First molecular identification and genetic characterization of Anaplasma ovis in sheep from Tunisia

Belkahia, Hanène; Saïd, Mourad Ben; Hamdi, Sihem El; Yahiaoui, Mouna; Gharbi, Mohamed; Dâaloul-Jedidi, Monia; Mhadhbi, Moez; Jedidi, Mohamed; Darghouth, Mohamed Aziz; Klabi, Imen; Zribi, Lilia; Messadi, Lilia

doi:10.1016/j.smallrumres.2014.07.009

Cited by 43 publications

(23 citation statements)

References 30 publications

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“…This high discrepancy between prevalence rates may result from differences in tick control programs, farm management, husbandry practices, wildlife reservoir hosts, and/or abiotic factors. In fact, several studies have reported the variability of Anaplasma species prevalence in ruminants according to geographic location, associated with suitable tick habitats and animal management [10,28,43]. Moreover, the infection rate of Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

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Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Belkahia

Saïd

Sayahi³

et al. 2015

J Infect Dev Ctries

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Introduction: Little information is currently available regarding the presence of Anaplasma species in North African dromedaries. To fill this gap in knowledge, the prevalence, risk factors, and genetic diversity of Anaplasma species were investigated in Tunisian dromedary camels. Methodology: A total of 226 camels from three different bioclimatic areas were sampled and tested for the presence of Anaplasma species by quantitative polymerase chain reaction (qPCR) and nested polymerase chain reaction (nPCR) assays. Detected Anaplasma strains were characterized by 16S rRNA sequence analysis. Results: Overall infection rate of Anaplasma spp. was 17.7%, and was significantly higher in females. Notably, A. marginale, A. centrale, A. bovis, and A. phagocytophilum were not detected. Animals were severely infested by three tick species belonging to the genus Hyalomma (H. dromedarii, H. impeltatum, and H. excavatum). Alignment, similarity comparison, and phylogenetic analysis of the 16S rRNA sequence variants obtained in this study suggest that Tunisian dromedaries are infected by more than one novel Anaplasma strain genetically related to A. platys. Conclusions: This study reports the presence of novel Anaplasma sp. strains genetically related to A. platys in dromedaries from various bioclimatic areas of Tunisia. Findings raise new concerns about the specificity of the direct and indirect diagnostic tests routinely used to detect different Anaplasma species in ruminants and provide useful molecular information to elucidate the evolutionary history of bacterial species related to A. platys.

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Section: Discussionmentioning

confidence: 99%

“…Molecular findings demonstrated the occurrence of A. phagocytophilum infections in dogs and horses [26,27], as well as A. ovis in sheep from the northern and central areas of the country [28]. The presence of A. phagocytophilum in horses and dromedaries was investigated by serology [29,30].…”

Section: Introductionmentioning

confidence: 99%

Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Belkahia

Saïd

Sayahi³

et al. 2015

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

show abstract

“…This discrepancy may result from differences in tick control programs, farm management, husbandry practices, wildlife reservoir hosts, and/or abiotic factors. In fact, several studies reported the variability of Anaplasma species prevalence according to geographic location, associated with suitable tick habitats and animal management (Liu et al, 2012;Belkahia et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In small ruminants, the diseases due to the tick-borne pathogens belonging to the genera Anaplasma, Babesia, Theileria and Toxoplasma are widespread in Tunisia (Gharbi et al, 2013;M'ghirbi et al, 2013;Belkahia et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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First molecular survey of Anaplasma bovis in small ruminants from Tunisia

Saïd

Belkahia

Karaoud

et al. 2015

Veterinary Microbiology

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Infection by haemopathogens and tick infestation of sheep during summer season in Constantine region, Northeast Algeria

Foughali

Jedidi

Dhibi

et al. 2021

Veterinary Medicine & Sci

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A study on tick infestation of 43 sheep with clinical symptoms of piroplasmosis and anaplasmosis was carried out during summer seasons of 2016 and 2017 in 34 sheep flocks from Beni Hamidene locality, district of Constantine, Northeast Algeria. Only animals with clinical symptoms of piroplasmosis and/or anaplasmosis were checked for tick infestation. Among the 43 examined sheep, 58 ± 15% were infested by ticks. A total of 185 adult ticks (100 males and 85 females) were collected from the 25 sheep. Two tick genera, Rhipicephalus and Hyalomma consisting of four species were collected, Rhipicephalus bursa was the most frequent tick (164/185; 88.6 ± 4.6%), followed by Rhipicephalus sanguineus sensu lato (16/185; 8.6 ± 4%), Rhipicephalus (Boophilus) annulatus (4/185; 2.2 ± 2.1%) and Hyalomma scupense (1/185; 0.5 ± 1%). Mean overall tick infestation intensity was 7.4. Mixed infestations with two tick species were found in 10 sheep (23.3 ± 12.6%). All farmers (34/34) used ivermectin, and only 11% of them used acaricide to control ticks. The majority of R. bursa ticks (87/185; 45 ± 7%) were located on the ears 37/185; 20 ± 6%) and the testicles (50/185; 27 ± 6%). Giemsa‐stained blood smears examination showed the presence of three haemopathogens: Anaplasma spp. (19/43; 44 ± 15%); Babesia spp. (6/43; 14 ± 10%) and Theileria spp. (1/43; 2 ± 4%). These results showed the presence of several low infestation burdens by ticks transmitting three haemopathogens.

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First molecular identification and genetic characterization of Anaplasma ovis in sheep from Tunisia

Cited by 43 publications

References 30 publications

Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)

First molecular survey of Anaplasma bovis in small ruminants from Tunisia

Infection by haemopathogens and tick infestation of sheep during summer season in Constantine region, Northeast Algeria

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