First report of Anaplasma ovis in pupal and adult Melophagus ovinus (sheep ked) collected in South Xinjiang, China

Zhao, Li; He, Bo; Li, Kairui; Li, Fēi; Zhang, Luyao; Li, Xianqiang; Liu, Yonghong

doi:10.1186/s13071-018-2788-6

Cited by 21 publications

(18 citation statements)

References 27 publications

(55 reference statements)

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“…In recent years, Melophagus ovinus has been frequently reported in Tibet [4], Xinjiang [2, 3, 5], and Gansu [6] in China. At least 13 pathogens have been reported in M .…”

Section: Discussionmentioning

confidence: 99%

“…The presence of M . ovinus has been reported in many countries in Africa, North America, Europe, Oceania, and Asia [2]. In China, M .…”

Section: Introductionmentioning

confidence: 99%

“…In particular, M . ovinus can carry and transmit multiple pathogens and has thus become a worldwide concern [2, 3]. China has reported at least 13 pathogens detected in M .…”

Section: Introductionmentioning

confidence: 99%

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First report of border disease virus in Melophagus ovinus (sheep ked) collected in Xinjiang, China

Liu¹,

He²,

Li³

et al. 2019

PLoS ONE

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Melophagus ovinus (sheep ked) is a blood-sucking ectoparasite that is parasitic primarily on sheep. It is widely distributed in different geographical regions worldwide. In China, it has been mainly found in Xinjiang, Gansu, and Tibet in recent years. In addition to causing direct damage to the animal hosts, M . ovinus also carries pathogens and serves as a vector for disease transmission. Border disease virus (BDV) is a positive-sense, single-stranded RNA pestivirus that mainly infects and causes border disease (BD) in sheep and goats worldwide. Since 2012, this disease has been reported in 4 provinces in China. In the present study, we investigated the presence of BDV in M . ovinus from Xinjiang and Gansu. Frozen M . ovinus collected during 2017 and 2018 from Xinjiang and Gansu and preserved in our laboratory were studied. First, total RNA of M . ovinus was extracted, followed by reverse transcription, PCR (RT-PCR) amplification of the 5′-UTR of BDV, and sequencing of the amplified products. Finally, the sequencing results were analyzed using DNAStar, MEGA 5.0 molecular biology software, and the BLAST online platform. The results from RT-PCR and sequencing analyses showed that among the samples included in the study, only the M . ovinus collected from Qinghe County in Alta, Xinjiang in 2018 tested positive for BDV. BLAST analysis showed that the viral strain with the most similar nucleotide identity to the sequence of the China/BDV/2018 fragment was the goat-derived BDV strain AH12-02 collected in Anhui, China, in 2012. A phylogenetic-tree analysis showed the strain to exhibit a BDV-3 genotype. This is the first report globally on BDV detected in M . ovinus and is also the first report of BDV discovered in Xinjiang, China. This study reconfirms the presence of BDV in China.

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“…In recent years, Melophagus ovinus has been frequently reported in Tibet [4], Xinjiang [2, 3, 5], and Gansu [6] in China. At least 13 pathogens have been reported in M .…”

Section: Discussionmentioning

confidence: 99%

“…The presence of M . ovinus has been reported in many countries in Africa, North America, Europe, Oceania, and Asia [2]. In China, M .…”

Section: Introductionmentioning

confidence: 99%

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First report of border disease virus in Melophagus ovinus (sheep ked) collected in Xinjiang, China

Liu¹,

He²,

Li³

et al. 2019

PLoS ONE

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“…A. ovis has been considered as the etiological agents of anaplasmosis of domestic ruminants and it has been widely detected in sheep, goat, cattle, wild deer and many tick species around the world (Battilani et al, 2017;Han et al, 2019). In previous reports, all sheep keds (100%, 81/81) were found to harbor A. ovis in Hungary (Hornok et al, 2011) and 28 specimens (including ve pupal specimens) (31.8%,28/88) collected in 2016 and 2017 in Xinjiang, China tested positive for A. ovis (Zhao et al, 2018). The prevalence (39.1%) and genetic characteristic (Genotype I) of A. ovis in M. ovinus in this study was concurred with other reports published in Xinjiang, but lower than Hungary.…”

Section: Discussionmentioning

confidence: 98%

“…Chen et al (2011) reported Borrelia burgdorferi sensu lato in sheep keds in Tibet, China. Recently, in China, Anaplasma ovis (Zhao et al, 2018), Rickettsia raoultii and R. slovaca (Liu et al, 2016), Theileria ovis (Zhao et al, 2019), and Border disease virus (BDV) (Liu et al, 2019) have also been detected in M. ovinus in Xinjiang Uygur Autonomous Region, northwestern China.…”

Section: Introductionmentioning

confidence: 99%

Vector-Borne Pathogens With Veterinary and Public Health Significance in Melophagus Ovinus (Sheep Ked) From the Qinghai-Tibet Plateau

Zhang

Wang

Liu

et al. 2020

Preprint

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BackgroundMelophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss and skin damage to the animal hosts, M. ovinus also serves as a vector for a variety of pathogens and is highly likely to participate in the life and transmission cycle of pathogenic organisms. MethodsHerein, we investigated the presence and molecular characterization of vector-borne pathogens in M. ovinus from Qinghai-Tibet Plateau, China. ResultsA total of 92 M. ovinus pools (n=276) collected from Qinghai province of China were screened for the presence of selected vector-borne pathogens. The overall prevalence of A. ovis, A. bovis, A. phagocytophilum, and T. ovis in M. ovinus was 39.1%, 17.4%, 9.8%, and 89.1%, respectively. All of the samples were negative for BDV, other Anaplasma species, Babesia spp., Rickettsia spp., and Borrelia spp. Co-infection of different Anaplasma species and T. ovis occurred in 51.2% of all samples with T. ovis. The positive rates of A. ovis, A. bovis, and A. phagocytophilum in different region and altitude of the sampling sites were significantly different. Sequence and phylogenetic analysis of target genes confirmed their identity with corresponding pathogens. ConclusionOur results elucidate the occurrence and genetic diversity of Anaplasma spp. and Theileria spp. in M. ovinus, which could act as potential zoonotic reservoirs. To the best of our knowledge, this is the first report of the detection of A. bovis and A. phagocytophilum DNA in M. ovinus. This study gives the first extensive molecular survey of vector-borne pathogens with veterinary and public health significance in M. ovinus from the Qinghai-Tibet Plateau, China.

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Keds and Bat Flies (Hippoboscidae, Nycteribiidae and Streblidae)

Andreani

Sacchetti

Belcari

2022

Encyclopedia of Infection and Immunity

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First report of Anaplasma ovis in pupal and adult Melophagus ovinus (sheep ked) collected in South Xinjiang, China

Cited by 21 publications

References 27 publications

First report of border disease virus in Melophagus ovinus (sheep ked) collected in Xinjiang, China

First report of border disease virus in Melophagus ovinus (sheep ked) collected in Xinjiang, China

Vector-Borne Pathogens With Veterinary and Public Health Significance in Melophagus Ovinus (Sheep Ked) From the Qinghai-Tibet Plateau

Keds and Bat Flies (Hippoboscidae, Nycteribiidae and Streblidae)

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