First record of<i>Pythium tracheiphilum</i>associated with lettuce wilt and leaf blight in Australia

Kumar, Sanjay; Ray, Jeffery D.; Davison, Elaine; Cunnington, J. H.; Alwis, S. de

doi:10.1071/dn07004

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…Pythium tracheiphilum Matta was first described in 1965 as a pathogen of lettuce ( Lactuca sativa L.) in Italy (Matta, 1965). Subsequently, it was reported infecting lettuce in the Netherlands (Blok & van der Plaats‐Niterink, 1978), then in North America, the United Kingdom, Australia, Spain and Argentina (González et al., 2004; Hall, 1989; Kiehr et al., 2015; Kumar et al., 2007; Nordskog et al., 2008). This species of Pythium is a soilborne oomycete that causes vein necrosis, stunting, wilting and root, stem and leaf rot in lettuce.…”

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Pythium tracheiphilum causing leaf rot and wilting in lettuce in Brasília‐DF, Brazil

Barboza,

Lopes,

Pires‐Zottarelli

et al. 2024

Journal of Phytopathology

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Pythium tracheiphilum is a narrow host range oomycete first described infecting lettuce in Italy. Subsequently, the pathogen was reported in many other countries. From 2015 to 2019, Pythium isolates were recovered from lettuce plants with symptoms of leaf rot, wilting and vascular necrosis, collected from commercial fields in different rural localities of Brasilia‐DF, Brazil. The isolates were identified as P. tracheiphilum based on morphological and molecular genetic analysis. Pathogenicity tests conducted with four P. tracheiphilum isolates confirmed that the isolates were pathogenic to detached leaves of a loose‐hearted lettuce cultivar. This is the first report of P. tracheiphilum infecting lettuces in Brazil.

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Section: Figurementioning

confidence: 99%

Pythium tracheiphilum causing leaf rot and wilting in lettuce in Brasília‐DF, Brazil

Barboza,

Lopes,

Pires‐Zottarelli

et al. 2024

Journal of Phytopathology

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“…This is the first report of vascular necrosis caused by P. tracheiphilum on endive in Spain. This pathogen had previously been reported affecting endive in Italy (Sportelli 2007) and was also isolated in Australia from symptomatic plants of chicory, which is botanically closely related to endive, although no further studies to accomplish Koch's postulates were developed (Kumar et al 2007). In eastern Spain, where different vegetable species are intercropped in rotation every year, no vascular wilt incidence caused by P. tracheiphilum has been reported to date in crops other than lettuce or endive.…”

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confidence: 96%

“…associated with low temperatures and wet seasons (Kumar et al 2007;Tortolero and Sequeira 1978) and include severe wilt and stunting of the plants, as well as necrosis and discoloration of vascular vessels (González et al 2004).…”

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Identification of Pythium tracheiphilum as the causal agent of vascular necrosis of endive (Cichorium endivia) in Spain

et al. 2011

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Pythium isolates were recovered from endive plants (Cichorium endivia) showing vascular necrosis collected from commercial fields located in Castellón province (eastern Spain). They were identified as Pythium tracheiphilum on the basis of their phenotypical and molecular profile. Pathogenicity tests conducted with two P. tracheiphilum isolates, obtained from endive and lettuce (Lactuca sativa), respectively, in this region, confirmed that both isolates were pathogenic to endive, with no significant differences in virulence between them. This is the first report of vascular necrosis caused by P. tracheiphilum on endive in Spain.

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“…Among these, Pythium tracheiphilum Matta was first identified in 1965 to cause lettuce stem rot and vascular necrosis (Matta 1965). It was later identified in the Netherlands to be highly pathogenic to lettuce (Blok and van der Plaats-Niterink 1978) and subsequently reported in North America, the United Kingdom, and Australia (Hall 1989;Kumar et al 2007;Nordskog et al 2008). This is one of the very few Pythium spp.…”

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Detection and Quantification of Pythium tracheiphilum in Soil by Multiplex Real-Time qPCR

et al. 2019

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In Canada, head lettuce (Lactuca sativa capitata) is extensively produced in the muck soils of southwestern Québec. However, yields are increasingly affected by various soilborne pathogens, including Pythium spp., which cause wilt and damping off. In a survey conducted in Québec muck soils in 2010 and 2011, Pythium tracheiphilum Matta was identified as the predominant Pythium sp. in the root of head lettuce showing Pythium stunt symptoms. Therefore, to improve risk assessment and help further understanding of disease epidemiology, a specific and sensitive real-time quantitative polymerase chain reaction (qPCR) assay based on TaqMan-minor groove binder (MGB) technology was developed for P. tracheiphilum. The PCR primers along with a TaqMan-MGB probe were designed from the ribosomal internal transcribed spacer 2 region. A 100-bp product was amplified by PCR from all P. tracheiphilum isolates tested while no PCR product was obtained from 38 other Pythium spp. or from a selection of additional lettuce pathogens tested. In addition to P. tracheiphilum, the assay was multiplexed with an internal control allowing for the individual validation of each PCR. In artificially infested soils, the sensitivity of the qPCR assay was established as 10 oospores/g of dry soil. P. tracheiphilum was not detected in soils in which lettuce has never been grown; however, inoculum ranged from 0 to more than 200,000 oospores/g of dry soil in commercial lettuce fields. Also, disease incidence was positively correlated with inoculum concentration (r = 0.764). The results suggest that inoculum concentration should be considered when making Pythium stunt management decisions. The developed qPCR assay will facilitate reliable detection and quantification of P. tracheiphilum from field soil.

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First record ofPythium tracheiphilumassociated with lettuce wilt and leaf blight in Australia

Abstract: Abstract. Pythium tracheiphilum,

Cited by 5 publications

References 9 publications

Pythium tracheiphilum causing leaf rot and wilting in lettuce in Brasília‐DF, Brazil

Pythium tracheiphilum causing leaf rot and wilting in lettuce in Brasília‐DF, Brazil

Identification of Pythium tracheiphilum as the causal agent of vascular necrosis of endive (Cichorium endivia) in Spain

Detection and Quantification of Pythium tracheiphilum in Soil by Multiplex Real-Time qPCR

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