First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in Southern Brazil

Gusatti, Carolina de Souza; Bertholdo, Lauren Martins; Otton, Letícia Muner; Marchetti, Desirèe Padilha; Ferreira, Alessandra Einsfeld; Corção, Gertrudes

doi:10.1590/s1517-83822012000100027

Cited by 12 publications

(5 citation statements)

References 14 publications

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“…Eighty-one tracheal aspirates were isolated from different wards of al-Zahra hospitals in Isfahan from 2016 to 2017. Initial identification was performed by culture and biochemical tests and then final confirmation was conducted using PCR of the bla oxa-51 gene which is inherent in A. baumannii [1,2]. This study was evaluated and approved by the Ethics Committee of Isfahan University of Medical Sciences (project no.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

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Investigation of carbapenem resistant Acinetobacter baumannii ST2 in Iran

Rezaei

Fazeli

Faghri

2021

Acta Microbiologica Et Immunologica Hungarica

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This study investigated carbapenem resistance among Acinetobacter baumannii isolated from respiratory specimens. Epidemiological relationship of the isolates was also evaluated. In this study, 81 respiratory specimens of A. baumannii from AL Zahra Hospital were confirmed by phenotypic and genotypic methods. Antimicrobial susceptibility was performed by disc diffusion method. Carbapenem resistance genes were identified by PCR. The isolates were typed by RAPD-PCR and multilocus sequence typing (MLST) methods. All isolates were resistant to imipenem and 80 isolates to meropenem. Frequency of oxacillinase genes was as follows: blaOXA-23 gene was positive in 74 (91.3%), blaOXA-24 gene in 50 (61.7%) and blaOXA-58 was not found in any isolates. On the other hand 22 (27.2%) isolates contained blaIMP-1, 3 (3.7%) isolates contained blaIMP-2 gene, 5 (6.2%) isolates contained blaVIM-1, 4 (5%) isolates had blaVIM-2 and none of the isolates had blaSIM-1 gene. RAPD-PCR typing identified 16 different patterns, with one pattern being the most frequent one in 26 isolates. In MLST 6 different sequence types were identified, the most predominant being ST2 belonging to clonal complex 2. The results of this study showed high resistance to carbapenems as well as high abundance of oxacillinase genes.

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Section: Bacterial Isolatesmentioning

confidence: 99%

“…One of the most important nosocomial infections usually caused by endotracheal tube and mechanical ventilation is pneumonia. The incidence of this disease is 6-52% and is associated with high mortality and morbidity, especially in the intensive care unit [1].…”

Section: Introductionmentioning

confidence: 99%

Investigation of carbapenem resistant Acinetobacter baumannii ST2 in Iran

Rezaei

Fazeli

Faghri

2021

Acta Microbiologica Et Immunologica Hungarica

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“…These carbapenemases are encoded by the bla OXA genes, which are usually carried by highly transmissible plasmids ( Nguyen and Joshi, 2021 ). In Brazil, bla OXA-23 is the most widely disseminated OXA-type carbapenemase in CRAB, followed by bla OXA-143 , while bla OXA-58 is less common ( de Souza Gusatti et al, 2012 ; de Oliveira et al, 2019 ; Matos et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…OXA-58 was first described in 2003 in France; since then, it has been widely reported worldwide, being associated with outbreaks of nosocomial infections ( Moro et al, 2008 ; Ozen et al, 2009 ; de Souza Gusatti et al, 2012 ). OXA-58 hydrolyzes carbapenems at low levels; however, expression can be increased by the presence of insertion sequences, such as IS Aba3 , resulting in resistance to carbapenems ( Walther-Rasmussen and Høiby, 2006 ; Evans and Amyes, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic study of Acinetobacter baumannii strains co-harboring blaOXA-58 and blaNDM-1 reveals a large multidrug-resistant plasmid encoding these carbapenemases in Brazil

Rodrigues,

Silveira,

Pribul

et al. 2024

Front. Microbiol.

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IntroductionAcinetobacter baumannii contributes significantly to the global issue of multidrug-resistant (MDR) nosocomial infections. Often, these strains demonstrate resistance to carbapenems (MDR-CRAB), the first-line treatment for infections instigated by MDR A. baumannii. Our study focused on the antimicrobial susceptibility and genomic sequences related to plasmids from 12 clinical isolates of A. baumannii that carry both the blaOXA-58 and blaNDM-1 carbapenemase genes.MethodsWhole-genome sequencing with long-read technology was employed for the characterization of an A. baumannii plasmid that harbors the blaOXA-58 and blaNDM-1 genes. The location of the blaOXA-58 and blaNDM-1 genes was confirmed through Southern blot hybridization assays. Antimicrobial susceptibility tests were conducted, and molecular characterization was performed using PCR and PFGE.ResultsMultilocus Sequence Typing analysis revealed considerable genetic diversity among blaOXA-58 and blaNDM-1 positive strains in Brazil. It was confirmed that these genes were located on a plasmid larger than 300 kb in isolates from the same hospital, which also carry other antimicrobial resistance genes. Different genetic contexts were observed for the co-occurrence of these carbapenemase-encoding genes in Brazilian strains.DiscussionThe propagation of blaOXA-58 and blaNDM-1 genes on the same plasmid, which also carries other resistance determinants, could potentially lead to the emergence of bacterial strains resistant to multiple classes of antimicrobials. Therefore, the characterization of these strains is of paramount importance for monitoring resistance evolution, curbing their rapid global dissemination, averting outbreaks, and optimizing therapy.

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“…baumannii, um patógeno oportunista, frequentemente envolvido em infecções nosocomias, e que possui grande capacidade de adquirir resistência a antibióticos de escolha para tratar as infecções causadas pelo mesmo. Seu mecanismo de resistência mais frequente, aos carbapenêmicos, também se dá por β -lactamases, especificamente pelas OXA-carbapenemases da classe D de Ambler [25,26] .…”

Section: Introductionunclassified

Incidência de infecções relacionadas à assistência à saúde e perfil de sensibilidade de Klebsiella pneumoniae e Acinetobacter baumannii no período pré-pandêmico e durante a pandemia de COVID-19 em unidade de terapia intensiva adulto

Messias

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A resistência antimicrobiana constitui uma problemática dos serviços de saúde, principalmente no cenário das Unidades de Terapia Intensiva (UTI), onde, além dos pacientes apresentarem maior grau de invasão em termos de cuidado médico, tais como acessos venosos centrais, tubos endotraqueais e sondas nasoentéricas, também dependem do manejo direto pela equipe para atividades básicas de vida como, por exemplo, banho e dieta assistida. Toda manipulação do paciente com a finalidade diagnóstica e terapêutica, pode implicar em maior risco de adquirir Infecções Relacionadas à Assistência à Saúde (IRAS) durante o período de internação. As medidas preventivas são norteadas pela Comissão de Controle de Infecção Hospitalar (CCIH) que analisa a incidência e a prevalência destas infecções por topografia, além de realizar levantamento dos micro-organismos multidroga resistentes (MDR) dentro destas unidades de assistência. Com o cenário da pandemia pela COVID-19, houve a extensão dos leitos de terapia intensiva, agravamento das IRAS, e um aumento considerável da ocorrência de microorganismos multidroga resistentes (MDR), especialmente as bactérias gram-negativas, tais como Klebsiella pneumoniae e Acinetobacter baumannii. O objetivo deste estudo é avaliar o impacto da pandemia pela COVID-19 na incidência das IRAS e a mudança do perfil de resistência antimicrobiano de duas bactérias de importância epidemiológica (Klebsiella pneumoniae e Acinetobacter baumannii ) em uma UTI geral que, posteriormente foi destinada à pacientes com COVID-19. Este é um estudo tipo coorte, retrospectivo, que será desenvolvido em uma Unidade de Terapia Intensiva Adulto do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto (HCFMRP-USP), Unidade Campus.Para avaliação proposta, o estudo foi divido em período Pré-pandêmico (01 de Setembro de 2018 à 28 de Fevereiro de 2020) e período Pandêmico (01 de Março de 2020 à 31 de Agosto de 2021), com inclusão dos pacientes internados na Unidade de Terapia Intensiva. Para estudo da prevalência de Klebsiella pneumoniae e Acinetobacter baumannii, e seus respectivos perfis de resistência antimicrobiana, foram utilizadas culturas coletadas a partir dos pacientes internados. Paralelamente, para avaliação da Densidade de Incidência das Infecções Relacionadas à Assistência à Saúde, foram extraídos dados das planilhas da Comissão de Controle de Infecção Hospitalar (CCIH), referentes a cada período. A análise estatística foi composta por análise comparativa entrevariáveis qualitativas e quantitativas, através dos testes de Qui-quadrado de independência e teste U de Mann-Whitney, respectivamente. Entre as infecções, viu-se aumento na Densidade de Incidência apenas da Pneumonia Associada à Ventilação Mecânica (0.0 para 5.69 pneumonias/1.000 ventilações-dia). Em relação as bactérias de interesse, houve aumento da prevalência, tanto de Klebsiella pneumoniae (8.2% para 21.9% culturas positivas/total de pacientes), quanto para Acinetobacter baumannii (7.5% para 21.7% culturas positivas/total de pacientes), em conjunto ...

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First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in Southern Brazil

Cited by 12 publications

References 14 publications

Investigation of carbapenem resistant Acinetobacter baumannii ST2 in Iran

Investigation of carbapenem resistant Acinetobacter baumannii ST2 in Iran

Genomic study of Acinetobacter baumannii strains co-harboring blaOXA-58 and blaNDM-1 reveals a large multidrug-resistant plasmid encoding these carbapenemases in Brazil

Incidência de infecções relacionadas à assistência à saúde e perfil de sensibilidade de Klebsiella pneumoniae e Acinetobacter baumannii no período pré-pandêmico e durante a pandemia de COVID-19 em unidade de terapia intensiva adulto

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