First Otoliths/Collagen/Bacterial Cellulose Nanocomposites as a Potential Scaffold for Bone Tissue Regeneration

Olyveira, Gabriel Molina de; Valido, Daisy Pereira; Costa, Lígia Maria Manzine; Góis, Plácia Barreto Prata; Filho, Lauro Xavier; Basmaji, Pierre

doi:10.4236/jbnb.2011.23030

Cited by 29 publications

(8 citation statements)

References 23 publications

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“…The results of this process were vinegar and nanobiocellulose biomass. Bacterial cellulose is produced by the Gram-negative bacterium Gluconacetobacter xylinus and extracted from the culture medium in a pure 3D structure, formed by an ultra-thin network of highly hydrated (3–8 nm) cellulose nanofibers (99% in weight), displaying high molecular weight, high cellulose crystallinity (60–90%), enormous mechanical strength, and full biocompatibility [ 18 , 20 , 22 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

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Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

et al. 2020

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Guided bone regeneration was studied to establish protocols and develop new biomaterials that revealed satisfactory results. The present study aimed to comparatively evaluate the efficiency of the bacterial cellulose membrane (Nanoskin®) and collagen membrane Bio-Gide® in the bone repair of 8-mm critical size defects in rat calvaria. Seventy-two adult male rats were divided into three experimental groups (n = 24): the CG—membrane-free control group (only blood clot, negative control), BG—porcine collagen membrane group (Bio-Guide®, positive control), and BC—bacterial cellulose membrane group (experimental group). The comparison periods were 7, 15, 30, and 60 days postoperatively. Histological, histometric, and immunohistochemical analyses were performed. The quantitative data were subjected to 2-way ANOVA and Tukey’s post-test, and p < 0.05 was considered significant. At 30 and 60 days postoperatively, the BG group showed more healing of the surgical wound than the other groups, with a high amount of newly formed bone (p < 0.001), while the BC group showed mature connective tissue filling the defect. The inflammatory cell count at postoperative days 7 and 15 was higher in the BC group than in the BG group (Tukey’s test, p = 0.006). At postoperative days 30 and 60, the area of new bone formed was greater in the BG group than in the other groups (p < 0.001). Immunohistochemical analysis showed moderate and intense immunolabeling of osteocalcin and osteopontin at postoperative day 60 in the BG and BC groups. Thus, despite the promising application of the BC membrane in soft-tissue repair, it did not induce bone repair in rat calvaria.

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Section: Methodsmentioning

confidence: 99%

“…an ultra-thin network of highly hydrated (3-8 nm) cellulose nanofibers (99% in weight), displaying high molecular weight, high cellulose crystallinity (60-90%), enormous mechanical strength, and full biocompatibility [18,20,[22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

et al. 2020

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“…As these human proteins are closely related to the process of bone mineralization, it is suggested that otoliths could potentially work as a biomineral in bone neoformation. In addition, evidence that otoliths derived from Cynoscion acoupa carried in collagen/bacterial cellulose nanocomposites can induce bone neoformation has been previously reported [11].…”

Section: Introductionmentioning

confidence: 91%

Otoliths-composed gelatin/sodium alginate scaffolds for bone regeneration

Valido

Júnior

Andrade

et al. 2020

Drug Deliv. and Transl. Res.

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Evidence that otoliths, mineral-rich limestone concrescences present in the inner ear of bone fishes, can accelerate bone formation in vivo has been previously reported. The goal of this work was the development, characterization, and evaluation of the cytocompatibility of otoliths-incorporated sodium alginate and gelatin scaffolds. Cynoscion acoupa-derived otoliths were characterized by X-ray fluorescence spectrometry (FRX), particle size, free lime, and weight loss by calcination. Furthermore, otoliths were incorporated into sodium alginate (ALG/OTL-s) or gelatin (GEL/OTL-s) scaffolds, previously developed by freeze-drying. Then, the scaffolds were characterized by thermogravimetric analysis (TGA/DTG), differential scanning calorimetry (DSC), infrared spectroscopy with Fourier transform (FTIR), swelling tests, and scanning electron microscopy (SEM). Cytotoxicity assays were run against J774.G8 macrophages and MC3T3-E1 osteoblasts. Data obtained from TGA/DTG, DSC, and FTIR analyses confirmed the interaction between otoliths and the polymeric scaffolds. SEM showed the homogeneous porous 3D structure rich in otolith micro-fragments in both scaffolds. Swelling of the GEL/OTL-s (63.54 ± 3.0%) was greater than of ALG/ OTL-s (13.36 ± 9.9%) (p < 0.001). The viability of J774.G8 macrophages treated with both scaffolds was statistically similar to the group treated with DMEM only (p > 0.05) and significantly higher than that treated with Triton-X (p < 0.01) at 72 h. Both scaffolds showed approximately 100% growth of MC3T3-E1 osteoblasts by 24 h, similarly to control (p > 0.05). However, by 48 h, only ALG/OTL-s showed growth similar to control (p > 0.05), whereas GEL/OTL showed a significantly lower growth index (p < 0.05). In conclusion, the physicochemical profiles suggest proper interaction between the otoliths and the two developed polymeric 3D scaffolds. Moreover, both materials showed cytocompatibility with J774.G8 macrophages but the growth of MC3T3-E1 osteoblasts was higher when exposed to ALG/OTL-s. These data suggest that sodium alginate/otoliths scaffolds are potential biomaterials to be used in bone regeneration applications.

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“…Olyveira et al [157] Glycidyltrimethylammonium chloride (GTMAC) BC/GTMAC films showed a significant increase in cell attachment and spreading compared to unmodified membranes. Courtenay et al [158] BC and collagen associated with osteogenic growth peptide.…”

Section: General Biomedical Applicationsmentioning

confidence: 99%