First record of natural vertical transmission of dengue virus in Aedes aegypti from Cuba

Gutiérrez-Bugallo, Gladys; Rodríguez-Roche, Rosmari; Díaz, Gisell; Vázquez, Antonio A.; Álvarez, Mayling; Rodríguez, Magdalena; Bisset, Juan A.; Guzmán, María G.

doi:10.1016/j.actatropica.2017.07.012

Cited by 22 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Vertical transmission has been reported in A . aegypti under experimental [ 9 , 10 ] and natural conditions in different locationsin the world such as Myanmar [ 11 ], Mexico [ 12 ], Brazil [ 13 ] and Cuba [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Dengue virus in Aedes aegypti and Aedes albopictus in urban areas in the state of Rio Grande do Norte, Brazil: Importance of virological and entomological surveillance

et al. 2018

View full text Add to dashboard Cite

BackgroundVector control remains the sole effective method to prevent dengue virus (DENV) transmission, although a vaccine for dengue has recently become available and testing of its efficacy and coverage is being performed in multiple places. Entomological surveillance is a key factor in alerting authorities to possible outbreaks, but until now natural DENV infection of mosquito populations has been scarcely used as an early warning system to monitor fluctuating prevalence of infected mosquitoes. The purpose of this study was to determine the burden of adult and larval/pupae of Aedes aegypti and Aedes albopictus with DENV in urban areas in the state of Rio Grande do Norte, Brazil.Methodology/Principal findingsImmature insect forms (larvae and pupae) were collected from April 2011 to March 2012, whereas the collection of adults was conducted along 3 years: May 2011 to April 2014. Total RNAs of the samples were extracted and the nested reverse transcriptase PCR assay for detecting and typing DENV was performed. Of the 1333 immature insects collected during the study period, 1186 (89%) were A. aegypti and 147 (11%) A. albopictus. DENV-4 was identified in pools of A. aegypti larvae. The rate of DENV infection in immature A. aegypti was expressed as MIR = 3.37. DENV wasnot detected in immature A. albopictus. A total of 1360 adult female mosquitoes of the Aedes genus were captured from May 2011 to April 2014. Of this total, 1293 were A. aegypti (95%) and 67 were A. albopictus (5%). From the 130 pools studied, 27 (20.7%) were positive for DENV. DENV-1 was identified in 2/27 (7.4%) pools; 1of A. albopictus and 1 of A. aegypti. DENV-2 was identified in only 1/27 (3.7%) A. aegypti pools. DENV-4 was the most prevalent, identified in 24/27 (88.8%) of the positive pools, with 19 being of A. aegypti and 5 of A. albopictus pools. The minimum infection rate for adults of the Aedes genus was 19.8, considering both A. aegypti and A. albopictus.Conclusions/SignificanceThis work represents the most complete study to date on the interaction between dengue viruses and Aedes mosquitoes in the State of Rio Grande do Norte, and raises important questions about a possible role of A. albopictus in the transmission of dengue virus in Brazil.

show abstract

Section: Introductionmentioning

confidence: 99%

Dengue virus in Aedes aegypti and Aedes albopictus in urban areas in the state of Rio Grande do Norte, Brazil: Importance of virological and entomological surveillance

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Transmisi transovarial merupakan aspek yang sangat penting dalam menjaga virus dengue selama masa inter-epidemic di alam, 14 saat kondisi lingkungan tidak memungkinkan untuk terjadinya transmisi horizontal. 28 Umniyati menyatakan bahwa telur-telur yang diletakkan pada akhir musim hujan sebelumnya akan menetas pada awal musim hujan berikutnya, jika telur tersebut berasal dari induk infeksius, maka sebagian telur tersebut berpeluang untuk terinfeksi secara transovarial selama stadium telur yang berlangsung selama berbulanbulan. 11 Telur yang menetas pada musim hujan berikutnya tersebut berpeluang besar menjadi larva yang infeksius dan selanjutnya menjadi nyamuk yang infeksius virus dengue walaupun belum pernah menggigit dan menghisap darah inang infeksius virus dengue.…”

Section: Pembahasanunclassified

Pengaruh Lama Penyimpanan Telur terhadap Transovarial Infection Rate Virus DEN-3 pada Nyamuk Aedes aegypti

Wahono¹,

Umniyati

Satoto

2019

blb

View full text Add to dashboard Cite

Transovarial transmission of dengue virus is important phenomenon causes dengue virus survival during inter-epidemic period. The purpose of this study was to analyze effect of egg storage duration on DEN-3 virus Transovarial Infection Rate (TIR) in Aedes aegypti mosquitoes. This was laboratory research with experimental design. Ae. aegypti was infected with the DEN-3 virus per-orally and allowed to undergo its gonotrophic cycle. Eggs stored at room temperature (25±3oC) and relative humidity (70±5%) for 0, 1, 2 and 3 months. Hatched eggs then colonized until become adult mosquito. Sample was taken from colonies at 4, 8, 16 days old. Headsquash of mosquitoes was preparedwith Streptavidin Biotin Peroxidase Complex (SBPC) immunocytochemical method using DSSE 10 monoclonal antibody and detected the presence virus antigen. Transovarial infection rate was determined by counting percentage of positive samples. Data were analyzed by linear regression test. Result of this study showed that in 4, 8, 16 samples showed lowest TIR on 0 month storage (33,33%, 33,33%; 40,00%) and highest TIR observed on 2 month storage (76,67%; 66,67%, 76,67%). Statistical test showed significantly different result (p = 0,013) with α = 0,05 and R2 = 0,476. The duration of egg storage effect on DEN-3 virus TIR in Ae. aegypti mosquito with 47.6% contribution.

show abstract

“…Vertical transmission has been reported for Rift Valley fever virus [15], LACV, the Murray Valley encephalitis virus (MVEV), JEV, EEEV [4], ZIKV, and DENV [16-18]. Although vertical transmission in A. aegypti has been reported only for ZIKV [19], DENV [16, 20], and CHIKV [21-24]. …”

Section: Mosquitoes As Vectorsmentioning

confidence: 99%

Molecular Basis for Arbovirus Transmission by <b><i>Aedes aegypti</i></b> Mosquitoes

et al. 2018

View full text Add to dashboard Cite

Mosquitoes are considered the most important vectors for the transmission of pathogens to humans. Aedes aegypti is a unique species, not only by its highly anthropophilic and peridomestic habits but also because it can transmit an important variety of pathogenic viruses. Examples are dengue, yellow fever, chikungunya, Zika, and Mayaro viruses. After ingesting viremic blood, a wide range of mechanisms are activated in the mosquito to counteract viral infection. Nevertheless, these arboviruses possess strategies to overcome barriers in the mosquito and eventually reach the salivary glands to continue the transmission cycle. However, the infection and eventual transmission of arbovirus depends on multiple factors. The current review focuses in detail on the anatomic, physiological, and molecular characteristics of the mosquito A. aegypti that participate in response to a viral infection. In the past decades, the awareness of the importance of this mosquito as a disease vector and its impact on human health was largely recognized. We need to improve our comprehension of molecular mechanisms that determine the outcome of successful virus replication or control of infection for each arbovirus in the vector; this could lead to the design of effective control strategies in the future.

show abstract

First record of natural vertical transmission of dengue virus in Aedes aegypti from Cuba

Cited by 22 publications

References 23 publications

Dengue virus in Aedes aegypti and Aedes albopictus in urban areas in the state of Rio Grande do Norte, Brazil: Importance of virological and entomological surveillance

Dengue virus in Aedes aegypti and Aedes albopictus in urban areas in the state of Rio Grande do Norte, Brazil: Importance of virological and entomological surveillance

Pengaruh Lama Penyimpanan Telur terhadap Transovarial Infection Rate Virus DEN-3 pada Nyamuk Aedes aegypti

Molecular Basis for Arbovirus Transmission by <b><i>Aedes aegypti</i></b> Mosquitoes

Contact Info

Product

Resources

About