First molluscan TNF-α homologue of the TNF superfamily in disk abalone: Molecular characterization and expression analysis

Zoysa, Mahanama De; Jung, Sung‐Ju; Lee, Jehee

doi:10.1016/j.fsi.2008.10.004

Cited by 84 publications

(53 citation statements)

References 39 publications

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“…TNF-a in vertebrates is a major death factor involved in the extrinsic pathway of caspase activation and induces apoptosis [15,19,28]. In our previous study, we demonstrated TNF-a is mostly expressed in the gill of disk abalone [29]. Our results were similar to those reported for variously-colored abalone caspase and disk abalone TNF-a, suggesting that caspase pathway molecules are highly active in gills than other tissues of un-induced abalones.…”

Section: Discussionsupporting

confidence: 86%

Molluscan death effector domain (DED)-containing caspase-8 gene from disk abalone (Haliotis discus discus): Molecular characterization and expression analysis

Lee

Zoysa

Whang

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 86%

Molluscan death effector domain (DED)-containing caspase-8 gene from disk abalone (Haliotis discus discus): Molecular characterization and expression analysis

Lee

Zoysa

Whang

et al. 2011

Fish & Shellfish Immunology

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“…In 2009, a TNFa-like sequence was reported for the first time in a mollusc (the abalone Haliotis discus) [217] and, in agreement to earlier reports on the existence of TNF receptors in scallops [218,219], more recent research led to the identification of this multifunctional cytokine also in Ostrea edulis [214]. We now report at least three mussel transcripts pertaining to the TNF ligand superfamily with the canonical presence of an N-terminal transmembrane region.…”

Section: Modulators Of the Immune Responsesupporting

confidence: 84%

An updated molecular basis for mussel immunity

Gerdol

Venier

2015

Fish & Shellfish Immunology

134

163

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Non-self recognition with the consequent tolerance or immune reaction is a crucial process to succeed as living organisms. At the same time the interactions between host species and their microbiome, including potential pathogens and parasites, significantly contribute to animal life diversity. Marine filter-feeding bivalves, mussels in particular, can survive also in heavily anthropized coastal waters despite being constantly surrounded by microorganisms. Based on the first outline of the Mytilus galloprovincialis immunome dated 2011, the continuously growing transcript data and the recent release of a draft mussel genome, we explored the available sequence data and scientific literature to reinforce our knowledge on the main gene-encoded elements of the mussel immune responses, from the pathogen recognition to its clearance. We carefully investigated molecules specialized in the sensing and targeting of potential aggressors, expected to show greater molecular diversification, and outlined, whenever relevant, the interconnected cascades of the intracellular signal transduction. Aiming to explore the diversity of extracellular, membrane-bound and intracellular pattern recognition receptors in mussel, we updated a highly complex immune system, comprising molecules which are described here in detail for the first time (e.g. NOD-like receptors) or which had only been partially characterized in bivalves (e.g. RIG-like receptors). Overall, our comparative sequence analysis supported the identification of over 70 novel full-length immunity-related transcripts in M. galloprovincialis. Nevertheless, the multiplicity of gene functions relevant to immunity, the involvement of part of them in other vital processes, and also the lack of a refined mussel genome make this work still not-exhaustive and support the development of more specific studies.

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“…Recent studies in mammals suggest that TNF-␣ has strong antiviral effects. TNF-␣ is also involved in antiviral defense in invertebrates (6). In the absence of specific Abs for Xenopus macrophages, it is not possible to determine whether these cells are the main producers of TNF-␣.…”

Section: Discussionmentioning

confidence: 99%

Innate Immune Responses and Permissiveness to Ranavirus Infection of Peritoneal Leukocytes in the FrogXenopus laevis

Morales

Abramowitz

Gertz

et al. 2010

J Virol

104

135

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Ranaviruses such as frog virus 3 ([FV3]family Iridoviridae) are increasingly prevalent pathogens that infect reptiles, amphibians, and fish worldwide. Whereas studies in the frog Xenopus laevis have revealed the critical involvement of CD8 T-cell and antibody responses in host resistance to FV3, little is known about the role played by innate immunity to infection with this virus. We have investigated the occurrence, composition, activation status, and permissiveness to infection of peritoneal leukocytes (PLs) in Xenopus adults during FV3 infection by microscopy, flow cytometry, and reverse transcription-PCR. The total number of PLs and the relative fraction of activated mononucleated macrophage-like cells significantly increase as early as 1 day postinfection (dpi), followed by NK cells at 3 dpi, before the peak of the T-cell response at 6 dpi. FV3 infection also induces a rapid upregulation of proinflammatory genes including arginase 1, interleukin-1␤, and tumor necrosis factor alpha. Although PLs are susceptible to FV3 infection, as evidenced by apoptotic cells, active FV3 transcription, and the detection of viral particles by electron microscopy, the infection is weaker (fewer infectious particles), more transitory, and involves a smaller fraction (less than 1%) of PLs than the kidney, the main site of infection. However, viral DNA remains detectable in PLs for at least 3 weeks postinfection, past the point of viral clearance observed in the kidneys. This suggests that although PLs are actively involved in anti-FV3 immune responses, some of these cells can be permissive and harbor quiescent, asymptomatic FV3.

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First molluscan TNF-α homologue of the TNF superfamily in disk abalone: Molecular characterization and expression analysis

Cited by 84 publications

References 39 publications

Molluscan death effector domain (DED)-containing caspase-8 gene from disk abalone (Haliotis discus discus): Molecular characterization and expression analysis

Molluscan death effector domain (DED)-containing caspase-8 gene from disk abalone (Haliotis discus discus): Molecular characterization and expression analysis

An updated molecular basis for mussel immunity

Innate Immune Responses and Permissiveness to Ranavirus Infection of Peritoneal Leukocytes in the FrogXenopus laevis

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