The infection of the symptomatic plants was confirmed using nested-PCR amplification of expected DNA sizes for phytoplasmas. No product, however, was amplified from sampled symptomless plants. The sequencing of nested-PCR products was performed to obtain sequences encasing the standard F2nR2 fragments. The resulted sequences were submitted to iPhyClassifier, the universal phytoplasma classification platform, for the taxonomic assignment of the found phytoplasmas compared with previously identified 'Candidatus Phytoplasma' species, groups, and subgroups. The results revealed that phytoplasma strains related to the species 'Ca. P. trifolii' (16SrVI-A subgroup) infect periwinkles and pines. However, strains from the species 'Ca. P. aurantifolia' (16SrII-D subgroup) and 'Ca. P. phoenicium' (16SrIX-C subgroup) were found in petunias and periwinkles, respectively. To the best of our knowledge, phytoplasmas from the 16SrVI-A and 16SrII-D subgroups are the first reported to infect these plants in Kerman province, while a related strain from the subgroup 16SrIX-C is the first recorded to infect periwinkles in Iran and the second in the world.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

Movahedi‐Parizi

Hosseinipour

Azadvar

et al. 2022

Annals of Applied Biology

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Phytoplasma aurantifolia (lime witches' broom phytoplasma)

2022

CABI Compendium

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The ‘Candidatus Phytoplasma ziziphi’ effectors SJP1/2 negatively control leaf size by stabilizing the transcription factor ZjTCP2 in jujube

Ma,

Zheng,

Zhang

et al. 2024

Journal of Experimental Botany

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Phytoplasmas manipulate host plant development to benefit their invasion and insect vector colonization. However, the virulence factors and mechanisms underlying small leaf formation caused by jujube witches’ broom (JWB) phytoplasmas remain largely unknown. Here, effectors SJP1 and SJP2 from JWB phytoplasmas were identified to induce small leaf formation in jujube. In vivo interaction and expression assays showed that SJP1 and SJP2 interacted with and stabilized ZjTCP2. Over-expression of SJP1 and SJP2 in jujube induced ZjTCP2 accumulation. In addition, the abundance of miRNA319f-1 was significantly repressed in leaves of SJP1 and SJP2 transgenic jujube plants and showed the opposite pattern with its target ZjTCP2, which was consistent with that in the diseased leaves. Overexpression of ZjTCP2 in Arabidopsis promoted ectopic leaves arising from the adaxial side of cotyledons and reduced the leaf size. Constitutive expression of the miRNA319f-1 precursor in the 35S::ZjTCP2 background reduced the abundance of ZjTCP2 mRNA and reversed the cotyledon and leaf defects in Arabidopsis. Therefore, these observations suggest that effectors SJP1 and SJP2 induced small leaf formation, at least partly, by interacting with and activating ZjTCP2 expression both at the transcriptional and protein levels, providing new knowledge about small leaf formation caused by phytoplasmas in woody plants.

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First Report of Association of a ‘Candidatus Phytoplasma aurantifolia’ 16SrII-D Related Phytoplasma with Geranium (Pelargonium hortorum) Little Leaf Disease in Iran

Cited by 3 publications

References 6 publications

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces

Phytoplasma aurantifolia (lime witches' broom phytoplasma)

The ‘Candidatus Phytoplasma ziziphi’ effectors SJP1/2 negatively control leaf size by stabilizing the transcription factor ZjTCP2 in jujube

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