First Molecular Identification of Ancylostoma Species in Dogs in a Rural Tea Estate Community in Sri Lanka and the Detection of Other Zoonotic Gastro-intestinal Parasites

Silva, Tilini K De; Rajakaruna, R. S.; Mohotti, K. M.; Rajapakse, Jayanthe; Perera, Piyumali K.

doi:10.1007/s11686-022-00531-7

Cited by 6 publications

(4 citation statements)

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“…Although A. tubaeforme was reported to be endemic to the South Asian region (e.g., India, Thailand, Malaysia and Laos) [35][36][37], this is the rst report of detecting A. tubaeforme in free-roaming dogs in Sri Lanka,. However, among the two Ancylostoma species reported here, A. caninum was the predominant dog hookworm species, consistent with De Silva et al (2022) [16] ndings. Despite having extracted the genomic material from a concentrated pellet of nematode eggs, the nal yields from the PCR amplicons were potentially too low to yield a sequence for further phylogenetic analysis.…”

Section: Discussionsupporting

confidence: 90%

“…Mohotti, personal communication, February 12, 2021). Similarly, De Silva et al (2022) [16] reported comparatively lower FECs in dog faeces in Talawakelle. Existing literature, for example, Okulewicz (2017) and Blum and Hotez (2018) [26,27], also support the notion of the positive correlation between soil temperature and STN infection prevalence.…”

Section: Discussionmentioning

confidence: 85%

“…The sample size was calculated by using a signi cance value of 5% (α) and the expected prevalence of 97% gastrointestinal parasites in dogs De Silva et al (2022) [16] in Sri Lanka. After considering a precision of 5%, the required sample size calculated for a single location was 22, thus 44 for both.…”

Section: Sample Size Calculationmentioning

confidence: 99%

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Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka

Amarasingha,

Anupama,

Rajakaruna

et al. 2024

Acta Parasit.

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Soil-transmitted nematode (STN)s are widespread in tropical and subtropical regions, particularly where the communities are socio-economically challenged. We investigated the effect of soil temperature on the prevalence and intensity of STN infection in free-roaming dogs. MethodsFresh faecal samples collected from free-roaming dogs in Digana and Pussellawa town areas in the Kandy District, Sri Lanka, were microscopically analysed for canine STNs. Soil temperature was measured at each sampling site. Highly prevalent canine hookworm Ancylostoma, was further studied using PCR and sequencing, followed by phylogenetic analysis. ResultsThe soil temperature ranged between 28-31 °C (mean = 29.79 °C) and 18-21 °C (mean = 19.52 °C) in Digana and Pussellawa, respectively, showing a signi cant difference in the two sites (Students t-test t = 1.68, p <0.0001). Of the total 44 dogs sampled, 41 (93.2%) were positive for STNs. During microscopic analysis, ve nematodes: Ancylostoma spp., Capillaria sp., Strongyloides sp., Toxocara canis, and Trichuris sp., were identi ed. Ancylostoma species (93.2%) were the most prevalent, followed by Strongyloides sp. (22.7%) and Toxocara canis (15.9%). Infection prevalence of Strongyloides sp. was higher in Digana (40.9 %) compared to that in Pussellawa (4.5%; Chi-square test, χ 2 = 8.28, p = 0.004) and also the infection intensity from Digana (EPG = 8.02 ± 20.2) compared to that from Pussellawa (0.45 ±2.1; Mann Whitney U test, p = 0.006). Amplicons (ITS1-5.8S-ITS2) of the expected size for A. caninum, and A. tubaeforme were produced. The A. caninum sequence de ned herein (OQ101719) illustrated the highest similarity of 99.2% to one of the local sequences (MZ707153) upon pairwise comparison. ConclusionDigana with higher soil temperature had a signi cantly higher prevalence and infection intensity, particularly Strongyloides sp. This study also signi es the rst molecular identi cation of hookworm species A. tubaeforme in Sri Lanka.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 85%

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Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka

Amarasingha,

Anupama,

Rajakaruna

et al. 2024

Acta Parasit.

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“…Dogs become infected via various routes but commonly through skin penetration of larvae or faecal-oral route from contaminated environment [14]. Trans mammary transmission from an infected mother to puppies has also been reported [15]. Similarly, infection in humans occurs through intradermal penetration of Ancylostoma larvae from a contaminated environment, resulting in cutaneous larva migrans (CLM), also known as creeping eruption, sandworm eruption, plumber's itch, and serpiginous dermatitis [13,16].…”

Section: Introductionmentioning

confidence: 99%

Knowledge and Practices Related to Dog-Associated Zoonoses in Ekiti State, Southwestern Nigeria

Omonijo,

Farayola

2024

PAJOLS

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Background: Human-dog contact has been associated with increased risks of zoonoses. However, dog owners’ knowledge and practice in preventing zoonoses transmission from dogs in Nigeria are limited. This study ex-plored dog owners’ knowledge and practices concerning zoonotic disease transmission. Methods: Semi-structured questionnaires were administered to households owning dogs to assess knowledge and practice of dog-contact-associated zoonoses (DCAZ). This cross-sectional study involved mothers, fathers, and young adults who closely interacted with dogs in households in Ado, Ido, Ikole, and Oye Local Government Areas (LGAs) of Ekiti State, Nigeria, from December 2021 to May 2022. The demographic characteristics of respondents were collected for statistical analysis using the statistical package for social sciences IBM-SPSS version 25.0. The relationship between respondents’ knowledge and demographic features was analysed using the Chi-square test of independence on SPSS. Results were considered significant at p < 0.05. Results: Overall, 200 dog owners participated in the study. The mean age of participants was 32.47 (±10.99). A significant difference (p<0.05) was observed in the number of dogs owned per household. The knowledge of dog contact-associated zoonoses (DCAZ) was estimated at 25.5% (51/200). Association between knowledge of DCAZ and sociodemographic factors showed that age, respondents’ identity, and level of education were signifi-cantly associated (p<0.05) with knowledge being highest among age group 41-50 and respondents with high lev-el of education. The practices of kissing dogs, walking barefooted, eating dog meat, and respondents’ preference for consumption of dog meat were observed to vary significantly among the study locations, with respondents from Ikole ranking higher at 54.0%, 50.0%, 14.0%, and 38.0% respectively (p<0.05). A significant difference was observed in the type of meat the respondents gave to their dogs (p<0.05). Overall, this study showed that 23.5% (47/200) of the respondents consumed dog meat, of which 68.1% (32/47) of the consumers showed a pref-erence for undercooked dog meat (p<0.05). Conclusion: This study showed the low level of knowledge regarding dog contact-associated zoonoses in Ekiti State and the need to intensify awareness of transmission routes and practices that facilitate disease transmission, utilising a One Health approach.

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First record of feline urinary bladder worm (Capillaria syn. Pearsonema) in Sri Lanka: Clinical, histopathological and molecular characterization

De Silva,

Wijeratne,

Perera

et al. 2024

Veterinary Parasitology: Regional Studies and Reports

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First Molecular Identification of Ancylostoma Species in Dogs in a Rural Tea Estate Community in Sri Lanka and the Detection of Other Zoonotic Gastro-intestinal Parasites

Cited by 6 publications

References 53 publications

Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka

Effect of Soil Temperature on Canine Soil-Transmitted Nematodes in Kandy District with the First Record of Hookworm, Ancylostoma tubaeforme from Sri Lanka

Knowledge and Practices Related to Dog-Associated Zoonoses in Ekiti State, Southwestern Nigeria

First record of feline urinary bladder worm (Capillaria syn. Pearsonema) in Sri Lanka: Clinical, histopathological and molecular characterization

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