First report of a putative involvement of the NMDA pathway in Pacific oyster (Crassostrea gigas) development: Effect of NMDA receptor ligands on oyster metamorphosis with implications for bivalve hatchery management

Vogeler, Susanne; Miller-Ezzy, Penny; Li, Xiaoxu; Wikfors, Gary H.; Joyce, Alyssa

doi:10.1016/j.aquaculture.2018.07.048

Cited by 13 publications

(8 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All metamorphosis assays were conducted following a general protocol as previously described (2)(3)(4). In brief, competent pediveliger larvae (shell length 300-330 µm, visible eyespot, crawling behaviour) were placed in FSW in glass shell vials, continuously exposed to chemical compounds for either 1 h, 3 h, 6 h or 24 h; the chemicals were then removed by pipetting, and 10 ml FSW including microalgae was added to each vial.…”

Section: Animal Husbandry and Metamorphosis Assaymentioning

confidence: 99%

“…Complex neuroendocrine pathways and neurotransmitters have been proposed as regulators for metamorphosis in bivalves (reviewed in (1)), but there are many unknown factors regulating this process. Recently, we provided evidence for a previously unexplored regulatory pathway involved in bivalve metamorphosis involving the transmembrane N-Methyl-D-aspartate (NMDA) receptor (2)(3)(4). As ligand-gated and voltage-dependent ion channels, NMDA receptors are located in synaptic cell membranes and are activated by agonists, predominantly glutamate, co-agonists (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bivalves are NO Different: Nitric Oxide as Negative Regulator of Metamorphosis in the Pacific Oyster, Crassostrea Gigas.

Vogeler

Carboni

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background Nitric oxide (NO) is a presumed regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish.Results In this paper, we investigate the effects of NO pathway inhibitors and NO donors on metamorphosis induction in larvae of the Pacific oyster, Crassostrea gigas, demonstrating that the nitric oxides synthase (NOS) inhibitors s-methylisothiourea hemisulfate salt (SMIS), aminoguanidine hemisulfate salt (AGH) and 7-nitroindazole (7-NI) induce metamorphosis in a concentration-dependent manner ranging from 75%, 76–83% respectively. Additional induction resulted from exposures to 1H-[1, 2, 4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ), an inhibitor of soluble guanylyl cyclase, with which NO interacts to catalyse the synthesis of cyclic guanosine monophosphate (cGMP). Conversely, high concentrations of the NO donor sodium nitroprusside dihydrate in combination with metamorphosis inducers epinephrine, MK-801 or SMIS, significantly decreased metamorphosis. Expression of CgNOS also decreased in larvae after metamorphosis regardless of the inducers used, but intensified again post-metamorphosis in spat. Fluorescent detection of NO in competent larvae with DAF-FM diacetate and localisation of the oyster nitric oxide synthase CgNOS expression by in-situ hybridisation showed that NO occurs primarily in two key larval structures, the velum and foot. cGMP was also detected in the foot using immunofluorescent assays, and is potentially involved in the foot’s smooth muscle relaxation.Conclusion Together, these results suggest that NO is a negative regulator of metamorphosis in Pacific oyster larvae, and that NO reduction induces metamorphosis by inhibiting swimming or crawling behaviour, in conjunction with a cascade of additional neuroendocrine downstream responses.

show abstract

Section: Animal Husbandry and Metamorphosis Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bivalves are NO Different: Nitric Oxide as Negative Regulator of Metamorphosis in the Pacific Oyster, Crassostrea Gigas.

Vogeler

Carboni

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The catecholamine epinephrine hydrochloride (EPI), the NOS inhibitors S-methylisothiourea hemisulfate salt (SMIS) and aminoguanidine hemisulfate salt (AGH) as well as the NO donor sodium nitroprusside dihydrate (SNP) were purchased from Sigma-Aldrich. The irreversible inhibitor of soluble guanylyl cyclase 1H- [1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ) and the NO donor 3-morpholinosydnonimine chloride (SIN-1) were obtained from AdipoGen Life Sciences and the NOS inhibitors L-NG-nitroarginine methyl ester (L-NAME) hydrochloride, L-NG-nitroarginine (L-NNA) and 7-nitroindazole (7-NI) were purchased from Cayman chemicals. Additional chemicals were Levodopa (L-DOPA), obtained from Santa Cruz Biotechnology, as well as (+)-MK 801 maleate (MK-801) and Ifenprodil (+)-tartrate salt (ifenprodil), both obtained from Selleckchem.…”

Section: Chemical Reagentsmentioning

confidence: 99%

“…All metamorphosis assays were conducted following a general protocol as previously described (3)(4)(5). In brief, competent pediveliger larvae (shell length 300-330 µm, visible eyespot, crawling behaviour) were placed in FSW in glass shell vials using a pipet with a large tip opening, continuously exposed to chemical compounds for either 1 h, 3 h, 6 h or 24 h; the chemicals were then removed by pipetting, and 10 ml FSW including microalgae was added to each vial.…”

Section: Animal Husbandry and Metamorphosis Assaymentioning

confidence: 99%

“…Complex neuroendocrine pathways and neurotransmitters have been proposed as regulators for metamorphosis in bivalves (reviewed in (2)), but there are many unknown factors regulating this process. Recently, we provided evidence for a previously unexplored regulatory pathway involved in bivalve metamorphosis involving the transmembrane N-Methyl-D-aspartate (NMDA) receptor (3)(4)(5). As ligand-gated and voltage-dependent ion channels, NMDA receptors are located in synaptic cell membranes and are activated by agonists, predominantly glutamate, co-agonists (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Vogeler

Carboni

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Nitric oxide (NO) is presumed to be a regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish.Results: In this paper, we investigate the effects of NO pathway inhibitors and NO donors on metamorphosis induction in larvae of the Pacific oyster, Crassostrea gigas. The nitric oxides synthase (NOS) inhibitors s-methylisothiourea hemisulfate salt (SMIS), aminoguanidine hemisulfate salt (AGH) and 7-nitroindazole (7-NI) induced metamorphosis ranging from 75%, 76% to 83% respectively, and operating in a concentration-dependent manner. Additional induction of up to 54% resulted from exposures to 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ), an inhibitor of soluble guanylyl cyclase, with which NO interacts to catalyse the synthesis of cyclic guanosine monophosphate (cGMP). Conversely, high concentrations of the NO donor sodium nitroprusside dihydrate in combination with metamorphosis inducers epinephrine, MK-801 or SMIS, significantly decreased metamorphosis, although a potential harmful effect of excessive NO unrelated to metamorphosis pathway cannot be excluded. Expression of CgNOS also decreased in larvae after metamorphosis regardless of the inducers used, but intensified again post-metamorphosis in spat. Fluorescent detection of NO in competent larvae with DAF-FM diacetate and localisation of the oyster nitric oxide synthase CgNOS expression by in-situ hybridisation showed that NO occurs primarily in two key larval structures, the velum and foot. cGMP was also detected in the foot using immunofluorescent assays, and is potentially involved in the foot’s smooth muscle relaxation.Conclusion: Together, these results suggest that the NO pathway acts as negative regulator of metamorphosis in Pacific oyster larvae, and that NO reduction induces metamorphosis by inhibiting swimming or crawling behaviour, in conjunction with a cascade of additional neuroendocrine downstream responses.

show abstract

Transcriptomic analysis of differentially expressed genes in the larval settlement and metamorphosis of peanut worm Sipunculus nudus

Cao

Zhong

Yang

et al. 2020

Aquaculture Reports

View full text Add to dashboard Cite

First report of a putative involvement of the NMDA pathway in Pacific oyster (Crassostrea gigas) development: Effect of NMDA receptor ligands on oyster metamorphosis with implications for bivalve hatchery management

Cited by 13 publications

References 57 publications

Bivalves are NO Different: Nitric Oxide as Negative Regulator of Metamorphosis in the Pacific Oyster, Crassostrea Gigas.

Bivalves are NO Different: Nitric Oxide as Negative Regulator of Metamorphosis in the Pacific Oyster, Crassostrea Gigas.

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Transcriptomic analysis of differentially expressed genes in the larval settlement and metamorphosis of peanut worm Sipunculus nudus

Contact Info

Product

Resources

About