First molluscan TNFR homologue in Zhikong scallop: Molecular characterization and expression analysis

Li, Ling; Qiu, Limei; Song, Linsheng; Song, Xin; Zhao, Jianmin; Wang, Lingling; Mu, Changkao; Zhang, Huan

doi:10.1016/j.fsi.2009.07.009

Cited by 41 publications

(10 citation statements)

References 39 publications

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“…In 2009, a TNFa-like sequence was reported for the first time in a mollusc (the abalone Haliotis discus) [217] and, in agreement to earlier reports on the existence of TNF receptors in scallops [218,219], more recent research led to the identification of this multifunctional cytokine also in Ostrea edulis [214]. We now report at least three mussel transcripts pertaining to the TNF ligand superfamily with the canonical presence of an N-terminal transmembrane region.…”

Section: Modulators Of the Immune Responsesupporting

confidence: 85%

An updated molecular basis for mussel immunity

Gerdol

Venier

2015

Fish & Shellfish Immunology

134

163

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Non-self recognition with the consequent tolerance or immune reaction is a crucial process to succeed as living organisms. At the same time the interactions between host species and their microbiome, including potential pathogens and parasites, significantly contribute to animal life diversity. Marine filter-feeding bivalves, mussels in particular, can survive also in heavily anthropized coastal waters despite being constantly surrounded by microorganisms. Based on the first outline of the Mytilus galloprovincialis immunome dated 2011, the continuously growing transcript data and the recent release of a draft mussel genome, we explored the available sequence data and scientific literature to reinforce our knowledge on the main gene-encoded elements of the mussel immune responses, from the pathogen recognition to its clearance. We carefully investigated molecules specialized in the sensing and targeting of potential aggressors, expected to show greater molecular diversification, and outlined, whenever relevant, the interconnected cascades of the intracellular signal transduction. Aiming to explore the diversity of extracellular, membrane-bound and intracellular pattern recognition receptors in mussel, we updated a highly complex immune system, comprising molecules which are described here in detail for the first time (e.g. NOD-like receptors) or which had only been partially characterized in bivalves (e.g. RIG-like receptors). Overall, our comparative sequence analysis supported the identification of over 70 novel full-length immunity-related transcripts in M. galloprovincialis. Nevertheless, the multiplicity of gene functions relevant to immunity, the involvement of part of them in other vital processes, and also the lack of a refined mussel genome make this work still not-exhaustive and support the development of more specific studies.

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Section: Modulators Of the Immune Responsesupporting

confidence: 85%

An updated molecular basis for mussel immunity

Gerdol

Venier

2015

Fish & Shellfish Immunology

134

163

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“…In mollusks, a gene homologous to human TNF-a was described in disk abalone [21] and in the flat oyster (Ostrea edulis) [76], and members of the TNFR family have been identified in Chlamys farreri [67] and Mytilus edulis [97]. Other ligands related to the apoptotic process, such as the TRAIL (Apo2-ligand) and the FasL ligand, have 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 been identified in Crassostrea ariakensis, C. gigas, O. edulis and H. discus discus [22,84,143,147,148].…”

Section: Caspase Activation: the Extrinsic Pathwaymentioning

confidence: 99%

The complexity of apoptotic cell death in mollusks: An update

Romero

Novoa

Figueras

2015

Fish & Shellfish Immunology

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“…Other TNF-related genes have been identified in the Zhikong scallop, such as a TNFR homologue [78] and a tumor necrosis factor receptor-associated factor 6 (TRAF6), which is a key signaling adaptor molecule common to the TNFR superfamily and to the IL-1R/TLR family [79]. Figure 7 shows that several components of the TLR signaling pathway that are present in our transcriptomic sequences (MyD88, IRAK4, TRAF-3 and -6, TRAM, BTK, RAC-1, PI3K, AKT, BTK and TANK).…”

Section: Other Immune Moleculesmentioning

confidence: 99%

Transcriptomics of In Vitro Immune-Stimulated Hemocytes from the Manila Clam Ruditapes philippinarum Using High-Throughput Sequencing

et al. 2012

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BackgroundThe Manila clam (Ruditapes philippinarum) is a worldwide cultured bivalve species with important commercial value. Diseases affecting this species can result in large economic losses. Because knowledge of the molecular mechanisms of the immune response in bivalves, especially clams, is scarce and fragmentary, we sequenced RNA from immune-stimulated R. philippinarum hemocytes by 454-pyrosequencing to identify genes involved in their immune defense against infectious diseases.Methodology and Principal FindingsHigh-throughput deep sequencing of R. philippinarum using 454 pyrosequencing technology yielded 974,976 high-quality reads with an average read length of 250 bp. The reads were assembled into 51,265 contigs and the 44.7% of the translated nucleotide sequences into protein were annotated successfully. The 35 most frequently found contigs included a large number of immune-related genes, and a more detailed analysis showed the presence of putative members of several immune pathways and processes like the apoptosis, the toll like signaling pathway and the complement cascade. We have found sequences from molecules never described in bivalves before, especially in the complement pathway where almost all the components are present.ConclusionsThis study represents the first transcriptome analysis using 454-pyrosequencing conducted on R. philippinarum focused on its immune system. Our results will provide a rich source of data to discover and identify new genes, which will serve as a basis for microarray construction and the study of gene expression as well as for the identification of genetic markers. The discovery of new immune sequences was very productive and resulted in a large variety of contigs that may play a role in the defense mechanisms of Ruditapes philippinarum.

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First molluscan TNFR homologue in Zhikong scallop: Molecular characterization and expression analysis

Cited by 41 publications

References 39 publications

An updated molecular basis for mussel immunity

An updated molecular basis for mussel immunity

The complexity of apoptotic cell death in mollusks: An update

Transcriptomics of In Vitro Immune-Stimulated Hemocytes from the Manila Clam Ruditapes philippinarum Using High-Throughput Sequencing

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