First Nuclear DNA Amounts in more than 300 Angiosperms

Zonneveld, B. J. M.; Leitch, Ilia J.; Bennett, M. D.

doi:10.1093/aob/mci170

Cited by 227 publications

(190 citation statements)

References 20 publications

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“…CO distribution along chromosome 3B: The construction of a dense genetic map of wheat chromosome 3B comprising 102 markers well distributed across the genetic and deletion maps was challenging in a species with a large genome of 17 Gb (Zonneveld et al 2005), consisting of $90% of repeated sequence (Flavell and Smith 1977;Li et al 2004;Paux et al 2006), for which no genome sequence is available and showing a low level of polymorphism (Chao et al 1989;Kam-Morgan et al 1989;Cadalen et al 1997). The development of the CsRe genetic map of chromosome 3B was facilitated greatly by the construction of a BAC-based physical map (Paux et al 2008) that provided a large source of markers well distributed along the minimal tiling path (MTP) of this chromosome.…”

Section: Discussionmentioning

confidence: 99%

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

et al. 2009

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In wheat (Triticum aestivum L.), the crossover (CO) frequency increases gradually from the centromeres to the telomeres. However, little is known about the factors affecting both the distribution and the intensity of recombination along this gradient. To investigate this, we studied in detail the pattern of CO along chromosome 3B of bread wheat. A dense reference genetic map comprising 102 markers homogeneously distributed along the chromosome was compared to a physical deletion map. Most of the COs (90%) occurred in the distal subtelomeric regions that represent 40% of the chromosome. About 27% of the proximal regions surrounding the centromere showed a very weak CO frequency with only three COs found in the 752 gametes studied. Moreover, we observed a clear decrease of CO frequency on the distal region of the short arm. Finally, the intensity of interference was assessed for the first time in wheat using a Gamma model. The results showed m values of 1.2 for male recombination and 3.5 for female recombination, suggesting positive interference along wheat chromosome 3B.

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Section: Discussionmentioning

confidence: 99%

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

et al. 2009

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“…These methods use standards of known molecular weight or reference species with a defined nuclear DNA mass (Zonneveld et al, 2005). The total assembled scaffold length (in Mb) can then be expressed as a fraction of the molecular weight of the nuclear DNA (in pg) using the standard average molecular weight of 1 pg per 978 Mb for the conversion.…”

Section: Estimating the Completeness Of A Genome Assemblymentioning

confidence: 99%

Are We There Yet? Reliably Estimating the Completeness of Plant Genome Sequences

2016

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“…Experimental data were analyzed and coefficient of variation (CV) calculations performed using FlowJo V7.2.5 FC analysis software (Tree Star Inc.) to determine the CV, mean size of the gate and means for the standard and sample peaks (Cousin et al 2009). Sample 2C DNA was calculated by (mean of sample peak/mean of standard peak)*2C DNA amount of the standard species (L. sativa: 5.5 pg) (Zonneveld et al 2005). Ploidy x-value was calculated by 2C value/(0.97 +2.36): (B. rapa AA-genome + B. carinata BBCC-genome, respectively).…”

Section: Flow Cytometrymentioning

confidence: 99%

The Fate of Chromosomes and Alleles in an AllohexaploidBrassicaPopulation

et al. 2014

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Production of allohexaploid Brassica (2n = AABBCC) is a promising goal for plant breeders due to the potential for hybrid heterosis and useful allelic contributions from all three of the Brassica genomes present in the cultivated diploid (2n = AA, 2n = BB, 2n = CC) and allotetraploid (2n = AABB, 2n = AACC, and 2n = BBCC) crop species (canola, cabbages, mustards). We used highthroughput SNP molecular marker assays, flow cytometry, and fluorescent in situ hybridization (FISH) to characterize a population of putative allohexaploids derived from self-pollination of a hybrid from the novel cross (B. napus 3 B. carinata) 3 B. juncea to investigate whether fertile, stable allohexaploid Brassica can be produced. Allelic segregation in the A and C genomes generally followed Mendelian expectations for an F 2 population, with minimal nonhomologous chromosome pairing. However, we detected no strong selection for complete 2n = AABBCC chromosome complements, with weak correlations between DNA content and fertility (r 2 = 0.11) and no correlation between missing chromosomes or chromosome segments and fertility. Investigation of next-generation progeny resulting from one highly fertile F 2 plant using FISH revealed general maintenance of high chromosome numbers but severe distortions in karyotype, as evidenced by recombinant chromosomes and putative loss/duplication of A-and C-genome chromosome pairs. Our results show promise for the development of meiotically stable allohexaploid lines, but highlight the necessity of selection for 2n = AABBCC karyotypes.T HE Brassica genus contains the largest number of cultivated crop species of any plant genus (Dixon 2007). Six major crop species are B. rapa (Chinese cabbage, turnip), B. oleracea (cabbage, cauliflower, broccoli), B. nigra (black mustard), B. napus (canola, rapeseed), B. juncea (Indian mustard, leaf mustard), and B. carinata (Ethiopian mustard). These six species share a unique genomic relationship: progenitor diploid species B. rapa (2n = AA), B. oleracea (2n = CC), and B. nigra (2n = BB) gave rise to the allotetraploid species B. juncea (2n = AABB), B. napus (2n = AACC), and B. carinata (2n = BBCC) through pairwise crosses (Morinaga 1934;U 1935). However, despite the fact that each pair of genomes coexists in an allotetraploid species, no naturally occurring allohexaploid species (2n = AABBCC) exists. In general, interspecific hybridization and polyploidy in plants are potent evolutionary mechanisms, allowing formation of new species with adaptation to a wider range of climatic conditions and greater "hybrid vigor" than their progenitor species (Otto and Whitton 2000;Leitch and Leitch 2008). Hence, production of an artificial allohexaploid in the agriculturally important Brassica genus could potentially give rise to new crop types with greater intersubgenomic heterosis ) and tolerance of a wider range of environmental conditions than preexisting Brassica crops (Chen et al. 2011).

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First Nuclear DNA Amounts in more than 300 Angiosperms

Cited by 227 publications

References 20 publications

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

Detailed Recombination Studies Along Chromosome 3B Provide New Insights on Crossover Distribution in Wheat (Triticum aestivum L.)

Are We There Yet? Reliably Estimating the Completeness of Plant Genome Sequences

The Fate of Chromosomes and Alleles in an AllohexaploidBrassicaPopulation

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