First Report of Alfalfa Leaf Curl Virus from Alfalfa in Iran

Davoodi, Zohreh; Heydarnejad, Jahangir; Massumi, Hossain; Richet, Cécile; Galzi, Serge; Filloux, Denis

doi:10.1094/pdis-04-18-0571-pdn

Cited by 5 publications

(4 citation statements)

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“…In addition, this study suggested that ALCV was probably widely distributed across the Mediterranean basin [ 11 ]. Consistent with this hypothesis, ALCV was subsequently reported in 2018 from Jordan, Syria, Lebanon, and Tunisia [ 12 ] as well as from the non-Mediterranean countries Iran [ 13 ] and Argentina [ 14 ].…”

Section: Introductionmentioning

confidence: 66%

“…All samples from Namibia and South Africa tested negative for ALCV. Overall, 85 full length genome sequences were reported in this study (GenBank accession numbers: MG792020–MG792053; MH603810–MH603860), which were analyzed together with the 35 complete genome sequences recovered in previous studies [ 7 , 11 , 12 , 13 , 14 ] ( Supplementary Materials Table S1 ). The sizes of these 120 complete ALCV genome sequences ranged from 2712 nt to 2769 nt, and they all shared >80.2% genome-wide pairwise identity.…”

Section: Resultsmentioning

confidence: 99%

“…PCR-based detection of ALCV from alfalfa plants collected in all countries other than Argentina and Iran was performed using the primer pair ALCV-187F (5′-TGG AAT ATT GTG CTG CTT GG-3′) and ALCV-971R (5′-ATT TTG GGA CTT GTG CTC CA-3′), as previously described in Bernardo et al (2016) [ 11 ]. The presence of ALCV from the Iranian alfalfa samples was tested using PCR with the primer pair Gemini F1 (5′-ATG ATG GAT AAT TCA AAC CC-3′) and Gemini R2 (5′-CAC CTC CAC TGT CTT GTC CA-3′), as described in Davoodi et al (2018) [ 13 ]. The presence of ALCV from the Argentinean alfalfa samples was tested using PCR with the KAPA HiFi HotStart PCR Kit (Kapa Biosystems, Wilmington, MA, USA) following the manufacturer’s protocol with the primer pair ALCV CPF (5′- GAG AAC GTA TGG ATT GGT C-3′) and ALCV CPR (5′-AGT GTA TGC GTT CTT CTG G-3′).…”

Section: Methodsmentioning

confidence: 99%

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The Westward Journey of Alfalfa Leaf Curl Virus

Davoodi

Bejerman

Richet

et al. 2018

Viruses

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Alfalfa leaf curl virus (ALCV), which causes severe disease symptoms in alfalfa (Medicago sativa L.) and is transmitted by the widespread aphid species, Aphis craccivora Koch, has been found throughout the Mediterranean basin as well as in Iran and Argentina. Here we reconstruct the evolutionary history of ALCV and attempt to determine whether the recent discovery and widespread detection of ALCV is attributable either to past diagnostic biases or to the emergence and global spread of the virus over the past few years. One hundred and twenty ALCV complete genome sequences recovered from ten countries were analyzed and four ALCV genotypes (ALCV-A, ALCV-B, ALCV-C, and ALCV-D) were clearly distinguished. We further confirm that ALCV isolates are highly recombinogenic and that recombination has been a major determinant in the origins of the various genotypes. Collectively, the sequence data support the hypothesis that, of all the analyzed locations, ALCV likely emerged and diversified in the Middle East before spreading to the western Mediterranean basin and Argentina.

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Section: Introductionmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Westward Journey of Alfalfa Leaf Curl Virus

Davoodi

Bejerman

Richet

et al. 2018

Viruses

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“…Containing abundant protein and micronutrients, alfalfa also becomes the natural reservoir of a large variety of viruses. To date, more than 50 viral species have been identified, with some having significant negative impacts on quality, persistence, and production, with symptoms such as dwarfism, yellowing, leaf curling, and mottling, which affect the photosynthetic efficiency [ 6 , 7 , 8 ]. Virus pathogens have been shown to have a global impact on alfalfa yield and quality [ 6 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence, Distribution, and Transmission of Alfalfa Viruses in China

Shang

Liu

et al. 2022

Viruses

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Alfalfa (Medicago sativa L.) is one of the most important quality forages worldwide and is cultivated throughout China. Alfalfa is susceptible to a variety of viral diseases during its growth, which has caused huge amounts of commercial losses. However, the profile of the alfalfa virus in China remains ambiguous and the viruses transmitted by Odontothrips loti (Haliday), dominant insect pests in alfalfa, are also poorly understood. In the present study, virus diversity was investigated in the primary alfalfa-growing areas in China. A total of 18 alfalfa viruses were identified through RNA-sequencing (RNA-seq) and reverse transcription-polymerase chain reaction (RT-PCR). Two new plant viruses, Medicago sativa virus 1 (MsV1) and Medicago sativa luteovirus 1 (MsLV1), were detected for the first time. Another four viruses, including the Alfalfa ringspot-associated virus (ARaV), Alfalfa virus F (AVF), Alfalfa enamovirus 1 (AEV1), and Alfalfa deltaparitivirus (ADPV), were reported in China for the first time as well. Both Alfalfa mosaic virus (AMV) and Medicago sativa alphapartitivirus 2 (MsAPV2) are the dominant pathogens, with an infection incidence of 91.7–100%, and 74.4–97.2%, respectively. Additionally, O. loti with first- and second-instar nymphs were shown to acquire the AMV within 0.25 h of feeding on a virus-infected alfalfa. Transmission by thrips to healthy alfalfa plants was also demonstrated. Additionally, we clarified the dynamic changes in the AMV in pre-adult stages of O. loti, which indicated that the AMV is propagated in the nymph stage of O. loti. These findings provide valuable information for understanding the alfalfa virome, confirm the role thrips O. loti plays in alfalfa virus transmission, and improve our fundamental knowledge and management of diseases in China.

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Alfalfa leaf curl virus

2022

CABI Compendium

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First Report of Alfalfa Leaf Curl Virus from Alfalfa in Iran

Cited by 5 publications

References 4 publications

The Westward Journey of Alfalfa Leaf Curl Virus

The Westward Journey of Alfalfa Leaf Curl Virus

Occurrence, Distribution, and Transmission of Alfalfa Viruses in China

Alfalfa leaf curl virus

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