First record and molecular identification of root-knot nematodes from honeybush tea plants (Cyclopia spp.) in South Africa

Daramola, Fisayo; Malgas, Rhoda; Malan, Antoinette P.

doi:10.1163/15685411-bja10023

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…From earlier reports in the Western Cape province of South Africa, M. javanica can be seen to be more frequently encountered, and pathogenic, in the region. Daramola et al (2020) reported M. javanica and M. hapla from honeybush fields in Western Cape. According to Loubser and Meyer (1987), the presence of the two most important species in South Africa vineyards is determined by the vastly different climatic regions concerned.…”

Section: Discussionmentioning

confidence: 99%

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Occurrence and seasonal changes in the population of root-knot nematodes on honeybush (Cyclopia sp.)

2021

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Summary Root-knot nematodes in the genus Meloidogyne are an important group of plant-parasitic nematodes causing severe damage on agricultural crops worldwide. A study was conducted to identify the species of root-knot nematodes causing damage on honeybush monocultures and to assess the seasonal variations in the nematode population. Soil samples were collected from six experimental sites in Genadendal, Western Cape province of South Africa from 2016 to 2017. DNA was extracted from single-second stage juveniles and species identifi cation was done using species-specifi c sequence-characterised amplifi ed regions (SCAR) primers. Meloidogyne hapla and M. javanica were identifi ed from the sites. Mean population density of the nematodes varied significantly (p < 0.05) in the six sites, with the peak population being recorded in summer of 2017. The study suggests that seasonal variation in temperature and moisture could contribute to changes in the population density of root-knot nematodes in the soil.

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Section: Discussionmentioning

confidence: 99%

“…DNA was extracted from single J2 root-knot larvae obtained from the honeybush samples as described by Daramola et al (2020). The specimen for DNA extraction were placed in a Petri-dish and washed twice with ddH 2 O.…”

Section: Dna Extraction and Polymerase Chain Reactionmentioning

confidence: 99%

Occurrence and seasonal changes in the population of root-knot nematodes on honeybush (Cyclopia sp.)

2021

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“…Criconema mutabile, Meloidogyne hapla, Meloidogyne javanica, Xiphinema oxycaudatumm, and Scutellonema spp. Were identified as being the most prevalent nematodes causing a reduction in yield and economic losses in honeybush production (Daramola et al, 2020(Daramola et al, , 2021.…”

Section: Pest Control and Diseasesmentioning

confidence: 99%

“…Nematodes were initially identified as a possible cause of high honeybush mortality in commercial plantings (Joubert et al., 2007a). Later studies indicated that honeybush is highly susceptible to root‐knot nematodes (Daramola et al, 2020). Criconema mutabile, Meloidogyne hapla, Meloidogyne javanica, Xiphinema oxycaudatumm , and Scutellonema spp.…”

Section: Pest Control and Diseasesmentioning

confidence: 99%

Propagation and cultivation practices of honeybush (Cyclopia spp.) for the sustainable production of an export quality indigenous South African tea

et al. 2022

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Honeybush (Cyclopia) species are endemic to the Fynbos Biome of the Cape Floral Kingdom in South Africa. Traditionally, honeybush foliage is cut, fermented, and sun-dried to make an antioxidant-rich herbal tea. Approximately 80% of honeybush is unsustainably harvested from the wild. All commercial species are indicated on the International Union for Conservation of Nature Red List of Threatened Species. Cultivation is thus required to ensure sufficient volumes of tea are produced. A major limitation to cultivation is the poor rooting responses of the species that regenerate from shoots, C. genistoides and C. intermedia, the latter being the most harvested from the wild. The establishment of clonally propagated material is a challenge for all six of the commercially important Cyclopia species. Research on honeybush has mainly focused on the unique properties contributing to flavor, medicinal value, as well as processing. Much less is known about propagation, nutrient requirements, harvesting procedures, plant improvement, and pest and disease management. This review considers management strategies for the sustainable harvesting of wild honeybush and the potential risks of introducing improved plant material. Cultivation practices and management strategies that promote longevity and enhance yield are defined. The inherent rooting ability of cuttings is discussed. Cultivated stands are mostly established by seedlings which results in diversity of vigor and tea quality. A sustainable market based on high-quality indigenous tea requires the use of clonal material which allows the selection of desired traits and rapid multiplication. Its use would enable improved yield and profitability without compromising tea quality.

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Nematicidal effects of silver nanoparticles (AG-NPs) on the root-knot nematode, Meloidogyne javanica associated with Swiss chard (Beta vulgaris L.)

Daramola,

Lewu,

Nkiko

et al. 2023

Helminthologia

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Summary Root-knot nematodes (RKNs) are important nematode pests, causing huge economic losses on vegetable crops worldwide. A decline in the yield of Swiss chard (Beta vulgaris L.) which was associated with RKNs was observed on an organic vegetable farm in the Western Cape Province of South Africa. Nematodes were extracted from galled plant roots and identified using molecular tools. PCR-based Sequence Characterised Amplified Region (SCAR) primers was used to confirm the specie of the RKN associated with the infected plants. Thereafter, a pot assay was conducted to determine the response of artificially infected Swiss chard plants to varying concentrations of bio-synthesized silver nanoparticle Ag-NP (1 μg/mL, 2 μg/mL, and 3 μg/mL) under controlled conditions. The results of the study showed that Swiss chard is highly susceptible to M. javanica with an egg-laying-female index of >5 in all infected plants. Significantly lower values (at P=0.05) in egg masses (EM), juveniles (J2s), and reproduction factor (RF) of nematodes were recorded on plants treated with 3 μg/mL, indicating a potential for nematode control. A negative correlation was also observed in the number of egg masses, J2s, and RF of the nematodes with increasing concentrations of the Ag-NP. This study confirms that Swiss chard is highly susceptible to M. javanica and demonstrates the potential nematicidal property of Ag-NP in controlling the nematode pest of Swiss chard.

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First record and molecular identification of root-knot nematodes from honeybush tea plants (Cyclopia spp.) in South Africa

Cited by 3 publications

References 8 publications

Occurrence and seasonal changes in the population of root-knot nematodes on honeybush (Cyclopia sp.)

Occurrence and seasonal changes in the population of root-knot nematodes on honeybush (Cyclopia sp.)

Propagation and cultivation practices of honeybush (Cyclopia spp.) for the sustainable production of an export quality indigenous South African tea

Nematicidal effects of silver nanoparticles (AG-NPs) on the root-knot nematode, Meloidogyne javanica associated with Swiss chard (Beta vulgaris L.)

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