First record of Longidorus intermedius Kozlowska and Seinhorst, 1979 (Nematoda: Longidoridae) from the Czech Republic

Abstract

122 SummaryLongidorus intermedius Kozlowska and Seinhorst, 1979 was recorded in the Czech Republic for the first time in the rhizosphere of Robinia pseudoacacia at Dolní Bojanovice and Carpinus betulus -Euonymus europaeus -Quercus sp. at Strážovice. The morphological and morphometrical characteristics are studied.

Morphological and chorological characterization of Longidorus intermedius Kozlowska & Seinhorst, 1979 firstly reported from Ukraine with the comments on Longidorus elongatus (de Man, 1876) Thorne & Swanger, 1936

“…Tail ventrally more curved than in female, bluntly conoid with a rounded terminus. Juveniles (Table 2) The analysis of morphometrics revealed presence of only three juvenile developmental stages, same as in previous publications [2,7,9,10]. Morphologically juveniles are similar to adults.…”

“…In comparison to type population from the Netherlands, they have higher values of b Studies populations are most similar to populations from Bulgaria, but differ by slightly shorter tail (mean 38.1; 38.4; 38.5; 42 vs 41; 42; 43; 43; 44; 47 µm) and lower c′ value (mean 1.09; 1.12; 1.15; 1.22 vs 1.2; 1.2; 1.2; 1.3; 1.3; 1.4) [10]. They are more similar to Italian, Spanish and Czech populations [9,10].…”

“…This species was described from Netherlands and Germany from the rhizosphere of Quercus robur L. [7]. It was also previously reported from Belgium, Italy, Spain, countries of Balkan Peninsula and Central Europe and from the European part of Russia [2,9,10,12]. Most populations of L. intermedius were collected in oak forests from the rhizosphere of Quercus spp.…”

“…Morphology of Longidorus intermedius(1,3,5,7,8) and L. elongatus(2,4,6,9) from Ukraine: 1, 2female anterior regions; 3, 4 -male tail regions; 5, 6 -vulval regions; 7 -female anterior region with focus on aphidial fovea; 8, 9 -female tails Рис. Морфологічні особливості українських популяцій Longidorus intermedius(1,3,5,7,8) і L. elongatus (2, 4, 6, 9): 1, 2 -передня частина тіла самок; 3, 4 -хвостова частина тіла самців; 5, 6 -вульва; 7 -передня частина тіла самки з акцентом на амфід; 8, 9 -хвости самок…”

The morphological and molecular identity of Longidorus piceicola Lišková, Robbins & Brown, 1997 from Romania (Nematoda, Dorylaimida)

“…Based on the molecular and morphological characterization L. piceicola is closely related to L. intermedius : however, it differs in having a much longer odontostyle (151–169 μm in the type population and reported range of 144–183 μm vs 105–118 μm and 97–121 μm, respectively), generally longer body (4.22–5.97 mm in the type population and reported range of 4.42–7.99 mm vs 3.6–4.5 mm and 3.11–5.4 mm, respectively) and bigger anterior end – guide ring distance (37–45 μm in the type population and a range of 34–46 μm vs 25–34 μm and 27–36 μm, respectively); a wider lip region (14–18 vs 11–12.5 μm), more ventromedian supplements (11 vs 5–7) in the males, and four vs three juvenile stages (Lišková et al 1997, Peneva et al 2001, Barsi and Lamberti 2001, Kumari et al 2006, Kornobis and Peneva 2011, Gutiérrez-Gutiérrez et al 2013). Sequence and SNPs analyses of the D2-D3 rDNA region of L. piceicola and L. intermedius populations showed three transitions and four transversions that can be used to s differentiate between both species.…”