First record of potato cyst nematode Globodera rostochiensis in Indonesia

Indarti, Siwi; Rtp, Bambang; Mulyadi, .; Triman, B.

doi:10.1071/ap04018

Cited by 19 publications

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“…It has spread from Europe to tropical and subtropical regions of Asia, such as Pakistan, India, Philippines (Jatala and Bridge 1990). In March 2003, potato cyst nematodes were found in East Java, Indonesia (Indarti et al 2004). In further investigations we have found the nematode in Central Java and West Java and now it is a damaging pathogen in potato plantations in Indonesia.…”

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Identification of potato cyst nematode in Indonesia by polymerase chain reaction

Lisnawita

Supramana

Suastika

2012

Australasian Plant Dis. Notes

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Polymerase chain reaction (PCR) with specific primers was used to amplify the ITS-rDNA regions of potato cyst nematodes (PCN) in soil samples from Indonesia. Amplified DNA fragments showed that a single species, Globodera rostochiensis, with fragment size 238 bp occurred in East Java, and West Java, while mixed populations of G. rostochiensis and G. pallida with fragment size 391 bp were only found in Central Java. Keywords Globodera rostochiensis . G. pallidaPotato Cyst Nematode (PCN) is the most important plant parasitic nematode of potatoes (Solanum tuberosum). It has spread from Europe to tropical and subtropical regions of Asia, such as Pakistan, India, Philippines (Jatala and Bridge 1990). In March 2003, potato cyst nematodes were found in East Java, Indonesia (Indarti et al. 2004). In further investigations we have found the nematode in Central Java and West Java and now it is a damaging pathogen in potato plantations in Indonesia. To control PCN effectively, it is important to identify its species and the pathotypes. Traditionally, routine soil sampling and identification of PCN in Indonesia has been based on examination of morphological features. However, this is time-consuming and frequently unreliable because of morphological similarity of the two PCN species, Globodera rostochiensis and G. pallida.Identification by molecular techniques, for example DNA finger printing, offers a more rapid and precise identification of PCN species. Here, we report studies conducted to identify Indonesian populations of PCN by polymerase chain reaction (PCR).A soil survey was conducted at potato plantations in 24 regions of Java Island. A total of 40 soil samples were collected from these regions. Twenty samples were found to be infested with PCN in 11 regions (Table 1). Soil samples consisting of 100 cores of 15-20 g of soil were taken in a grid pattern throughout the plantations (EPPO 1998). Each 1,500-2,000 g composite sample was collected from soil down to a depth of approximately 100 mm, placed in a plastic bag and was labelled with the producer's name, field location, collection date, GPS coordinates, and sample number for laboratory identification. Samples were put into coolers for storage and transportation from the field and were submitted for processing to the Laboratory of Nematology, Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University, Bogor. Cysts were collected from 100 g soil sub-samples using a pair of sieves (840 and 250 mesh size) (Sheperd 1985) and then stored in Eppendorf tubes at room temperature.DNA was extracted using the modified method described by Subbotin et al. (2001) and Fullaondo et al. (1999). Five cysts from each region were collected randomly into an Eppendorf tube filled with 150 μl lyses buffer (125 mM KC; 25 mM Tris-HCl, pH 8.0; 3.75 mM MgCl2; 2.5 mM DTT; 1.125 % Tween 20 and 0.025 % gelatin) and 5 μl Proteinase K (600 μg/ml) (USB, UK) and were crushed using a plastic pistil. Following consecutive incubation periods at 65°C for 1 h and 95°C...

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confidence: 96%

Identification of potato cyst nematode in Indonesia by polymerase chain reaction

Lisnawita

Supramana

Suastika

2012

Australasian Plant Dis. Notes

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“…Remote deployment of PCN might be happened through planting of potato seeds, moving of plants and machinery and usage of infected soil and water (Ibrahim et al, 2003;Wang et al, 2001). New incursions of potato cyst nematode have also been reported from Indonesia (Indarti et al, 2004;Nugrahana et al, 2017) and Egypt (Ibrahim et al, 2017) in potato areas located at higher altitudes. These incursions of PCN in new areas indicate that pest could be established in temperate areas of Asian and African countries.…”

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confidence: 96%

Studies on potato cyst nematode (Globodera spp.) detection and soil health status in potato core area of Punjab, Pakistan

et al. 2019

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Potato (Solanum tuberosum) is considered as staple food and ranks fourth after wheat, rice and maize in Pakistan. Many biotic constraints affect potato production as potato cyst nematodes (Globodera spp.) are considered to be the most important quarantine pests of potato posing serious potential economic consequences throughout the world. Two hundred soil samples comprising of Okara, Sahiwal, Kasur and Pakpattan districts were analyzed by floatation technique at Plant Pathology Research Institute, Faisalabad and were found free from Potato Cyst Nematodes (PCN) infestation. Soil health study was also carried out following standard analytical techniques and soil health indicators for arable land like organic matter, extractable potash, available phosphorus and microbial populations were found in sufficient levels to ensure healthy crop production.

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“…Patogen ini telah menyebar luas dari Eropa ke daerah subtropika, seperti Pakistan, India, dan Filipina (Jatala dan Bridge 1990). Di Indonesia, NSK pertama kali ditemukan pada bulan Maret 2003 di Desa Tulungrejo, Kecamatan Bumiaji, Kota Batu, Malang, Jawa Timur (Indarti et al 2004), selanjutnya NSK ditemukan di Jawa Tengah dan Jawa Barat. Lisnawita et al (2012) telah mengidentifikasi secara molekul spesies nematoda di Jawa Timur dan Jawa Barat sebagai Globodera rostochiensis, sedangkan nematoda di Jawa Tengah teridentifikasi sebagai G. rostochiensis dan G. pallida.…”

Section: Pendahuluanunclassified

Sebaran Nematoda Sista Kentang di Wonosobo dan Banjarnegara, Jawa Tengah

Syafii

Lisnawita

Hasanudin

2018

J Fitopatol Indones

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Distribution of Potato Cyst Nematode in Wonosobo and Banjarnegara, Central JavaPotato cyst nematode (PCN), Globodera spp., is the primary pathogens that infect potato plants and has been distributed world wide. This study aimed to determine the geographic distribution and species of PCN that infect potato plants in Wonosobo and Banjarnegara, Central Java through a survey, morphological and molecular approaches. Morphological characters were observed among cyst (fenestra diameter, distance from anus to the fenestra, and the Graneks ratio, eggs (length and width of eggs), and the juvenile stage 2 (J2) (length and width of the body, the length and width of the head, the length of the stylet, knob type, as well as the length of the tail). While the molecular identification by polymerase chain reaction is based on the amplification of the internal area transcribed spacers using G. rostochiensis PITSr3 specific primer, G. pallida PITSp4 specific primer and universal nematode primer ITS5. Twelve isolates of PCN, i.e. 5 isolates from Wonosobo and 7 isolates from Banjarnegara were used in this study. The results showed that all isolates were identified as single species, G. rostochiensis.

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First record of potato cyst nematode Globodera rostochiensis in Indonesia

Cited by 19 publications

References 1 publication

Identification of potato cyst nematode in Indonesia by polymerase chain reaction

Identification of potato cyst nematode in Indonesia by polymerase chain reaction

Studies on potato cyst nematode (Globodera spp.) detection and soil health status in potato core area of Punjab, Pakistan

Sebaran Nematoda Sista Kentang di Wonosobo dan Banjarnegara, Jawa Tengah

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