First report of an astrovirus type 5 gastroenteritis outbreak in a residential elderly care home identified by sequencing

Jarchow-MacDonald, Anna; Halley, Shona; Chandler, Daniel; Gunson, Rory; Shepherd, Samantha J.; Parcell, Benjamin J.

doi:10.1016/j.jcv.2015.11.006

Cited by 24 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also other studies have shown that the role of porcine astrovirus in the pathogenesis of pig diarrhea is not completely clear 28 . In contrast, associations between diarrhea and human astrovirus infections have been made 29 . A recent study in 5 European countries (Hungary, Spain, Germany, Austria and Sweden) have indicated the widespread of porcine astroviruses in the swine population.…”

Section: Discussionmentioning

confidence: 99%

Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus

Theuns

Vanmechelen

Bernaert

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Enteric diseases in swine are often caused by different pathogens and thus metagenomics are a useful tool for diagnostics. The capacities of nanopore sequencing for viral diagnostics were investigated here. First, cell culture-grown porcine epidemic diarrhea virus and rotavirus A were pooled and sequenced on a MinION. Reads were already detected at 7 seconds after start of sequencing, resulting in high sequencing depths (19.2 to 103.5X) after 3 h. Next, diarrheic feces of a one-week-old piglet was analyzed. Almost all reads (99%) belonged to bacteriophages, which may have reshaped the piglet’s microbiome. Contigs matched Bacteroides, Escherichia and Enterococcus phages. Moreover, porcine kobuvirus was discovered in the feces for the first time in Belgium. Suckling piglets shed kobuvirus from one week of age, but an association between peak of viral shedding (106.42–107.01 copies/swab) and diarrheic signs was not observed during a follow-up study. Retrospective analysis showed the widespread (n = 25, 56.8% positive) of genetically moderately related kobuviruses among Belgian diarrheic piglets. MinION enables rapid detection of enteric viruses. Such new methodologies will change diagnostics, but more extensive validations should be conducted. The true enteric pathogenicity of porcine kobuvirus should be questioned, while its subclinical importance cannot be excluded.

show abstract

Section: Discussionmentioning

confidence: 99%

Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus

Theuns

Vanmechelen

Bernaert

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Astrovirus is responsible for a considerable proportion of gastroenteritis cases in young children, the elderly, and the immunosuppressed (75)(76)(77). NoV GII, which is considered to be an etiologic agent frequently associated with cases of gastroenteritis, was found in only 5% of water samples, perhaps reflecting the agricultural impact in the watershed (as reflected also in the somewhat higher level of detection of NoV GIII, at ϳ11%).…”

Section: Detection and Densities Of F-specific Coliphage And Viruses mentioning

confidence: 99%

Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

Jones

Brassard

Topp

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

From the years 2008 to 2014, a total of 1,155 water samples were collected (spring to fall) from 24 surface water sampling sites located in a mixed-used but predominantly agricultural (i.e., dairy livestock production) river basin in eastern Ontario, Canada. Water was analyzed for viable F-specific DNA (F-DNA) and F-specific RNA (F-RNA) (genogroup I [GI] to GIV) coliphage and a suite of molecularly detected viruses (norovirus [GI to GIV], torque teno virus [TTV], rotavirus, kobuvirus, adenovirus, astrovirus, hepatitis A, and hepatitis E). F-DNA and F-RNA coliphage were detected in 33 and 28% of the samples at maximum concentrations of 2,000 and 16,300 PFU · 100 ml Ϫ1 , respectively. Animal TTV, human TTV, kobuvirus, astrovirus, and norovirus GIII were the most prevalent viruses, found in 23, 20, 13, 12, and 11% of samples, respectively. Viable F-DNA coliphage was found to be a modest positive indicator of molecularly detected TTV. F-RNA coliphage, unlike F-DNA coliphage, was a modest positive predictor of norovirus and rotavirus. There were, however, a number of significant negative associations among F-specific coliphage and viruses. F-DNA coliphage densities of Ͼ142 PFU · 100 ml Ϫ1 delineated conditions when ϳ95% of water samples contained some type of virus. Kobuvirus was the virus most strongly related to detection of any other virus. Land use had some associations with virus/F-specific coliphage detection, but season and surface water flow were the variables that were most important for broadly delineating detection. Higher relative levels of detection of human viruses and human F-RNA coliphage were associated with higher relative degrees of upstream human land development in a catchment.IMPORTANCE This study is one of the first, to our knowledge, to evaluate relationships among F-specific coliphages and a large suite of enteric viruses in mixed-use but agriculturally dominated surface waters in Canada. This study suggested that relationships between viable F-specific coliphages and molecularly detected viruses do exist, but they are not always positive. Caution should be employed if viable F-specific coliphages are to be used as indicators of virus presence in surface waters. This study elucidates relative effects of agriculture, wildlife, and human activity on virus and F-specific coliphage detection. Seasonal and meteorological attributes play a strong role in the detection of most virus and F-specific coliphage targets.KEYWORDS viruses, F-specific coliphages, pollution, indicators, surface water, microbial source tracking

show abstract

“…Em pesquisas sorológicas no Reino Unido e nos Estados Unidos tem mostrado que até 70% das crianças até 5 anos de idades já foram infectadas pelo vírus (BOSCH et al, 2014). Na Escócia, em um asilo foi reportado um surto de gastrenterite que teve o AstV tipo 5 como agente etiológico (JARCHOW-MACDONALD et al, 2015). 24 amostras foram positivas de HAdV, ou seja, 3,5% de prevalência (MOYO et al, 2014).…”

Section: Astrovírusunclassified

Viroses Entéricas: Principais Patologias De Veiculação Hídrica E Suas Manifestações Clínicas

Oliveira

Röhnelt²,

Ritzel³

et al. 2020

RCO

View full text Add to dashboard Cite

Vírus entéricos podem causar diversas infecções que acometem os seres humanos e são disseminados pelos meios hídricos, sendo o esgoto doméstico um dos principais contaminantes. A relação ambiente-saúde é de extrema importância uma vez que tais microrganismos podem permanecer viáveis no ambiente e serem disseminados em meio hídrico devido à falta de saneamento básico. O objetivo deste artigo foi abordar temas relacionados aos vírus entéricos, tais como Enterovírus (EV), Rotavírus (RV), Astrovírus (AstV), Mastadenovirus (AdVH) e Norovírus (NoV), elencando as principais doenças que acometem ao homem, transmitidas através da água sem tratamento adequado. Este estudo é um artigo de revisão que utilizou plataformas como Scielo, Pubmed, Medline e Science Direct para pesquisas de diversos trabalhos relacionados. É imprescindível o conhecimento científico sobre a relação hídrica/ambiente com a saúde humana, uma vez que os vírus são responsáveis por diversas enfermidades e atingem a população a qual carece de uma melhor qualidade hídrica.Palavras-chave: Gastroenterites. Vírus Entéricos. Água.ABSTRACTEnteric viruses can cause various infections affecting humans and are spread by water, being the domestic sewage a major contaminants. The environment-health relationship is of extreme importance since such micro-organisms can remain viable in the environment and be disseminated in water because of lack of basic sanitation. The objective of this article was to address issues related to enteric viruses, such asEnterovirus (EV), Rotavirus (RV), Astrovirus (AstV), Mastadenovirus (AdVH) e Norovirus (NoV), by listing their major diseases that affect humans, transmitted through water without adequate treatment. This study is a review article that used platforms such as Scielo, Pubmed, Medline and Science Direct for research on several related works. Scientific knowledge about the relationship between water and the environment with human health is essential, since viruses are responsible for several diseases and reach the population which lacks a better water quality.Keywords: Gastroenteritis. Enteric Viruses. Water.

show abstract

First report of an astrovirus type 5 gastroenteritis outbreak in a residential elderly care home identified by sequencing

Cited by 24 publications

References 20 publications

Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus

Nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus

Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

Viroses Entéricas: Principais Patologias De Veiculação Hídrica E Suas Manifestações Clínicas

Contact Info

Product

Resources

About