First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens

Cárdenas, Paco; Moore, Jon A.

doi:10.1007/s12526-017-0775-3

Cited by 18 publications

(12 citation statements)

References 53 publications

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“…Due to the formalin fixation, we were not able to extract DNA for molecular analysis, and any attempts to barcode the mitochondrial COI gene, including the mini-barcode protocol used in other tetractinellids (Cárdenas & Moore, 2017) were unsuccessful.…”

Section: Methodsmentioning

confidence: 99%

Rock sponges (lithistid Demospongiae) of the Northeast Atlantic seamounts, with description of ten new species

Carvalho

Cárdenas

Ríos

et al. 2020

PeerJ

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Background Lithistid demosponges, also known as rock sponges, are a polyphyletic group of sponges which are widely distributed. In the Northeast Atlantic (NEA), 17 species are known and the current knowledge on their distribution is mainly restricted to the Macaronesian islands. In the Mediterranean Sea, 14 species are recorded and generally found in marine caves. Methods Lithistids were sampled in nine NEA seamounts during the scientific expeditions Seamount 1 (1987) and Seamount 2 (1993) organized by the MNHN of Paris. Collected specimens were identified through the analyses of external and internal morphological characters using light and scanning electron microscopy, and compared with material from various museum collections as well as literature records. Results A total of 68 specimens were analysed and attributed to 17 species across two orders, seven families, and seven genera, representing new records of distribution. Ten of these species are new to science, viz. Neoschrammeniella inaequalis sp. nov., N. piserai sp. nov., N. pomponiae sp. nov., Discodermia arbor sp. nov., D. kellyae sp. nov., Macandrewia schusterae sp. nov., M. minima sp. nov., Exsuperantia levii sp. nov., Leiodermatium tuba sp. nov. and Siphonidium elongatus sp. nov., and are here described and illustrated. New bathymetric records were also found for D. ramifera, D. verrucosa and M. robusta. The Meteor seamount group has a higher species richness (15 species) compared to the Lusitanian seamount group (six species). The majority of the species had their distribution restricted to one seamount, and ten are only known from a single locality, but this can be a result of sample bias. Discussion The number of species shared between the seamounts and the Macaronesian islands is very reduced. The same pattern repeats between the NEA and Mediterranean Sea. This study demonstrates that NEA seamounts are ecosystems with a higher diversity of lithistids than previously thought, increasing the number of lithistids known to occur in the NEA and Mediterranean Sea from 26 to 36 species.

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Section: Methodsmentioning

confidence: 99%

Rock sponges (lithistid Demospongiae) of the Northeast Atlantic seamounts, with description of ten new species

Carvalho

Cárdenas

Ríos

et al. 2020

PeerJ

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“…Short DNA markers, so-called minimalist DNA barcodes (Hajibabaei et al 2006), can facilitate genotype comparison with degenerated type material using the Sanger sequencing methods if other methods for full-length amplification fail. Such minimalist DNA regions, which are sufficiently short for amplification from fragmented DNA templates but sufficiently variable for taxonomic distinction, are sequenced and analyzed (Cárdenas and Moore 2017).…”

Section: Introductionmentioning

confidence: 99%

Minimalist barcodes for sponges: a case study classifying African freshwater Spongillida

Erpenbeck

Steiner

Schuster

et al. 2019

Genome

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African sponges, particularly freshwater sponges, are understudied relative to demosponges in most other geographical regions. Freshwater sponges (Spongillida) likely share a common ancestor; however, their evolutionary history, particularly during their radiation into endemic and allegedly cosmopolitan groups, is unclear. Freshwater sponges of at least 58 species of 17 genera and four families are described from Central and Eastern Africa, but the diversity is underestimated due to limited distinguishable morphological features. The discovery of additional cryptic species is very likely with the use of molecular techniques such as DNA barcoding. The Royal Museum of Central Africa (MRAC, Tervuren, Belgium) hosts one of the largest collections of (Central) African freshwater sponge type material. Type specimens in theory constitute ideal targets for molecular taxonomy; however, the success is frequently hampered by DNA degradation and deamination, which are a consequence of suboptimal preservation techniques. Therefore, we genotyped African demosponge holotype material of the MRAC with specific short primers suitable for degenerated tissue and compare the results with the current, morphology-based classification. Our results demonstrate the utility of minimalistic barcodes for identification of sponges, potentially enabling efficient identification of individuals in taxonomic or metabarcoding studies, and highlight inconsistencies in the current freshwater sponge classification.

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“…For one specimen from Cape Verde (RMNH 3810) collected in 1982, the DNA was fairly degraded so we decided to amplify COI in two parts. For the first part we amplified the universal mini-barcode (130 bp) using primer pair LCO/Tetract-minibarR1 following the protocol from Cárdenas & Moore (2018). For the rest of the Folmer fragment we designed a forward primer in the mini-barcode to target Erylus species-ErylusCOI-F2 (5'-CTCCYGGATCAATGTTGGG-3')-and coupled it with HCO2198 always using the same COI PCR program.…”

Section: Introductionmentioning

confidence: 99%

From marine caves to the deep sea, a new look at Caminella (Demospongiae, Geodiidae) in the Atlanto-Mediterranean region

Cárdenas

Vacelet

Chevaldonné

et al. 2018

Zootaxa

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754

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Caminella Lendenfeld, 1894 is a poorly known Geodiidae genus with unclear phylogenetic relationships. In order to find new lines of evidence that could shed light on the evolutionary history of Caminella, we decided to revise type material and museum material, as well as examine new material from underwater caves and deep-sea ecosystems. In doing so, we formally show that Isops maculosus Vosmaer, 1894 and Caminella loricata Lendenfeld, 1894 are junior synonyms of Caminella intuta (Topsent, 1892). We discuss different spicule morphological phenotypes in C. intuta, which may be linked to silica availability. We also discovered two new species of deep-sea Caminella: 1) from Cape Verde (Caminella caboverdensis sp. nov.) and 2) from seamounts located south of the Azores archipelago and the North of Spain (Caminella pustula sp. nov.). We reveal that Caminella sterrasters have complex surface microstructures, unique amongst the Geodiidae, where actin tips are linked to each other. Molecular markers (COI, 28S (C1-D2) and 18S) sequenced for some specimens led to new phylogenetic analyses, which continue to suggest a close relationship of Caminella with the Erylinae and Calthropella; these affinities are discussed in light of morphological characters.

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First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens

Cited by 18 publications

References 53 publications

Rock sponges (lithistid Demospongiae) of the Northeast Atlantic seamounts, with description of ten new species

Rock sponges (lithistid Demospongiae) of the Northeast Atlantic seamounts, with description of ten new species

Minimalist barcodes for sponges: a case study classifying African freshwater Spongillida

From marine caves to the deep sea, a new look at Caminella (Demospongiae, Geodiidae) in the Atlanto-Mediterranean region

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