First record of the semi-slug Omalonyx unguis (d’Orbigny, 1837) (Gastropoda, Succineidae) in the Misiones Province, Argentina

Abstract

Omalonyx unguis (d’Orbigny, 1837) is a semi-slug inhabiting the Paraná river basin. This species belongs to Succineidae, a family comprising a few representatives in South America. In this work, we provide the first record for the species from Misiones Province, Argentina. Previous records available for Omalonyx in Misiones were identified to the genus level. We examined morphological characteristics of the reproductive system and used DNA sequences from cytochrome oxidase subunit I (COI) gene for species-specific identification. These new distributional data contribute to consolidate the knowledge of the molluscan fauna in northeastern Argentina.

The mitochondrial genome of the semi-slug Omalonyx unguis (Gastropoda: Succineidae) and the phylogenetic relationships within Stylommatophora

“…The individuals were relaxed in water with menthol crystals for 6 h, preserved in ethanol 96%, and deposited in the malacological collection at the Instituto de Biología Subtropical, CONICET–UNaM, Misiones Province, Argentina (IBS-Ma 073). The specimens were identified using morphological characters of the reproductive system [ 30 , 36 , 37 ]. Additionally, molecular data were used to confirm the taxonomic identity following Guzmán et al [ 30 ].…”

“…The specimens were identified using morphological characters of the reproductive system [ 30 , 36 , 37 ]. Additionally, molecular data were used to confirm the taxonomic identity following Guzmán et al [ 30 ]. For the mitochondrial genome amplification and sequencing, total genomic DNA was extracted from a portion of pedal muscle of a single specimen (IBS-Ma 073–3) using a cetyltrimethyl-ammonium bromide (CTAB) protocol [ 38 ].…”

“…They are also natural and potential intermediate hosts for parasites of the genus Leucochloridium Carus, 1835 (Trematoda) and Angiostrongylus Kamensky, 1905 (Nematoda), respectively [ 24 – 27 ]. Based on morphological characteristics of the reproductive system, six species are currently recognized within Omalonyx [ 28 , 29 ], including Omalonyx unguis (d’Orbigny, 1836) which inhabits the Paraná River basin [ 28 , 30 ].…”

The exotic snail Rumina decollata (Linnaeus, 1758) (Gastropoda, Achatinidae, Rumininae) in Argentina: new records, range extension, and areas of origin of Argentine populations

“…Partial sequences of the mitochondrial COI marker were amplified by PCR using the universal primers LCO1490 and HCO2198 (Folmer et al 1994). PCR master mix and thermal profile were conducted as in Guzmán et al (2018). The PCR products were purified with an AccuPrep PCR Purification Kit (Bioneer, Korea), and bidirectionally sequenced by Macrogen Inc. (Seoul, Korea).…”