First Report of a Hip Prosthetic and Joint Infection Caused by Lactococcus garvieae in a Woman Fishmonger

Aubin, Guillaume Ghislain; Bémer, Pascale; Guillouzouic, A.; Crémet, Lise; Touchais, S.; Fraquet, N.; Boutoille, David; Reynaud, Alain; Lepelletier, Didier; Corvec, Stéphane

doi:10.1128/jcm.00065-11

Cited by 43 publications

(33 citation statements)

References 18 publications

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“…16S rRNA gene amplification and sequencing were performed with universal primers 27f and 1492r as previously described yielding a 1424 bp fragment (Aubin et al, 2011). According to the BIBI database (https://umr5558-bibiserv.…”

Section: Strains Nts 31307302mentioning

confidence: 99%

Propionibacterium namnetense sp. nov., isolated from a human bone infection

Aubin

Bémer

Kambarev

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus Propionibacterium, most closely related to Propionibacterium acnes subsp. acnes and Propionibacterium acnes subsp. elongatum with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for rpoB, recA and gyrB clearly separated the novel organism from all species and subspecies of the genus Propionibacterium. However, a DNA-DNA hybridization analysis between the novel organism and the type strain P. acnes ATCC 6919T revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cut-off value of 95 % for species delineation. The major fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 17 : 0 . The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of P. acnes On the basis of the results presented, the organism represents a novel member of the genus Propionibacterium for which the name Propionibacterium namnetense sp. nov. is proposed. The type strain is NTS 31307302Pathogenesis of bone and joint infections (BJI) is explained by the growth of microorganisms in biofilms and microcolonies, making these infections difficult to diagnose, eradicate and treat (Zimmerli et al., 2004). Staphylococci are involved in 50 to 60 % of BJI cases (Corvec et al., 2012), and members of the genus Propionibacterium are also †These authors contributed equally to this work Abbreviation: MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight.

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Section: Strains Nts 31307302mentioning

confidence: 99%

Propionibacterium namnetense sp. nov., isolated from a human bone infection

Aubin

Bémer

Kambarev

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…In addition, skin wounds are suspected to be the portal of entry for asymptomatic bacteremia caused by L. garvieae, as in the case which occurred in an immune-suppressed woman fishmonger who lived near the sea (26). Likewise, our patient was a fisherman and frequently ingested raw fish or shellfish before this presentation.…”

Section: Case Reportmentioning

confidence: 78%

First Report of Human Acute Acalculous Cholecystitis Caused by the Fish Pathogen Lactococcus garvieae

Kim

Cho

et al. 2013

J Clin Microbiol

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We report herein the first case of acute acalculous cholecystitis caused by Lactococcus garvieae, which is known as a fish pathogen. A 69-year-old fisherman underwent laparoscopic cholecystectomy due to severe inflammation in the gallbladder. The isolate obtained from the gallbladder was identified as L. garvieae by 16S rRNA and manganese-dependent superoxide dismutase (sodA) gene sequence analysis. CASE REPORTA 69-year-old man presented at Inje University Ilsan Paik Hospital (Goyang, Republic of Korea) with severe upper abdominal and postprandial pains for 2 days. His medical history included gastric ulcer perforation, fatty liver, and chronic alcoholism with tobacco use. The gastric ulcer perforation had been treated with exploratory laparotomy 19 years ago; however, the patient took no prescribed medications for fatty liver and alcoholism during that time. The patient was a fisherman by occupation, and during the middle period of his life worked at a fish culture farm located at the Han-tan and Nam-han rivers in the Republic of Korea. He occasionally ingested raw rainbow trout (Oncorhynchus mykiss) at that time and continued eating raw freshwater fish or shellfish frequently before this presentation. He had never traveled overseas.Physical examination on admission showed crouching with a blood pressure of 130/80 mm Hg, a heart rate of 73 beats/min, a respiratory rate of 20/min, and a body temperature of 36.3°C. The patient exhibited no evidence of murmur, jugular vein engorgement, or liver cirrhosis. His abdomen was mildly distended and soft, with normoactive bowel sounds, tenderness in the right upper quadrant, positive Murphy's sign, and no rebound tenderness. The results of routine tests were as follows: white cell count, 17,000 cells/l; polymorphoneutrophil count, 14,340 cells/l; hemoglobin, 15.1 g/dl; erythrocyte sedimentation rate (ESR), 15 mm/h; electrolytes (Na, K, and Cl), 138, 4.8, and 104 mEq, respectively; blood urea nitrogen (BUN) and creatinine, 15 and 1.1 mg/dl, respectively; aspartate transferase (AST) and alanine transaminase (ALT), 94 and 73 IU/liter, respectively; total bilirubin, 2.6 mg/dl; and urine analysis, clear.Abdominopelvic computed tomography (CT) findings were as follows: distension of the gallbladder, edematous wall thickening, and mild hyperemic change at the gallbladder bed. In addition, attenuation of parenchyma was lower in the liver than in the spleen. There were no remarkable findings in the pancreas, kidneys, spleen, or urinary bladder. No evidence of enlarged lymph nodes or ascites was found on CT scans. Therefore, the patient underwent laparoscopic cholecystectomy with closed drainage (Baro-vac), which showed a thickened, distended, hyperemic and edematous gallbladder. Obstructive stones were not observed in the gallbladder. There were no specific abnormalities in other organs, except that the liver angle was blunt. After the operation, the patient was treated with cefminox sodium (Meicelin) (2 g twice a day [b.i. Three specimens (one specimen each from the patient's bil...

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“…Según el tipo de infección, hubo 20 endocarditis infecciosas, (11 de válvulas mitrales nativas [4][5][6][7][8][9][10][11][12] , tres de prótesis de válvula mitral 7,13,14 , tres válvulas aórticas nativas 2,12,15 , dos prótesis de válvula aórtica 9,16 y una válvula tricúspidea 17 ), dos casos de bacteriemia 4,18 , dos casos de septicemia 4,19 y dos casos de peritonitis 4,20 . Otros fueron casos aislados de absceso hepático 21 , osteomielitis 22 , colecistitis aguda 23 , espondilitis 24 , infección de prótesis de cadera 25 y un hematoma subdural 9 . La mayoría de los casos se encuentran entre los 40 y 85 años de edad con un solo caso pediátrico, de un paciente de 10 años.…”

Section: Discussionunclassified